Effects of Wnt10b on dermal papilla cells via the canonical Wnt/β‐catenin signalling pathway in the Angora rabbit

Wnt10b is a member of Wnt family that plays a variety of roles in biological functions, including those in the development of hair follicles. To investigate the effect of Wnt10b on hair growth in the Angora rabbit and to determine the underlying molecular mechanism, we cultured dermal papilla (DP) cells with exogenous Wnt10b in vitro. We observed the expressions of downstream critical gene β‐catenin and lymphoid enhancer‐binding factor 1 (LEF1) in Wnt/β‐catenin pathway. The levels of β‐catenin mRNA and protein were higher in the Wnt10b group of DP cells than in the Control group, and the mRNA level of LEF1 in the Wnt10b group was higher than in the Control group. Moreover, translocation of β‐catenin from cytoplasm to nucleus was activated in the Wnt10b group. Furthermore, the mRNA levels of the hair follicle‐regulatory genes, insulin‐like growth factor‐1 (IGF‐1) and alkaline phosphatase (ALP), and the protein activity of ALP was also upregulated in the Wnt10b group compared to their corresponding levels in the Control group. These data suggest that Wnt10b could activate the canonical Wnt/β‐catenin signalling pathway to induce DP cells in the Angora rabbit. In addition, the proliferation of DP cells was significantly promoted when cultured with Wnt10b for 48 and 72 hr, suggesting that Wnt10b plays a pivotal role in the proliferation and maintenance of DP cells in vitro. In conclusion, this study demonstrates that Wnt10b may promote hair follicle growth in Angora rabbit through the canonical Wnt/β‐catenin signalling pathway that promotes the proliferation of DP cells.     

Effects of elevated CO2 concentration on the respiratory behavior of the branches of field-grown hinoki cypress (Chamaecyparis obtusa)

The effects of elevated atmospheric CO₂ concentrations on the nighttime respiration were examined for two sample branches of a hinoki cypress tree (Chamaecyparis obtusa) growing in the field with an open gas exchange system for a one-year period from July 1994 to June 1995. The branches were of a similar size and located at a similar position within the crown. One branch was subjected to an elevated CO₂ concentration of 800 μmol mol⁻¹ and the other was subjected to ambient air which had a CO₂ concentration of about 370 μmol mol⁻¹. Nighttime respiration rate was higher in elevated CO₂ level than in ambient CO₂ level. The relationship between nighttime respiration and the corresponding nighttime air temperature was fitted by the exponential function in every month of the year. The segregation of regression lines between the two CO₂ treatments increased gradually as the seasons progressed during the treatment period. TheQ ₁₀ values for nighttime respiration were lower in elevated CO₂ (1.9 ≤Q ₁₀ ≤ 3.7) than in ambient CO₂ (2.4 ≤Q ₁₀ ≤ 4.5) in every month of the year. TheQ ₁₀ was inversely related to the monthly mean nighttime air temperature in both elevated and ambient CO₂. The estimated daily nighttime respiration rate under both CO₂ treatments had a similar seasonal pattern, which almost synchronized with the temperature change. The respiration ratio of elevated CO₂ to ambient CO₂ increased gradually from 1.1 to 1.6 until the end of the experiment. Our results indicate that the CO₂ level and the temperature have a strong interactive effect on respiration and suggest that a potential increase in respiration of branches will occur when ambient CO₂ increases.     

经济供电半径的实用计算方法与分析

基于满足负荷要求及各项技术指标的条件下,以供电范围内单位面积所承担的总费用最小为原则,提出了东北地区６６／１０ｋＶ供电方式的１０ｋＶ配电线路经济供电半径的实用计算方法。同时分析了常规变电站和小型化变电站的合理供电半径,并给出了供电半径与负荷密度之间的函数关系。     

Production profile and comparison analysis of main toxin components of Fusarium oxysporum f. sp. sesami isolates with different pathogenicity levels

Fusarium wilt is a common fungal disease in sesame caused by Fusarium oxysporum f. sp. sesami (FOS). To determine the toxin production profiles of the FOS isolates with different pathogenicity levels under various culture conditions, we assessed the content variation of fusaric acid (FA) and 9, 10-dehydrofusaric acid (9, 10-DFA) produced by the four representative FOS isolates. Results indicated that the concentration of FA reached to a maximum of 2848.66 ​μg/mL in Czapek medium, while 9, 10-DFA was mainly produced in Richard and Low-carbon Richard medium. The concentration of 9, 10-DFA on Richard culture medium varied from 0 ​μg/mL to 716.89 ​μg/mL. Of the five culture media used in this study, Czapek culture medium was the most conductive to produce FA. FA production was significantly affected by culture medium, culture time, and their interactions. Results suggest that there is no correlation between toxin production and pathogenicity level of FOS isolates. These findings provide key information for the mechanism analysis of FOS- sesame interaction and pathogen control.     

