Intensification or extensification? Factors affecting technology use in peri-urban lowlands along an agro-ecological gradient in West Africa

The paper analyses whether the increased use of lowlands in proximity to urban centres in West Africa is associated with technological intensification or extensification. Technologies are typified in terms of their orientation – land or labour saving – and market dependence for their procurement and reviews the factors driving and modifying their use in lowlands. The factors associated with technology use are analyzed empirically using geo-referenced lowland data around four urban centers along an ecological gradient in Côte d’Ivoire and Mali. The technologies analyzed include both external inputs (fertilizer, pesticide, herbicide, improved varieties) and crop management practices (plot level bunding, rice transplanting and rice direct seeding). Results from binomial logit models highlight the prominent positive and non-linear role of proximity to urban markets for the technological intensification of lowlands – an issue associated with factor scarcities and the transaction costs for procurement and marketing. Technology use in lowlands is also associated with the agro-ecological gradient, lowland development and non-native lowland users. A common thread linking these variables is that they modify resource scarcity and therefore intensification incentives. Lowlands are not always as valuable as they may seem and there may be limited incentives to intensify. Instances of significant lowland cultivation and intensification in the study sites tend to be associated with specific opportunities – driven by market opportunities and modified by seasonal, institutional or development-induced relative land scarcity. Market access is therefore a necessary but not sufficient factor for the technological intensification of lowland use. There is a need for better targeting of development efforts in terms of enabling lowland intensification or extensification as appropriate.     

成品油管道水击超前保护优化方案

成品油管道发生泵站意外停电事故后通常执行水击超前保护程序,该保护方案对管道的安全性有决定性影响。为了确保管道从事故前稳态安全平稳过渡到事故后较优的运行状态,需要制定最佳的水击超前保护方案。对成品油管道泵站意外停电水击工况进行分析,以水击事故发生后下一稳态时输量最大且各调节阀节流压力之和最小为目标,建立优化模型,相应的约束条件为全线不超压、不汽化。模型求解采取两种不同策略:事故站下游以各站出站压力最低为目标分别寻优,采用水力坡降线平移法求解;事故站上游以整体总节流最小寻优,为了提高求解速度,采用深度优先搜索法求解。以某成品油管道泵站意外停电事故工况为例,对该优化模型进行实例应用,并根据优化结果制定水击事故过渡过程的控制逻辑,并使用仿真软件SPS验证了控制逻辑的合理性,表明该优化模型合理可靠。     

草鱼TAB1蛋白的原核重组表达及其抗体制备

为了制备草鱼重组TAB1蛋白(rCiTAB1)及其特异性抗体,首先以草鱼头肾组织c DNA为模板,PCR扩增Ci TAB1基因全长序列,并依次构建重组克隆质粒p MD19-T-Ci TAB1与重组表达质粒pET-32a-Ci TAB1。该重组表达质粒经0.5 mmol·L~(-1) IPTG,37℃诱导表达12 h后获得以包涵体形式表达的重组CiTAB1蛋白(rCiTAB1)。然后,采用3种不同方法对包涵体蛋白进行变性,发现与高浓度尿素直接变性法和洗涤后尿素变性法相比,洗涤后梯度尿素变性法处理后的蛋白纯度最好,经透析复性后得到浓度为2 mg·mL~(-1)的r Ci TAB1。最后,将rCiTAB1蛋白与白油佐剂及免疫增强剂混合,室温下混合1.5h乳化成免疫原,3次免疫新西兰大白兔制备兔抗CiTAB1抗体,经间接ELISA方法和免疫琼脂双向扩散试验测得免疫后第33天血清中特异性抗体的效价分别为1∶1 048 576和1∶16。Western blot检测到一条分子量约为72 kDa的特异性条带,表明该抗体能特异性识别r Ci TAB1蛋白。研究结果为后续深入研究CiTAB1蛋白功能提供了物质基础。     

