3株鸽源新城疫病毒的分子特征及对鸽的致病性

2011-2013年在新城疫流行病学调查中分离到3株鸽源新城疫病毒(SDS,SD01和SD02),为了进一步了解其生物学特性和遗传进化规律,对3株病毒进行了测序和生物活性分析,并对分离株SDS对鸽的致病性进行了评价.结果表明,毒株SDS基因组全长为15 192 bp,基因排列方式为3'-NP-P-M-F-HN-L-5'...     

Role of miR-219 and TGFBR2 in pathogenesis of renal fibrosis

AIM:To study the role of microRNA-219 (miR-219) in regulation of transforming growth factor-β receptor type 2 (TGFBR2) in renal fibrosis. METHODS:The renal fibrosis patients (n=70) were selected in this stu-dy, and 20 cases of healthy people were selected as control group. RT-qPCR was used to detect the expression of miR-219 in the serum of the patients with renal fibrosis and control group, and the expression of miR-219 in NRK49F cells after stimulation with angiotensin Ⅱ(AngⅡ) was detected. The protein expression of α-smooth muscle actin (α-SMA) in the NRK49F cells transfected with miR-219 mimics after stimulation with AngⅡ was determined by Western blot. The potential target gene TGFBR2 of miR-219 was screened and verified by the method of luciferase reporter gene. RT-qPCR and Western blot were used to detected the effect of miR-219 mimics on the expression of TGFBR2 at mRNA and protein levels, and the mRNA expression of α-SMA, connective tissue growth factor (CTGF), type I collagen α1 (COL1A1) and COL3A1 in the NRK49F cells was also detected, respectively. The unilateral ureteral occlusion (UUO) mouse model was established and the expression of miR-219 in the renal tissue was monitored. The morphological change of renal fibrosis was observed in the UUO mice after injection of miR-219, and the mRNA expression levels of COL1A1 and COL3A1 were detected. RESULTS:The expression level of miR-219 in the patients with renal fibrosis was significantly lower than that in control group, and the expression of miR-219 in the UUO mice was decreased significantly (P<0.01). The expression level of miR-219 was significantly decreased in the NRK49F cells after AngⅡ stimulation, and miR-219 mimics inhibited the protein expression of α-SMA(P<0.01). miR-219 mimics had a targeted regulatory effect on TGFBR2 gene, which inhibited the mRNA and protein expression of TGFBR2. miR-219 mimics inhibited the mRNA expression of α-SMA, CTGF, COL1A1 and COL3A1. miR-219 also down-regulated the mRNA expression of COL1A1 and COL3A1 in the UUO mice and inhibited the process of renal fibrosis. CONCLUSION:miR-219 inhibits the development of renal fibrosis by inhibiting the expression of TGFBR2, which may become a new target for the diagnosis and treatment of renal fibrosis.     

莼菜资源利用研究综述及展望

通过分析莼菜的生物学特性、营养特性,对莼菜在营养保健、医学价值、治理污水、净化水质方面的研究现状进行了综述,并对今后的研究方向提出了展望,以期为人们综合利用莼菜资源、为莼菜的深加工应用提供科学依据。     

应用逆转录套式PCR检测新城疫病毒核酸

选择DNV融合蛋白保守的编码区域,设计并合成了一对外引物和一对内引物,建立并优化了检测新城疫病毒核酸的逆转录套式PCRI地,通过检测NDV感染的实验客观存在病料和临床病料,结果表明,逆转录套式PCR法最低能鉴别出约0.3pg的NDV RNA,攻毒后第8天还能从非免疫鸡和SPF鸡泄殖腔拭子中检出DNV,第8天非免疫鸡泄殖腔拭子中NDV的最大检出率为5/10,第8天SPF鸡泄殖腔拭子中NDV的最大检出率为6/10,对非免疫鸡和SPF鸡的泄殖腔中NDV最佳检出时间均在攻毒后第5天。逆转录套式PCRY应运地临床样品中DNV的最大检出率为6/7,经核酸杂交验证,该法具有很高的特异性和敏感性,也比较简便快速,为从分子水平探讨NDV的发病机理、临床早期快速诊断提供了新的研究手段。     

Characterization of polymorphisms of transferrin receptor and their association with susceptibility to ETEC F4ab/ac in pigs