BnaYUCCA10基因在不同油酸含量油菜中的表达

为明晰油菜BnaYUCCA10基因与油酸含量之间的关系,以miRNA测序得到的BnaYUCCA10基因为对象,采用实时荧光定量PCR(RT–PCR)技术研究BnaYUCCA10基因在30份不同油酸含量的甘蓝型油菜不同部位和生长时期的表达情况,并对BnaYUCCA10基因是否参与油酸的代谢进行分析.结果表明:营养生长期大...     

夜间增温与铜污染对麦田土壤无机氮库的影响

基于夜间增温（设增温、常温两个水平）与铜污染 [设CK（6 mg·kg^-1）、LP(43 mg·kg^-1）、MP（155 mg·kg^-1）、SP（209 mg·kg^-1）4个梯度] 复合因素的7 a旱地定位试验,结合室内20℃和30℃下恒温培养91 d,分析了麦田土壤硝态氮、铵态氮和无机态氮含量的变化及其稳定特征。研究结果表明：土壤硝态氮、铵态氮和无机态氮随着土壤中铜含量的增加均表现为先增后降趋势,夜间增温下CK土壤硝态氮和无机态氮含量分别显著降低10.8%和5.7%,在LP、MP处理下硝态氮含量分别显著下降17.4%、15.2%,但在SP处理下土壤硝态氮、铵态氮分别显著增加6.9%和15.1%,增温与铜含量对土壤无机态氮和硝态氮的影响呈现显著的交互效应。20℃和30℃恒温培养使无机态氮含量增加1.6~4.6倍、铵态氮含量增加5.2~13.3倍,却使硝态氮含量减少40.3%~65.1%,20℃比30℃恒温培养后土壤中有较高的无机态氮、铵态氮含量和铵/硝比。夜间增温使土壤净氮矿化作用、氨化作用的Q₁₀值均有所提高,但硝化作用的Q₁₀值显著降低16.8%,适当的铜含量（LP：43 mg·kg^-1）将加快土壤氮的氨化、矿化和硝化速率。因此,夜间增温提高了旱地麦田土壤氮矿化作用温度敏感性,降低了无机态氮库稳定性,土壤铜污染与土壤氮库转化和增温响应的关系具有浓度效应。     

哈尔滨沙尘天气成因及其对城市大气污染的影响

文章从空气动力学和天气学角度出发探讨了大风、对流层低层的强烈垂直不稳定及高、低空急流对哈尔滨沙尘天气做出的贡献,分析了沙尘天气时城市大气中几种主要污染物变化情况。研究表明,哈尔滨的沙尘天气主要来源是内蒙古中部的沙地,但近年也常出现本地沙源引起的沙尘天气;沙尘天气期间,城市大气中的可吸入颗粒物浓度出现异常,其浓度变化和沙尘天气具有同步性特征;沙尘天气期间,SO2含量呈明显减弱趋势;NO2污染物的浓度变化则表现较平稳。     

PRRSV特异性肽对CSF、PRRS免疫猪IFN-Y、IL-10分泌的影晌

应用PRRSV-GP5特异性肽刺激CSF、PRRS免疫猪外周血淋巴细胞,采用EI。IsA方法测定不同时间点淋巴细胞分泌IFN-7及IL-10的水平,分析PRRSv_GP5特异性肽刺激后,淋巴细胞培养上清中细胞因子的变化规律。结果显示,CSFV高抗PRRSV低抗体组于PRRSV免疫14d,肽刺激组中IFN-7水平显著高于对照组（P〈0．05）;其他组均差异不显著（P〉0．05）。PRRSV免疫14d,细胞培养24,72h后加PRRSV—GP5肽刺激,各组中试验组与对照组相比IL-10水平明显降低（P〈0．01）;PRRSV免疫28d,各组中肽刺激组与空白组相比,IL-10水平均无显著性差异（P〉0．05）。结果表明,PRRSV-GP5肽在PRRSV免疫早期可以促进IFN-7的分泌,并抑制IL-10的产生,但在免疫后期对淋巴细胞分泌的调节作用还需进-步研究。