全文获取类型
收费全文 | 38112篇 |
免费 | 1806篇 |
国内免费 | 4318篇 |
专业分类
林业 | 2752篇 |
农学 | 4072篇 |
基础科学 | 589篇 |
4625篇 | |
综合类 | 17579篇 |
农作物 | 2801篇 |
水产渔业 | 2779篇 |
畜牧兽医 | 5174篇 |
园艺 | 1623篇 |
植物保护 | 2242篇 |
出版年
2024年 | 126篇 |
2023年 | 441篇 |
2022年 | 901篇 |
2021年 | 1040篇 |
2020年 | 1120篇 |
2019年 | 1325篇 |
2018年 | 938篇 |
2017年 | 1570篇 |
2016年 | 1996篇 |
2015年 | 1708篇 |
2014年 | 1312篇 |
2013年 | 1992篇 |
2012年 | 2941篇 |
2011年 | 2867篇 |
2010年 | 2259篇 |
2009年 | 2076篇 |
2008年 | 2343篇 |
2007年 | 2819篇 |
2006年 | 2550篇 |
2005年 | 1927篇 |
2004年 | 1607篇 |
2003年 | 1124篇 |
2002年 | 900篇 |
2001年 | 802篇 |
2000年 | 746篇 |
1999年 | 628篇 |
1998年 | 501篇 |
1997年 | 450篇 |
1996年 | 429篇 |
1995年 | 428篇 |
1994年 | 356篇 |
1993年 | 382篇 |
1992年 | 353篇 |
1991年 | 306篇 |
1990年 | 275篇 |
1989年 | 195篇 |
1988年 | 160篇 |
1987年 | 100篇 |
1986年 | 102篇 |
1985年 | 47篇 |
1984年 | 22篇 |
1983年 | 13篇 |
1982年 | 4篇 |
1981年 | 9篇 |
1980年 | 7篇 |
1979年 | 11篇 |
1978年 | 4篇 |
1963年 | 5篇 |
1956年 | 3篇 |
1955年 | 5篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
141.
142.
核桃早实性状的RAPD分析 总被引:18,自引:0,他引:18
用BSA (Bulked Segregant Analysis) 法获得了与核桃早实性状相关的RAPD 标记OPB208900。混合分离群体的混合样来自早实品种辽1 的自然授粉实生后代。用220 个随机引物扩增早实和晚实混合样, 只有引物OPB208 ( 5. GTCCACACGG 3. ) 在早实混合样及其个体上扩增出了约900 bp 的特异带OPB208900, 而在晚实混合样及其个体上无此特异带。用OPB208 为引物, 以19 个品种和3 个株系的DNA 为模板进行扩增,特异带OPB208900在17 个早实品种中的12 个个体上出现, 而5 个晚实品种( 系) 上均无此带。 相似文献
143.
北方地区小西葫芦黄花叶病毒的酶联检测和西瓜品种抗病性鉴定 总被引:11,自引:2,他引:11
应用DAS-ELISA对分别于1998年在河南、陕西和1999年在河南、陕西、河北和山西采集的葫芦科病毒病样本84份、186份进行小西葫芦黄花叶病毒的检测,表明该病毒在这些地区广泛发生,ZYMV阳性检出率分别为79.8%和57.5%熏在北京和河南临颖县ZYMV发生率较低熏≤23.1%,在其余地区即河北石家庄,河南郑州、开封、孟津,山西运城,陕西西安其阳性检出率在40.0%~92.9%之间。除冬瓜样本没有ZYMV外,西瓜、甜瓜、南瓜、丝瓜、小西葫芦、苦瓜、黄瓜和瓠子均受到ZYMV的侵染。不同地区、年份和作物之间ZYMV阳性检出率存在差异。对18份西瓜品种的抗ZYMV温室人工接种鉴定的试验表明,目前推广的一些品种对ZYMV缺乏抗病性。 相似文献
144.
红肉脐橙(Citrus sinensis L.)果肉中特征色素提取方法探索 总被引:4,自引:0,他引:4
红肉脐橙是目前为止惟一果肉呈粉红色或红色的脐橙,其呈色色素尚未见报道。为深入研究其果肉中特征色素的构成及在果实生长发育和贮存过程中色素含量的变化,本研究在对该色素初步定性的基础上对提取方法进行了探索。在分析红肉脐橙果肉中色素萃取液的紫外可见吸收光谱时发现,该色素有类胡萝卜素的特征吸收峰,将萃取液进行薄层层析则进一步证实其内含有番茄红素。在色素提取的前处理方式上,本研究认为冷冻干燥样品的浸提效果较真空干燥的样品稳定(变异系数小),且浸提率相差无几,所以选择冷冻干燥作为合适的样品前处理方式。色素浸提条件的正交实验结果表明,最佳浸提条件为50℃、1.5h内浸提4次,浸提的料液比为1:60。此外,浸提温度、浸提时间、浸提料液比和浸提次数对浸提率都有显著影响,影响最大的因素是浸提温度,最佳温度为50℃,随温度降低浸提率显著降低。其次是侵提时间的长短,最佳浸提时间为1.5h,延长或缩短0.5h都会使浸提率下降。浸提次数对浸提率的影响是最小的。 相似文献
145.
