扬州园林植竹造景史考

根据历史文献资料,探讨了扬州园林的植竹造景历史。扬州历代园林竹景建造的特点主要是以竹名园、以竹名景和以竹辅景。     

浅谈日本福冈爱蓝岛城中央公园国际交流庭园

广州园是广州市政府应福冈市政府的邀请,在爱蓝岛城中央公园国际交流庭园展区中展览的岭南园林庭园。占地350m2。运用岭南园林的造园手法,以岭南民居、山池树石、诗文点染相结合,营造出“小中见大”、景观丰富的庭园,充分展现了岭南园林的艺术魅力。     

Porcine circovirus type 2 decreases the infection and replication of attenuated classical swine fever virus in porcine alveolar macrophages

Recently, it has been noted that porcine circovirus type 2 (PCV2) infection adversely affects the protective efficacy of Lapinized Philippines Coronel (LPC) vaccine, an attenuated strain of classical swine fever virus (CSFV), in pigs. In order to investigate the possible mechanisms of the PCV2-derived interference, an in vitro model was established to study the interaction of LPC virus (LPCV) and PCV2 in porcine alveolar macrophages (AMs). The results showed that PCV2 reduced the LPCV infection in AMs and the levels of PCV2-derived interference were dose-dependent. The PCV2-derived interference also reduced the replication level of LPCV in AMs. The full-length PCV2 DNA and its fragment DNA C9 CpG-ODN were involved in the reduction of LPCV infection in AMs, whereas UV-inactivated PCV2 was not. In addition, a moderate negative correlation between the LPCV antigen-containing rate and IFN-γ production was observed, and had a dose-dependent trend with the level of PCV2-inoculation. The results of the present study may partially explain how PCV2 infection interferes with the efficacy of LPC vaccine.     

Comparison of two real-time RT-PCR assays for differentiation of C-strain vaccinated from classical swine fever infected pigs and wild boars

Classical swine fever is one of the most important infectious diseases for the pig industry worldwide due to its economic impact. Vaccination is an effective means to control disease, however within the EU its regular use is banned owing to the inability to differentiate infected and vaccinated animals, the so called DIVA principle. This inability complicates monitoring of disease and stops international trade thereby limiting use of the vaccine in many regions. The C-strain vaccine is safe to use and gives good protection. It is licensed for emergency vaccination in the EU in event of an outbreak. Two genetic assays that can distinguish between wild type virus and C-strain vaccines have recently been developed. Here the results from a comparison of these two real-time RT-PCR assays in an interlaboratory exercise are presented. Both assays showed similar performance.     

Up-regulated expression of PD-1 and its ligands during acute Classical Swine Fever virus infection in swine

In order to investigate the relationship between the PD-1 pathway and impairment of immune responses with the CSFV infection, the mRNA expression of PD-1 and its ligands were evaluated by quantitative polymerase chain reaction (qPCR) during artificial CSFV infection. Simultaneously, expression of IL-2 and IL-10 mRNA were detected. The T cell proliferation and CSFV load in plasma were also measured. Results showed that the expression of PD-1 and its ligands mRNA were significantly increased (p < 0.01) in PBMC from 3 to 7 days post infection (dpi). Meanwhile the level of IL-10 was up-regulated (p < 0.01). The IL-2 mRNA was not obviously changed but it is significantly increased from 14 dpi. The T cell proliferation was notably decreased at 7 dpi. The CSFV load was also increased in plasma. Overall, our results suggest that the expression of PD-1 and its ligands were up-regulated and probably correlated with immune inhibition during acute CSFV infection.     

某猪场猪瘟免疫程序的调整及免疫效果评价

为了更好地预防和控制猪瘟,找出适合四川某猪场的猪瘟免疫程序,笔者根据实际情况对免疫程序进行了调整。然后用ELISA和IHA法对免疫程序调整前后的不同日龄猪血清进行了抗体检测,结果显示:调整前用ELISA法检测到3日龄、25日龄、50日龄、90日龄和120日龄猪只的猪瘟抗体阳性率分别为80%、54.54%、77.78%、80%和95%,用IHA法测得的阳性率分别为100%、63.64%、77.78%、90%和95%;调整后用ELISA法测得的猪瘟抗体阳性率分别为100%、80%、77.27%、77.78%和95%,用正向IHA法测得的抗体阳性率分别为97.73%、94.44%、96.15%、100%和100%。调整前用ELISA和IHA测得的平均阳性率为77.46%、85.28%,调整后则变为86.01%、97.66%,虽然均符合农业部颁布的猪群猪瘟抗体阳性率应不低于70%的标准,但免疫程序调整后的抗体阳性率明显高于调整前,可见调整后的免疫程序更适合于该猪场。     

种猪场猪瘟和伪狂犬病净化策略浅析

猪瘟和伪狂犬病作为两种不同类别的重要动物疫病在我国种猪场仍普遍存在,直接或间接影响着种猪行业的健康发展。近年来,随着猪瘟和伪狂犬病的综合防控措施及诊断技术的不断突破与革新,为猪瘟和伪狂犬病的净化工作奠定了基础,同时两病净化作为一项重要项目前已被列入国家中长期动物疫病防治规划。本文结合监测技术同疫病防控,旨为种猪场猪瘟和伪狂犬病的净化提供相适应的技术路线。     

一起猪圆环病毒病并发猪瘟诊断分析

某市某猪场发生了一起以断奶后保育猪突然发病,发热,皮肤发红,贫血,下痢,衰竭为主要症状,发病率和死亡率很高的急性传染病,经流行病学调查、临床症状、剖检变化、实验室检查等综合诊断,确诊为猪圆环病毒病并发猪瘟混合感染,通过采取合理用药和防制措施,病情得到较好的控制。     

吴地剑舞考

作为吴地文化的重要载体之一,剑舞具有其独特的中国传统文化特色。论述了吴文化背景推动下吴地剑舞的产生过程及其与吴文化的历史关联,阐述了剑舞在平稳发展期、鼎盛时期、多元融合期以及当代发展时期的不同状态,详细论述了剑舞在唐代的表演形式和表演风格,总结了剑舞的文化特色和发展规律。     

园林施工案例解析和管理要点

指出了施工管理是工程项目管理中最重要的组成部分,是对施工准备、施工、竣工审计等整个过程的合理优化。分析了施工过程中的难点、要点及亮点,对组织、质量、进度、成本、安全以及资料等管理方面进行了项目探讨,阐述了管理要点。重点对景观理念的特色、技术创新、团队建设、成本控制等环节进行了研究。