Diarrhoea caused by enterotoxigenic Escherichia coli (ETEC) expressing F4 (F4ab, F4ac and F4ad) fimbriae is a significant cause of mortality and morbidity in newborn and weaned pigs. The locus controlling susceptibility towards ETEC F4ab/ac has been mapped to SSC13q41, in which TFRC (transferrin receptor) was localized and considered as a positional candidate gene for ETEC F4ab/ac receptor. In this study, we determined susceptibility/resistance to ETEC F4ab/ac in a total of 755 F2 animals from a White Duroc x Erhualian intercross using a microscopic enterocyte adhesion assay. We identified two TFRC polymorphisms (SNPs 591 A>G and 632 A>G) in a single exon after comparative sequencing analysis of 2371-bp amplicons containing the complete coding region of TFRC using RNA of eight full-sib F2 animals with susceptible and resistant phenotypes. The intron sequences flanking the two exon polymorphisms were obtained, revealing an intron polymorphism (SNP 291 C>T). We genotyped the 19 founder animals of the White Duroc x Erhualian intercross for the identified polymorphisms, showing that only the 291 C>T polymorphism is a highly informative marker. We further genotyped all 59 F1 and 755 F2 animals for the 291 C>T polymorphism, and the association of this polymorphism with susceptibility/resistance to ETEC F4ab/ac in these F2 animals was evaluated by the transmission disequilibrium test. The result showed that the 291 C>T polymorphism is not a causal mutation, however, has a significant linkage disequilibrium with the ETEC F4ab/ac, especially F4ac receptor locus.     

丰蜜3号厚皮甜瓜的选育

丰蜜3号厚皮甜瓜是以自交系M3为母本、自交系0063为父本配制而成的短椭圆形厚皮甜瓜一代杂种。中早熟,果实发育期30-35d。单果重1.4kg左右,每667m2产量约2200kg。皮色浅黄,覆10条浅绿色纵沟,果面具细网纹;果肉白色,肉厚约3.5cm,肉质结实稍脆,味清香,果实中心可溶性固形物含量17%左右。     

早熟紫长茄新品种玫茄1号的选育

玫茄１号的母本是从台湾紫长茄中选育出的自交系２１－３,父本是从福建地方品种红茄中选育出的自交系２１－４,其杂种一代表现早熟,比对照闽茄１号早收７ｄ（天）,果长３０～３３ｃｍ,稍弯,果径４ｃｍ,单果重１８０ｇ,果皮鲜紫色,果肉乳白色,肉质细嫩,商品性好。一般每公顷产３０～４５ｔ,比对照增产１９．５％。该品种已在闽、浙、赣、粤等省推广,种植面积达３２００ｈｍ２。     

厚皮甜瓜新蜜13号(新皇后)的选育

新蜜１３号一代杂种的父本８９－４和母本８９－１均来自新疆当地厚皮甜瓜红心脆与欧美类型厚皮甜瓜的杂交后代。该品种单瓜重３ｋｇ以上,果实椭圆形,成熟果果皮金黄色,果面网纹细致,果肉浅桔红色,中心折光糖１４度以上,肉质细、脆、香味浓郁,较耐贮运。成熟期比对照品种皇后提前５～１０天,一般６６７ｍ２产量可达２０００～２５００ｋｇ,对甜瓜枯萎病、蔓枯病、疫霉病有较强的抗性。     

强雌性华北型黄瓜新品种青研密刺1号的选育

青研密刺1号是96481-1-自混-1-混和2003A自混配制而成的优良一代杂种。植株长势强,主蔓结瓜为主。瓜长圆筒形,瓜皮深绿色,瓜条顺直,表面光滑无棱沟,白刺瘤、中等大小、多。平均瓜长25.8cm,单瓜质量135g,瓜把长3.9cm,肉厚占横径的比例在60%左右。田间表现抗白粉病和枯萎病。商品性好,风味品质优良。平均667m2产量5020.05kg,比对照津优35增产68.67%,差异极显著。     

我国玉米穗腐病致病镰孢种群及禾谷镰孢复合种的鉴定

为阐明中国玉米镰孢穗腐病的主要致病镰孢菌种类及其分布特征,采用形态学、培养特征及特异性分子鉴定方法,对采集自我国18省100个县的玉米籽粒样品进行分离鉴定,并通过TEF-1α基因序列测定解析禾谷镰孢复合种的构成.结果表明,在我国引起玉米穗腐病的主要致病菌为镰孢菌,分离频率为56.0%,其次还有青霉菌、曲霉菌、木霉菌等.138个镰孢菌分离物中鉴别出7个种及复合种,其中拟轮枝镰孢菌Fusarium verticillioides(56.5%)和禾谷镰孢复合种F.graminearum species complex(37.7%)为广泛分布的优势致病种类,其余为黄色镰孢菌F.culmorum(2.2%)、层出镰孢菌F.proliferatum(1.5%)、尖镰孢复合种F.oxysporum species complex(0.7%)、茄镰孢复合种F.solani species complex(0.7%)和亚粘团镰孢菌F.subglutinans(0.7%).在禾谷镰孢复合种中鉴定出3个独立种:广泛分布的禾谷镰孢菌F.graminearum sensu stricto(59.6%)、分布在云南、贵州及陕西商洛等南方生态区的南方镰孢菌F.meridionale(25.0%)和分布在内蒙古、吉林、山西、河北及北京等北方生态区的布氏镰孢菌F.boothii(11.5%).