波尔山羊瘤胃积沙的诊治

蓬莱市潮水镇某养羊专业户从外地购进种波尔山羊 1只。因吞食大量泥沙导致瘤胃积沙 ,经 5 d诊治 ,排除泥沙 ,病羊康复。现将诊治情况介绍如下。1　临床症状病羊远离食槽 ,不吃草 ,不反刍 ,鼻镜时干时湿 ,神情不安 ,目光呆痴 ,左肷窝外胀满 ,屡作排粪姿势 ,但不见粪便排出或偶尔     

Pathogen Analysis of Bovine Respiratory Disease Complex

To investigate the pathogen of bovine respiratory disease complex (BRDC) of a dairy farm in Guangxi, a strain of Mycoplasma and a strain of gram-negative pathogenic bacterium were isolated and identified by the means of field surveys, clinic observation, pathological examination, isolation studies and so on.Treatments were taken according to drug sensitivity test results.The Mycoplasma strain, growing on PPLO medium, formed typical "fried egg" colonies.A 448 bp of oppF fragment was amplified by PCR from the strain and had 98.4% nucleotide identity with Mycoplasma bovis reference isolate PG5 of USA.The biochemical features of the gram-negative bacterial isolate were same with Serratia marcescens.The PCR amplified 16S rDNA of the gram-negative pathogenic bacterium strain was 1 400 bp.It shared 99.0% nucleotide identity with other Serratia marcescens reference strains obtained from GenBank.Animal experiment showed that the gram-negative pathogenic bacterium isolate could cause the mice to die.The drug sensitivity tests showed all isolates were sensitive to spectinomycin, azithromycin, amikacin, gentamicin and neomycin.It was effective to treat with dexamethasone and spectinomycin.Pathogen analysis and drug treatment showed that the BRDC was caused by Mycoplasma bovis and Serratia marcescens.     

Isolation,Identification and Genetic Evolution Analysis of Mink Serratia marcescens

In order to determine drug resistance, pathogenicity and genetic of the pathogenic bacteria in mink of Heilongjiang province, three strains were isolated from dead minks and identified. Based on colony morphology, microscopic characteristics, biochemical test and 16S rRNA sequencing. The antibiotic susceptibility to 24 antibiotics were detected by using standard K-B paper method, and virulence assay, phylogenetic analysis were carried out at the same time. The result showed that the isolates were highly tolerance to antibacterials, could cause 100% death of mice in experimental group and were closely related to strains from activated sludge and volcanic deposits. It showed that Serratia marcescens infection of mink existed in Heilongjiang province. Strains resistance phenomenon was serious and had a strong pathogenicity, and might be originated from the environment. It was the first time that the pathogenic Serratia marcescens isolated from the mink was reported. The results provided information for the detection, identification, diagnosis, treatment and prevention of related diseases.     

模拟干旱和盐胁迫下沙冬青种子硬实特性和抗逆性的研究

将1~5 d内每天吸胀的沙冬青(Ammopiptanthus mongolicus)种子视为不同硬实程度的种子T₁,T₂,T₃,T₄和T₅,与第5 d仍未吸胀的硬实种子(记作T_r)作为试材对其硬实特性和抗逆性进行研究,以期为沙冬青种子栽培驯化提供理论依据。结果表明:随着硬实程度的提高,种子的发芽势、发芽指数、活力指数呈上升趋势;电导率和丙二醛(MDA)含量逐渐下降;可溶性蛋白含量逐渐上升。不同处理对种子的硬实破除效果表明,80℃热水浸泡种子可达到最佳破除效果。通过测定模拟干旱胁迫和盐胁迫下不同硬实程度种子的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和脯氨酸(Pro)含量,表明在相同逆境条件下,随硬实程度的加深,除极个别外,各项指标值均有不同程度的升高,且一定胁迫强度下硬实种子萌发幼苗的各酶活性和脯氨酸含量均极显著高于其他萌发幼苗(P<0.01),因此,硬实种子比非硬实种子有较强的保护酶调节系统。硬实程度越高,耐旱和耐盐能力也随之升高。     

