苹果银叶病的发生与防治研究

苹果银叶病的传播途径包括孢子侵染伤口传播、菌丝传播和根系传播.采用发明专利系列治疗药物注孔和灌根并用,按指示剂、治疗剂、排毒剂的顺序用药可以根治苹果银叶病.碱性药物波尔多液和石硫合剂有抵消药效的作用,不能用作保护剂.     

不同剂量伊曲康唑制剂对犬血常规及肝肾功能的影响

旨在评价不同剂量伊曲康唑-环糊精制剂通过皮下注射途径对健康比格犬肝肾功能及血常规的影响.选用体重±10 kg的健康纯种比格犬32只,按试验要求随机分为4组,每组8只,建立稳定的生活制度.分别按照0.25 mg/kg、0.75 mg/kg和1.25 mg/kg剂量,皮下注射伊曲康唑制剂,3日1次,连续7次,对照组注射生理...     

Gene Expression in Black Tiger Prawns Following Intramuscular Injection of β-gal Plasmid

Gene expression in black tiger prawns (Penaeus monodon) was studied following intra-muscular injection of CMVGal plasmid into the second abdominal segment. We used an in situ staining technique to detect -gal expression in one- and three-month-old injected prawns. We found that only one of the three-month-old prawns expressed the marker gene (2 days after injection), and the site of expression was confined to the sixth abdominal segment away from the injection site. We repeated the experiment on a new batch of three-month-old prawns, but using fluorometric determination technique. This time we found that -gal expression was detected (6/42) at the site of injection after 2, 7, and 14 days. In two other test samples, transgene expression was detected in the sixth abdominal segment only, further confirming the possibility of injected DNA dispersal. The results of the study also suggest that direct gene transfer is a feasible technique in black tiger prawns.     

The Effects of Light Salting on Physicochemical Characteristics of Frozen Cod (Gadus morhua) Fillets

Abstract

The effects of light salting by brine injection and brine immersion on physicochemical and textural properties of cod fillets were evaluated. Light salting significantly increased yield and water holding capacity. Adding brine injection to the process can be used to shorten the process time needed to obtain the desired salt content and increase yield. The effects on chemical composition were most significant in salt content, but water and protein content were also affected. Water content decreased from the tail to head direction of the fillets, both in unsalted and light salted fillets. Changes in protein content were in opposite proportion to changes in water content. This could be seen in the fresh fish and also after freezing, both in unsalted and light salted fillets. Texture of the fish was not significantly affected by salting and freezing.     

柴油机高压共轨系统喷油量和喷油规律测量方法概述

对目前几种常用的测量柴油机高压共轨系统喷油量和喷油规律的方法、原理和装置进行了介绍与分析。     

复杂注水系统泵站优化调度的改进遗传算法

以耗电量最小为目标函数,排量、压力等限制为约束条件,建立了复杂注水系统泵站优化调度数学模型.针对系统运行特点,采用改进遗传算法对模型进行求解,同时优化出泵的运行状态和排量.改进遗传算法采用实数编码,并设计了随机多父辈交叉方法,改进了变异操作,操作过程中,给出了泵排量的处理方法,使各水量约束条件得到满足,大大减少了不可行解的产生,使算法的优化性能得到了提高.算例说明了该优化方法的有效性.     

单壁碳纳米管载嗜水气单胞菌外膜蛋白亚单位疫苗的研制及免疫效果评价

为了筛选细菌的疫苗候选蛋白,本实验根据课题组前期的研究,挑选了2个嗜水气单胞菌外膜蛋白(A0KLQ6和A0KH89),以A0KHR9(OmpAII)为对照,通过比较外膜蛋白与碳纳米管载外膜蛋白通过腹腔注射与浸泡免疫模式,比较和评价斑马鱼的免疫反应与免疫保护效果。结果显示,腹腔注射组和浸泡组与普通蛋白组对比,碳纳米管载蛋白能够显著提高斑马鱼免疫相关基因表达量。腹腔注射组在5μg剂量下免疫A0KHR9、A0KLQ6和A0KH89蛋白分别获得61.60%、 65.39%和84.96%相对免疫保护率(relative immune protection rate,RPS),在5μg剂量下免疫碳纳米管载蛋白SWCNTs-A0KHR9、SWCNTsA0KLQ6和SWCNTs-A0KH89分别获得68.10%、77.50%和90.43%的RPSs。浸泡组在40 mg/L剂量下免疫A0KHR9、A0KLQ6和A0KH89蛋白分别获得30.80%、25.85%和37.80%的RPSs,在40 mg/L最高剂量下免疫碳纳米管载蛋白疫苗SWCNTs-A0KHR9、SWCNTsA0KLQ6和SWCNTs-A0KH89分别获得63.60%、73.74%和68.49%的RPSs。研究表明,铁离子限制下嗜水气单胞菌外膜蛋白A0KLQ6和A0KH89等能够作为抗嗜水气单胞菌的亚单位疫苗候选蛋白,而单壁碳纳米管载蛋白通过注射免疫与浸泡免疫均能显著提升免疫疗效,是一种疫苗的适宜纳米载体。以上研究为寻找高效的嗜水气单胞菌亚单位疫苗候选蛋白及佐剂提供了理论依据。     