虾塘水体中浮游植物群落特征及其与营养状况的关系

通过对虾养殖期间的连续监测结果;讨论和阐述了虾塘水体中浮游植物群落特征及其与营养状况的关系。虾塘中浮游植物在生态学上主要表现为群落构成简单、优势种单一且演替速度快,从而决定了该环境中浮游植物群落的不稳定性。无机氮营养盐（IN）是浮游植物繁殖生长的限制性因素,二者之间里较好的幂函数衰减曲线关系（R＝0．76）,且当浮游植物密度降至3．2×103细胞／ml时,水环境中的IN呈跃升趋势,此时所对应的透明度为0．6±0．2m。     

水温对怀头鲇血液学指标的影响

本实验设定4℃、9℃、14℃、18℃、24℃和30℃6个水温梯度,测定了水温变化对二龄怀头鲇血液生理生化指标的影响。结果显示,水温4～30℃范围内,怀头鲇血红蛋白含量随着水温的升高呈波浪型变化,但变化幅度较小;而血沉与血红蛋白含量变化趋势相反,变化幅度较大;血液中的红细胞数量随水温的升高先增加再减少;血清中的总蛋白、白蛋白和球蛋白的变化趋势一致,都是随水温的升高其含量先增加再减少,而且在9～18℃区间变化较急剧;血清中的血糖,高密度脂蛋白,谷草转氨酶,三碘甲状腺氨酸,甲状腺素,促甲状腺激素,钾和镁八项指标在各水温之间不存在显著差异（P〉0．05）;血清中的尿素氮,甘油三脂,总胆固醇,低密度脂蛋白,谷丙转氨酶,碱性磷酸酶,钠,钙,氯化物和渗透压在部分水温之间存在显著差异（P〈0．05）。     

南昌市3个城中湖泊夏季轮虫和甲壳类群落特征及水质评价

为探究城市湖泊浮游动物群落结构特征和分布与水环境因子之间的关系,并评价南昌市典型城市湖泊夏季水质状况,运用多维尺度分析（MDS）和典范对应分析（CCA）方法,对南昌3个城市湖泊———东湖、青山湖、月亮湖的轮虫和甲壳类浮游动物群落进行了研究,分析了浮游动物的多样性指数。结果表明,轮虫是夏季城市湖泊中浮游动物的优势类群,优势度（Y）大于0．02的浮游动物分别为剪形臂尾轮虫（Brachionus forficula）、裂足臂尾轮虫（Brachionus diversicornis）、萼花臂尾轮虫（Brachionus calyciflorus）、桡足类无节幼体（Copepoda nauplii）和卜氏晶囊轮虫（Asplanchna brightwelli）;3个湖泊之间浮游动物的群落结构差异显著（P ＜0．05）,明显划分为东湖群落、青山湖群落和月亮湖群落。叶绿素 a 浓度、浊度和溶氧是浮游动物分布的显著影响因子（P ＜0．05）。多样性指数评价显示,东湖为清洁型水体,青山湖为清洁－中污型水体,月亮湖为轻污－中污型水体。综合评价表明,东湖和青山湖水体为寡营养水平,月亮湖为中富营养水平。     

渤海鱼类群落结构及其主要增殖放流鱼类的资源量变化

基于2010年5月和8月渤海渔业调查数据,结合1959年以来渤海鱼类相关文献资料,对渤海鱼类群落结构的演替、主要增殖放流鱼类的资源动态作了初步分析.结果表明,渤海鱼类群落优势种由个体大、营养层次高的带鱼Trichiurus haumela、半滑舌鳎Cynoglossus semilaevis等优质底层鱼类逐渐被黄鲫Setipinna taty、鳀Engra ulis ja ponicus等个体小、营养层次低的小型中上层鱼类所替代,并且除黄鲫外,其他小型鱼类优势种也在不断更替中.另外,生物健康度指数(BHI)分析显示,渤海鱼类群落处于相对不稳定状态.鱼类资源量急剧降低,尤其在5月,CPUE由1959年的186 kg/h下降至目前不足1 kg/h,鱼类资源密集区主要分布在莱州湾、渤海湾和渤海中部,而辽东湾分布相对较少,并且鱼类资源与海蜇呈交错分布.近年来,渤海增殖放流的鱼类仅有花鲈Lateolabrax japonicus和许氏平鲉Sebastes schlegeli的资源量有小幅度回升,其他种类从20世纪80年代开始资源量一直处于下降趋势.从渤海鱼类群落结构的演替和增殖放流鱼类的资源量变化来看,渤海鱼类群落依旧是小型经济价值较低的种类控制其能流结构.除花鲈外,其他增殖放流鱼类的资源量均不足历史最高资源量的1％,其资源量的恢复尚有很大的空间,增殖潜力很大.     