SSR技术及其在果树上的应用 总被引:27,自引:4,他引:27
SSR(Simple sequence repeat)技术以其丰富的多态性、共显性遗传、重复性好和操作简便等优点日益受到重视,已成为植物遗传和育种研究中不可缺少的分子标记。对SSR技术的原理和特点作了简要的介绍,较详细地分析了如何获得SSR引物,特别是综述了果树上SSR引物的研究现状,同时将其与其它几种主要的分子标记进行了比较分析,认为SSR标记检测的位点多态性水平明显高于RFLP,而且重复性优于RAPD;着重介绍了SSR技术在果树种质资源和构建果树遗传图谱及基因定位等研究中的应用现状;指出SSR技术将在果树科研上起到重要的作用。 相似文献
146.
147.
Spatial dependence and the relationship of soil organic carbon and soil moisture in the Luquillo Experimental Forest,Puerto Rico 总被引:11,自引:0,他引:11
Wang Hongqing Hall Charles A.S. Cornell Joseph D. Hall Myrna H.P. 《Landscape Ecology》2002,17(8):671-684
We used geo-spatial statistical techniques to examine the spatial variation and relationship of soil organic carbon (SOC) and soil moisture (SM) in the Luquillo Experimental Forest (LEF), Puerto Rico, in order to test the hypothesis that mountainous terrain introduces spatial autocorrelation and crosscorrelation in ecosystem and soil properties. Soil samples (n = 100) were collected from the LEF in the summer of 1998 and analyzed for SOC, SM, and bulk density (BD). A global positioning system was used to georeference the location of each sampling site. At each site, elevation, slope and aspect were recorded. We calculated the isotropic and anisotropic semivariograms of soil and topographic properties, as well as the cross-variograms between SOC and SM, and between SOC and elevation. Then we used four models (random, linear, spherical and wave/hole) to test the semi-variances of SOC, SM, BD, elevation, slope and aspect for spatial dependence. Our results indicate that all the studied properties except slope angle exhibit spatial dependence within the scale of sampling (200 – 1000 m sampling interval). The spatially structured variance (the variance due to the location of sampling sites) accounted for a large proportion of the sample variance for elevation (99%), BD (90%), SOC (68%), aspect (56%) and SM (44%). The ranges of spatial dependence (the distances within which parameters are spatially dependent) for aspect, SOC, elevation, SM, and BD were 9810 m, 3070 m, 1120 m, 930 m and 430 m, respectively. Cross correlograms indicate that SOC varies closely with elevation and SM depending on the distances between samples. The correlation can shift from positive to negative as the separation distance increases. Larger ranges of spatial dependence of SOC, aspect and elevation indicate that the distribution of SOC in the LEF is determined by a combination of biotic (e.g., litterfall) and abiotic factors (e.g., microclimate and topographic features) related to elevation and aspect. This demonstrates the importance of both elevation and topographic gradients in controlling climate, vegetation distribution and soil properties as well as the associated biogeochemical processes in the LEF.This revised version was published online in May 2005 with corrections to the Cover Date. 相似文献
148.
S.K. Raj R. Singh S.K. Pandey B.P. Singh 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(5):467-470
Natural occurrence of a geminivirus causing severe leaf curl disease on sunn hemp (Crotalaria juncea) was recorded in India. The association of a geminivirus with the disease was demonstrated by whitefly transmission tests and polymerase chain reaction (PCR) amplification of DNA fragments of expected sizes with three pairs of degenerate geminivirus primers. The PCR-amplified viral DNA fragments were further characterized by Southern hybridization with a geminivirus probe consisting of the cloned coat protein (CP) gene of Indian tomato leaf curl virus (ITLCV). Restriction fragment length polymorphism analysis of a PCR-amplified CP fragment revealed that the geminivirus from sunn hemp was different than ITLCV. 相似文献
149.
Approach of allelopathy study with Arabidopsis thaliana (L.) Hevnh. and Neurospora crassa 总被引:2,自引:0,他引:2
KAORI TOMITA-YOKOTANI TAKAKO KATO MOHAMMAD MASUD PARVEZ YASUHEI MORI NOBUHARU GOTO KOJI HASEGAWA 《Weed Biology and Management》2003,3(2):93-97
Allelopathy in Arabidopsis seeds was investigated in the present study because there are few available reports of allelopathy studies regarding the early development stage of the plant life cycle, and because Arabidopsis is widely used as a model plant for molecular biology. When cockscomb ( Celosia cristata ) was incubated together with Arabidopsis seeds, the growth of cockscomb was promoted by Arabidopsis seeds. Exudate of Arabidopsis seeds showed a promotive activity on the growth of cockscomb and Arabidopsis itself. A new method for studying allelopathic mechanisms involving the interaction of the plant Arabidopsis thaliana (L.) Hevnh. and the fungus Neurospora crassa was suggested. Arabidopsis seed exudate and the water-soluble fraction of the exudate promoted Arabidopsis growth and reduced fungal germination, indicating that the seed exudate has a species-selective activity. Conversely, the ethylacetate-soluble fraction inhibited growth of all tested materials in the current study. These results demonstrate that the water-soluble substance(s) released from Arabidopsis seeds have species-selective activity on growth of both plants and microorganisms. 相似文献
150.