风洞模拟“攀援植物-围栏”复合沙障不同疏透度下的防风效果

为探索受风蚀影响围封区域的新型治理技术措施,本研究对不同疏透度(β)“攀援植物-围栏”复合沙障的防风作用进行了风洞模拟试验。分别在6、8和10 m·s^-1净风条件下对模型A (网围栏,β:96%～98%)与4种复合沙障[模型B (无叶期,β:59%～64%)、模型C (无叶期,β:50%～55%)、模型D (有叶期,β:40%～45%)、模型E (有叶期,β:30%～35%)]进行试验。结果表明：1)不同类型复合沙障流场结构均呈“减-增-减”的变化趋势,障体背风侧存在风影减速区,网围栏前后流场结构一致,不存在减速区。2)不同风速条件下,无叶期复合沙障(模型B、模型C)在10 m·s^-1风速下近地表平均防风效能最低,分别为18.55%与18.38%,有叶期复合沙障(模型D、模型E)则在6 m·s^-1风速下最低,分别为44.03%与45.42%,有叶期复合沙障防风效应随风速的增大而增强,无叶期复合沙障则相反。3)综合分析表明,当障体疏透度为59%～64%时便可产生防风效果,复合沙障防风效果优于网围栏,障体有叶期防风效果优于无...     

湘沙猪配套系母系日粮粗蛋白质需要量研究

选择体重35 kg左右的湘沙猪配套系母系猪48头,随机分成4组,每组2个重复,每重复6头猪,各重复公母比例一致,4个处理分别饲喂4种不同蛋白水平的日粮,试验期104 d。结果表明：日粮不同蛋白水平对湘沙猪配套系母系猪的日增重、料重比、胴体品质等育肥性能指标无明显影响。综合分析认为,湘沙猪配套系母系猪35～60 kg和65～100 kg阶段可采用低蛋白水平日粮,适宜蛋白质需要量分别为14.03%和13.03%。     

东阿拉善不同发育阶段沙冬青种群的空间分布格局

为了揭示国家二级保护植物沙冬青(Ammopiptanthus mongolicus)种群在发展过程中的空间分布格局及变化规律,本研究选择东阿拉善荒漠的沙冬青作为研究对象,采用大小结构法将沙冬青植株分为3个等级,运用成对相关函数g(r)对3个发育阶段沙冬青种群进行分析。结果表明：年轻的增长型、成熟的稳定型和衰退的下降型种群在空间上交错分布。在增长型和稳定型种群中,沙冬青幼龄(Ⅰ级)个体呈现小尺度聚集,中、大尺度随机分布,中龄(Ⅱ级)个体呈现小尺度均匀分布,中尺度聚集性增加,大尺度随机分布。从稳定型到下降型种群,沙冬青老龄个体(Ⅲ级)在小尺度上均匀分布加大,其他尺度以随机分布为主。沙冬青在中、小尺度上Ⅰ级与Ⅱ级植株存在正关联,小尺度上Ⅲ级植株对另两个级的植株存在显著负关联。研究结果为该种的保育提供理论依据。     

行距和播量对沙芦草种子产量及其构成因子的影响

为探究干旱半干旱区沙芦草(Agropyron mongolicum)种子生产适宜的行距和播量配比,本试验采用双因素裂区试验设计,分别设4个行距水平(20,30,40,50 cm)和4个播量水平(10,15,20,25 kg·hm^-2),研究了不同行距和播量配比对沙芦草种子产量及产量构成因子的影响。结果表明,行距对沙芦草实际种子产量和理论种子产量有极显著影响(P<0.01),播量对沙芦草实际种子产量有显著影响(P<0.05),行距和播量对沙芦草实际种子产量有极显著的正交互作用(P<0.01)。通径分析表明,小穗数、小花数、种子数、穗长和生殖枝数对种子产量的影响具有直接作用,对种子产量贡献最大的是生殖枝数;回归寻优模型分析得出,当行距为20 cm,播量为15 kg·hm^-2时实际种子产量最高。因此,在宁夏中部干旱区沙芦草种子生产中建议种植密度应为行距为20 cm,播量为15 kg·hm^-2最佳。     

克痢沙防治猪鸡细菌性泻痢试验研究

由致病性大肠杆菌、沙门氏菌引起的猪、鸡的腹泻病是造成集约饲养场巨大的经济损失的原因之一.为寻求较好的防制方法,作者对其做了一些研究,现报告于后.