甘南州玛曲县棘珠蚴病流行病学调查

通过对玛曲县欧拉、尼玛等乡牧场棘球蚴病高发区牛15头、羊100只、犬15条剖检和分析发现，棘球蚴感染率牦牛为66．67％、羊为50％、母羊为75％，母犬细粒棘球绦虫的感染率为27％、人的棘球蚴感染率为0．3％。对1～5岁绵羊各50只，进行的间接血凝感染抗体检测表明：1岁羊血清滴度较低，2岁和4岁羊的滴度较高，而3岁和5岁羊的滴度略低于前一年龄组。酶联免疫吸附试验结果：1岁羊血清平均值最低，2岁最高，以后各年龄组略微降低，并大致保持平稳状态。     

手术摘除羊牙龈棘球蚴一例

棘球蚴病是由细粒棘球绦虫蚴寄生于动物肝、肺等脏器所引起的疾病。笔者于2003年5月收治一例波尔山羊牙龈棘球蚴病。经手术摘除获得了较好疗效。     

鹅卷棘口吸虫病的诊治

2005年10月我市动物疾病防控中心门诊诊治了一起以盲肠坏死、出血、腹泻为特征的鹅病例。经临床剖检发现直肠内有淡红色的虫体，疑为吸虫，后经寄生虫检验室做病原学检查，诊断为鹅卷棘口吸虫。现将诊治情况报告如下：     

Construction of Full-length cDNA of Recombinant Rabies Virus SRV9 Strain Expressing EgM123 Gene of Echinococcus granulosus

The aim of the experiment was to construct the recombinant rabies virus SRV₉ vaccine strain with EgM123 gene by reverse genetics technology and provide the technical means for effective prevention and control of rabies and hydatidosis in China's agricultural and pastoral areas.In this study,the structural protein N,P and L genes of rabies virus SRV₉ were synthesized using gene synthesis technology,which was based on the complete genome sequence of rabies virus SRV₉ and the fusion fragment of the N-P-M fusion fragment and the rabies G gene,through the carrier of enzyme insertion connection methods,the recombinant rabies virus L gene,N-P-M gene fusion fragment and G+EgM123+eGFP gene fusion fragment were successively recombined on the expression vector pcDNA3.1(-) to construct the full-length cDNA of recombinant rabies virus SRV₉ with EgM123 gene.The synthesized genes were constructed on pcDNA3.1(-) expression vector,and the results of transformation,plasmid digestion and gene sequencing showed that the length of N,P,L,N+P+M and G+EgM123+eGFP gene fragments were 1 365,1 107,6 471,3 160 and 3 256 bp,respectively.The full-length cDNA fragment of EgM123 gene recombinant rabies virus full-length cDNA was 12 465 bp,and the sequencing results of each gene fragment were 100%.In this experiment,the full-length cDNA fragment of recombinant EgM123 rabies and eukaryotic expression vectors of the N,P and L genes of rabies virus were successfully constructed,which could save EgM123 gene recombinant rabies by reverse genetics,it also provided the reference for the development of rabies and hydatid disease combined gene recombinant oral live vaccine.     

饲料中可添加的几种物质

(1)抗球虫药类:氨丙啉、硝酸二甲硫胺、氯羟吡啶、苄氧喹甲酯、尼卜巴嗪、氢溴酸常山酮、氯苯胍、二硝托胺、沙拉洛西钠、莫能菌素、盐霉素、马杜拉霉素。(2)驱虫药类:越霉素A、潮霉素B。     

散养鸡棘口吸虫病的诊治

1发病情况莱州市大原镇李某,家有10亩荒地,散养1200只公鸡,120日龄,疫苗按计划防疫,平均体重1.75kg。近期大群精神沉郁,食欲下降,粪便稀薄,消瘦并陆续死亡。2005年8月26日带2只病鸡到我站就诊。2剖检症状回肠与直肠交接处发现虫体,头入肠壁,淡粉红色长1.5mm左右。肠粘膜肥厚,有     

基于颠茄发根的外源基因表达系统的建立

 用携带植物高效表达载体pCAMBIA1304 ⁺ “解除武装”的重组C58C1工程菌转化颠茄无菌苗真叶, 发根诱导率达100%。PCR检测证实发根型质粒pRiA4和表达型质粒pCAMBIA1304 ⁺均能整合到颠茄发根基因组内, 共转化率达30%。建立了基于颠茄发根的高效外源基因表达系统, 为将托品烷类生物碱东莨菪碱的生物合成关键酶基因导入颠茄, 实现其次生代谢工程及开展分子育种奠定了基础。     

“棘防E号”诱发小白鼠骨髓细胞的微核效应

采用微核试验观察“棘防E号”对小鼠骨髓细胞遗传毒性作用和量效关系。结果表明,“棘防E号”剂量为1/2 LD50(1 404 mg/kg)、1/4LD50(702 mg/kg)、1/8 LD50(352 mg/kg)时微核率分别为(140.27±4.71)%、(64.04±3.07)%、(42.01±1.99)%。其中1/2 LD50(1 404mg/kg)剂量组的微核率是阴性对照组的3.2倍,说明“棘防E号”的剂量超过1/2 LD50以上时对小白鼠骨髓细胞有一定的致突变作用。     

疯草-内生真菌共生体的研究与展望

就近年来国外对疯草内生真菌的分离、宿主植物体内生真菌分布、疯草苦马豆素种群分布特征及其与内生真菌的相关性研究进展作一综述,以期更好地利用疯草及内生真菌造福人类。     

棘球蚴病的检疫与处理

棘球蚴病（又称包虫病）。是寄生于人和猪、牛、羊等哺乳动物内脏及肌肉内所引起的一类寄生虫病。在我国引起动物和人棘球蚴病的病原为棘球蚴。它不仅压迫组织器官造成其严重变形．而且由于囊泡破裂．囊液可导致再感染或过敏性疾患。对人畜造成危害。