黄花棘豆对小白鼠某些免疫功能的影响

通过T淋巴细胞值、小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬百分比和吞噬指数计数,探讨了黄花棘豆对小鼠某些免疫功能的影响。结果表明,实验组小鼠的T淋巴细胞值、吞噬百分比和吞噬指数明显低于对照组(P<0.001),初步说明黄花棘豆对小鼠免疫水平有抑制作用。     

土壤种子库中棘豆属种子的密度、寿命和活力测定

在青海湖地区的不同样地上,对甘肃棘豆、宽苞棘豆和黄花棘豆3种棘豆土壤种子库的密度进行了测定,种子密度随着植被中种群分盖度的大小而变化幅度很大,每平方80～2 220粒;同时采用四脞法对土壤种子库中的棘豆种子进行种子活力测定,其中77%的种子具有活力;用尼龙袋法测定甘肃棘豆种子在土壤种子库中的寿命,5年后埋置在土壤环境中的甘肃棘豆种子有50%的还存在,其中5%的种子具有活力;暴露在土壤表面环境中的种子有33%的种子存在;5年期间,埋置的种子和暴露在土壤表面的种子每年分别约有5%和14%的种子在发芽。     

甘南州玛曲县棘珠蚴病流行病学调查

通过对玛曲县欧拉、尼玛等乡牧场棘球蚴病高发区牛15头、羊100只、犬15条剖检和分析发现，棘球蚴感染率牦牛为66．67％、羊为50％、母羊为75％，母犬细粒棘球绦虫的感染率为27％、人的棘球蚴感染率为0．3％。对1～5岁绵羊各50只，进行的间接血凝感染抗体检测表明：1岁羊血清滴度较低，2岁和4岁羊的滴度较高，而3岁和5岁羊的滴度略低于前一年龄组。酶联免疫吸附试验结果：1岁羊血清平均值最低，2岁最高，以后各年龄组略微降低，并大致保持平稳状态。     

手术摘除羊牙龈棘球蚴一例

棘球蚴病是由细粒棘球绦虫蚴寄生于动物肝、肺等脏器所引起的疾病。笔者于2003年5月收治一例波尔山羊牙龈棘球蚴病。经手术摘除获得了较好疗效。     

Construction of Full-length cDNA of Recombinant Rabies Virus SRV9 Strain Expressing EgM123 Gene of Echinococcus granulosus

The aim of the experiment was to construct the recombinant rabies virus SRV₉ vaccine strain with EgM123 gene by reverse genetics technology and provide the technical means for effective prevention and control of rabies and hydatidosis in China's agricultural and pastoral areas.In this study,the structural protein N,P and L genes of rabies virus SRV₉ were synthesized using gene synthesis technology,which was based on the complete genome sequence of rabies virus SRV₉ and the fusion fragment of the N-P-M fusion fragment and the rabies G gene,through the carrier of enzyme insertion connection methods,the recombinant rabies virus L gene,N-P-M gene fusion fragment and G+EgM123+eGFP gene fusion fragment were successively recombined on the expression vector pcDNA3.1(-) to construct the full-length cDNA of recombinant rabies virus SRV₉ with EgM123 gene.The synthesized genes were constructed on pcDNA3.1(-) expression vector,and the results of transformation,plasmid digestion and gene sequencing showed that the length of N,P,L,N+P+M and G+EgM123+eGFP gene fragments were 1 365,1 107,6 471,3 160 and 3 256 bp,respectively.The full-length cDNA fragment of EgM123 gene recombinant rabies virus full-length cDNA was 12 465 bp,and the sequencing results of each gene fragment were 100%.In this experiment,the full-length cDNA fragment of recombinant EgM123 rabies and eukaryotic expression vectors of the N,P and L genes of rabies virus were successfully constructed,which could save EgM123 gene recombinant rabies by reverse genetics,it also provided the reference for the development of rabies and hydatid disease combined gene recombinant oral live vaccine.     

饲料中可添加的几种物质

(1)抗球虫药类:氨丙啉、硝酸二甲硫胺、氯羟吡啶、苄氧喹甲酯、尼卜巴嗪、氢溴酸常山酮、氯苯胍、二硝托胺、沙拉洛西钠、莫能菌素、盐霉素、马杜拉霉素。(2)驱虫药类:越霉素A、潮霉素B。     

散养鸡棘口吸虫病的诊治

1发病情况莱州市大原镇李某,家有10亩荒地,散养1200只公鸡,120日龄,疫苗按计划防疫,平均体重1.75kg。近期大群精神沉郁,食欲下降,粪便稀薄,消瘦并陆续死亡。2005年8月26日带2只病鸡到我站就诊。2剖检症状回肠与直肠交接处发现虫体,头入肠壁,淡粉红色长1.5mm左右。肠粘膜肥厚,有