过量空气系数与废气再循环率耦合对汽油机性能的影响

为了提高汽油机部分负荷下的燃油经济性,该文通过改变废气再循环率(EGR,exhaust gas recirculation)和过量空气系数,在一台增压进气道喷射汽油机上进行试验,研究了过量空气系数与EGR耦合对发动机部分负荷下燃油经济性和排放的影响。结果表明:随着EGR率和过量空气系数α的增加,燃烧循环变动率变大,燃烧持续期变长,且EGR是影响两者的主要因素;泵气损失显著减少,有效燃油消耗率显著降低,与EGR率=0和过量空气系数α=1条件下相比,EGR率=20%和过量空气系数α=1.1条件下的有效燃油消耗率降低了8.37%;随着EGR率的增加,THC(total hydrocarbon)排放增加,但NOx排放显著降低,高达约80%。因此,将三效催化转化器和EGR技术相结合,燃烧稀混合气,可以在满足欧Ⅴ排放法规的同时,大幅提高汽油机部分负荷下的燃油经济性。     

根际注射施肥对黄土高原苹果氮素吸收利用及产量和品质的影响

【目的】在西北黄土高原地区,春季干旱少雨和肥料利用率低限制着生产优质苹果。国外通常应用水肥一体化技术来克服水肥利用率低的问题,但由于其硬件设施要求高,投资大,短期内在我国难以推广。近年来我国采用的根际注射施肥可利用施肥枪将肥料溶液直接注入根际土壤中,施肥成本低且技术简单。本研究利用同位素15N示踪技术,研究根际注射施肥对苹果氮素吸收利用及产量品质的影响,可为黄土高原果园水肥高效利用提供依据。【方法】以9年生富士/M26/新疆野苹果为试验材料,利用15N尿素标记肥料去向,最后通过MAT-251质谱计测15N丰度,得出果树各器官和土壤的肥料利用率。同时利用叶绿素仪(SPAD-502)测定标记叶片的SPAD值,用浸以磷酸甘油溶液的海绵进行田间原位测定,得到土壤氨挥发的量,用静态箱—气相色谱法测定土壤的N2O逸失量。综合对比分析黄土高原传统环状开沟撒施肥与根际注射施肥对苹果吸收利用氮素、肥料氮在土壤中残留及果实产量和品质的影响。【结果】黄土高原苹果园根际注射施肥的优越性体现在:1)施肥后一个月内,果园土壤的气态氮素损失发生变化,根际注射施肥比传统环状开沟施肥的氨挥发总量低54.9%,同时N2O的排放通量低5.0%。2)根际注射施肥后,促进了肥料在土壤中的扩散范围,扩大了根系肥水吸收容积,叶片和果实吸收的肥料氮比例(Ndff%)在整个生长季始终处于较高水平。生长季末期,根际注射施肥的整株氮素当季吸收率为53.04%,比环状开沟施肥提高12.25个百分点,表明根际注射施肥有利于氮素更快地被吸收利用,显著提高苹果树的氮素当季利用率。3)生长季末,在0—60 cm土层内,根际注射施肥的土壤氮素残留率为36.55%,而环状开沟施肥为43.13%,前者显著低于后者。4)在整个生长季内,根际注射施肥处理下的树体新梢叶片内叶绿素含量(SPAD)值一直高于环状开沟施肥。根际注射施肥能提高苹果单株产量和单果重,其单果重和单株产量分别比环状开沟施肥处理提高了3.8%和19.7%。【结论】黄土高原地区推广的果树根际注射施肥技术可以有效提高苹果树体氮素的利用率,降低了土壤中的氮素残留。此外注射施肥的深度、注射量、密度和时间均可根据不同时期的养分需要随时调整,使水肥在土壤中均匀分布,达到节水节肥的目的。同时可避免传统施肥时挖坑作业对浅土层吸收根的损伤,降低劳动力成本。综合来看,根际注射施肥是提高黄土高原区旱地苹果树肥水利用率、产量和品质的有效方式之一。     

Comparison of cavity modification and community involvement as strategies for hornbill conservation in Thailand

We monitored breeding success of hornbills in Thailand for four sympatric species at Khao Yai National Park during 1981-2002 and six species at Budo-Sungai Padi National Park during 1994-2002. Within a 60 km² study area at Khao Yai, the well protected area, use of available cavities ranged from 94% in 1984 to 50% in 1993. Competition for nesting cavities was 40% of cavities available indicating the shortage of suitable cavity. We monitored the cavity condition and modified 48 cavities and, between 1996 and 2000, these contributed annually 23-45% of nestings that were successfully fledged (n = 352). Within the 90 km² at Budo Mountain, with heavy human disturbance, use of available cavities for nesting decreased from 81% in 1997 to 30% by 2001 and of successful fledging from 96% in 1997 to 55% in 1999, but both increased once our involvement with local communities had eradicated poaching and reduced disturbance. Competition for nesting cavities here was 26% of cavities available indicating the effect of poaching. Both strategies were successful in the short term but long-term management of cavities in large forest trees is required at Khao Yai and of forest disturbance at Budo.