虾蟹混养池塘浮游植物群落结构的变化特征

为摸清锯缘青蟹(Scylla serrata)与斑节对虾(Penaeus monodon)混养池塘浮游植物种群结构的变化特征,于2018年3-11月对广州市番禺区十六涌3个养殖池塘中的浮游植物开展了逐月调查,并通过冗余分析(RDA)探讨了浮游植物与环境因子的相关性。结果表明,养殖池塘中共鉴定出浮游植物29种,主要由硅藻、蓝藻、甲藻、绿藻和裸藻5大门类组成,其中硅藻种类最多(19种),占浮游植物种类数的65.5%;其次是蓝藻,共5种,占浮游植物种类数17.2%;甲藻3种,绿藻和裸藻各1种。共出现优势种16种,主要为直舟形藻(Navicula directa)、奇异菱形藻(Nitzschia paradoxa)、柔弱菱形藻(Nitzschia delicatissima)、尖布纹藻(Gyrosigma acuminatum)、大角角藻(Ceratium macroceros)和小颤藻(Oscillatoria tenuis)等。浮游植物细胞密度介于2.56×103~189.2×103个·m-3,Shannon-Weaver多样性指数介于0.468~2.597。浮游植物与环境因子相关性RDA结果表明,营养盐、盐度和pH是影响养殖塘浮游植物种群结构的主要环境因子。     

浙江六横岛泥滩潮间带大型底栖动物的群落特征

本文分析了浙江六横岛泥滩潮间带大型底栖动物的种类组成、数量分布和多样性指数等群落特征。3个泥滩潮间带断面调查采获的大型底栖动物共有19种,其中软体动物10种,节肢动物6种,脊索动物2种,环节动物1种。3个泥滩潮间带断面总平均生物量为470.77±314.32 g/m2, 总平均栖息密度为480±248 ind./m2。在各类群底栖动物中,平均生物量及密度都以软体动物居首位。六横岛潮间带大型底栖动物的Shannon-Wiener多样性指数、Margalef丰富度指数和Pielou均匀度指数的分布范围分别在1.97-2.14、1.45-1.67和0.83-0.95,平均值分别为2.06±0.08、1.53±0.12和0.90±0.06。六横潮间带大型底栖动物ABC曲线分析表明底栖动物群落已受到一定程度的污染扰动。     

Effect of Bacillus cereus as a water or feed additive on the gut microbiota and immunological parameters of Nile tilapia

We evaluated the effects of Bacillus cereus, as an additive in water and feed, on the gut microbiota and immunological parameters of Nile tilapia (Oreochromis niloticus) fingerlings. Experiments were performed in tanks and net cages respectively. Experiment 1: Tilapia were housed in tanks for 42 days, and B. cereus was added to the water at 1.0 × 10⁴ cfu mL^?1 (Treatment 1) and 1.0 ×10⁵ cfu mL^?1 (Treatment 2) weekly. For the control, no probiotic was added. Experiment 2: Tilapia were housed in cages for 42 days, and the feed was supplemented with B. cereus at 1.0 × 10⁷ cfu g^?1 (Treatment 1) and 1.0 × 10⁸ cfu g^?1 (Treatment 2) weekly. For the control, no probiotic was added. Each treatment contained three replicates, with 50 male tilapias per replicate. The fish from the probiotic treatments in both tank and cage experiments had significantly higher serum lysozyme and peroxidase activities than the control. In the cage experiment, alkaline phosphatase and total superoxide dismutase activities in tilapia were significantly higher in probiotic treatments compared with the control. The results of polymerase chain reaction‐denaturing gradient gel electrophoresis showed that B. cereus supplementation in the feed and water affected the autochthonous gut bacteria community of tilapia and stimulated various potentially beneficial bacteria. Therefore, B. cereus, as a water or feed additive, could enhance the immune status and affect the gut microbiota of tilapia. Bacillus cereus was more effective as a feed supplement rather than a water additive for enhancing the immune status of tilapia.