农艺性状优良冬小麦ph1b系的创造及标记辅助选择的应用

利用改进的桥梁亲本法, 即以阿勃5B缺体为桥梁亲本, 分别与受体冬小麦推广品种(农大95、 京411和京冬8号)和中国春ph1b突变体杂交, 两个组合F1(即5BPh1和5Bph1b单体)再杂交, 后代选择5Bph1b单体与受体亲本5BPh1单体杂交, 仅3个世代就获得了3个冬小麦ph1b中间育种系, 在杂交转育过程中, 我们用先前获得的3个SCAR标记对5     

假单胞杆菌B301对稻瘟病菌的拮抗性

B301是一株分离自空气中的假单胞杆菌（Pseudomonas sp.)。室内测定表明,它能抑制稻瘟病菌的分生孢子萌发和菌丝生长。病菌分生孢子在B301发酵液的2倍稀释液中完全不能萌发,40倍稀释液中的萌发率为35.58%,比对照下降59.4%。B301发酵液稀释140倍时,对病菌菌丝生长的抑制率为52.1%,其抑制菌丝生长的最大稀释度为4480倍。B301发酵液中的抗菌物质具有较强的耐热性,在80℃下,60min仍保持原有活性,但当温度上升到100℃后则不稳定,10min内活性显著下降,80min后完全失活。田间防治试验表明,B301发酵液防治稻瘟病的效果为45％左右,与对照药剂20％三环唑可湿性粉剂500倍液防效相当。此外,B301还能有效抑制棉花炭疽病菌和玉米小斑病菌的生长。     

The effects of dietary n-3 vs. n-6 fatty acids on ex-vivo LTB4 generation by canine neutrophils

Dietary n-3 polyunsaturated fatty acids are widely used for amelioration of inflammatory skin disease in dogs. In this study, a diet containing two different sources of n-3 polyunsaturated fatty acid–triglyceride (from menhaden oil) and concentrated ethyl esters–was fed to one group of six purpose-bred dogs, while an isocaloric isonitrogenous diet with corn oil (n-6 polyunsaturated fatty acids) was fed to another group of eight purpose-bred dogs for six weeks. Peripheral blood neutrophils, isolated at week–1 (baseline), week 2 and week 6, were stimulated with calcium ionophore A23187 and the amount of leukotriene B₄ produced was determined via reversed-phase high performance liquid chromatography. Analysis of variance of log-transformed data revealed a significant effect for diet ( P  = 0.005) at six weeks, with dogs fed the high n-3 polyunsaturated fatty acid diet having significantly less mean ex vivo neutrophil leukotriene B₄ production than dogs fed the high n-6 polyunsaturated fatty acid diet. Further studies on the clinical usefulness of n-3 polyunsaturated fatty acid ethyl esters are warranted.     

野桑蚕CYP305B1V1基因的组织表达特异性初探

抽提不同处理的野桑蚕各组织总mRNA,利用地高辛标记的野桑蚕CYP30581V1的cDNA基因作为探针,通过点杂交方 法研究野桑蚕CYP3051B1基因的组织表达特异性。实验结果显示,CYP30581V1基因在氟化物诱导后的野桑蚕中肠、脂肪体、马氏管、体壁 中有特异性转录。     

B型烟粉虱若虫龄期和虫数与西葫芦银叶率的关系

通过B型烟粉虱虫龄、虫数和不同西葫芦品种对西葫芦银叶表达影响的研究表明:2头以上的2龄若虫刺吸危害,西葫芦就可以出现银叶症状.在进行试验的6个西葫芦品种中,金玉对B型烟粉虱的危害表现出良好的抗性.     

胰岛激活蛋白B聚体的各亚基克隆及表达的初步研究

研究将百日咳杆菌用B-G培养基活化后再在改良的S-S培养基上液体培养,用CTAB-NaCl法提取百日咳杆菌的基因组DNA.经PCR扩增出胰岛激活蛋白 B聚体的四个亚基基因,分别将其克隆于pGEX-6p-1载体上,并探讨了在大肠杆菌BL21(DE3)中可溶性表达IAP B聚体各亚基的可能性.通过控制诱导表达温度,S2,S3均可以实现可溶表达,以GST亲和层析得到的S2终产物0.4 mg/L培养物, S3为0.6 mg/L培养物.S4表达虽是可溶性的,但由于融合蛋白的等电点近于7,多步纯化易损失.S5的表达量太低,有待进一步分析.     

A new 2-arylbenzofuran from the root bark of Chinese Morus cathayana

A new 2-arylbenzofuran, sanggenofuran B (3',5'-dihydroxy-6-methoxy-4'-prenyl-2-arylbenzofuran), from the root bark of Chinese Morus cathayana is reported.     

Plant-derived natural products exhibiting activity against formosan subterranean termites (Coptotermes formosanus)

The Formosan subterranean termite, Coptotermes formosanus Shiraki, is among the most devastating termite pests. Natural products derived from plant extracts were tested in a discovery programme for effective, environmentally friendly termite control agents. Among the natural products tested, vulgarone B (isolated from Artemisia douglasiana Besser), apiol (isolated from Ligusticum hultenii (Fern.) Calder & Taylor) and cnicin (isolated from Centaurea maculosa Lam.) exhibited significantly higher mortalities than in untreated controls in laboratory bioassay. These compounds are present at high levels in their respective plant sources and also possess other biological activities such as phytotoxic and antifungal properties.     

芥菜型油菜×羽衣甘蓝种间杂种的获得及其性状表现

芸薹属内遗传和变异类型极其丰富,这为芸薹属植物种间杂交提供了条件。通过甘蓝(B. oleracea,2n=CC=18)和芥菜型油菜（2n=AABB=36）的种间杂交可以获得六倍体新物种,为油菜育种创造新的种质资源。本文选用芥菜型油菜和黄籽羽衣甘蓝(B. oleracea var. aceaphala,2n=CC=18)进行种间杂交,在10个杂交组合中,共授粉559朵花,     

The Pseudomonas aeruginosa HSP70-like protein DnaK induces IL-1β expression via TLR4-dependent activation of the NF-κB and JNK signaling pathways

IL-1β expression is increased in response to P. aeruginosa infection, but the responsible proteins have not been clearly elucidated. Here, we demonstrate for the first time that IL-1β expression is induced in response to the heat shock protein 70-like protein DnaK. Treatment with recombinant DnaK (rDnaK) increased IL-1β expression in a dose- and time-dependent manner, and the release of mature IL-1β in response to rDnaK was detected to an extent similar to that stimulated by the well-known agonists, lipopolysaccharide and nigericin. rDnaK-mediated IL-1β expression was driven by the NF-κB signaling pathway. In addition, expression was controlled by the JNK signaling pathway, although these two signaling cascades act independently upon rDnaK stimulation. Finally, rDnaK-induced IL-1β expression was initiated via the action of TLR4. Taken together, the data reveal that P. aeruginosa-derived DnaK induces expression of IL-1β via TLR4-dependent activation of the NF-κB and JNK signaling pathways.