Pathogenic mechanisms of caprine arthritis-encephalitis virus

Goats infected with caprine arthritis-encephalitis virus (CAEV) show chronic arthritis and cachexia, which are progressive in nature. The immunopathogenic mechanisms responsible for these progressive clinical symptoms have not been fully elucidated. Various haematological and immunological parameters were evaluated in experimentally-infected goats showing typical signs of CAEV-induced disease. Infected goats showed recurrent lymphocytosis that may be due to constant presentation of antigen by infected cells of a monocyte/macrophage lineage. The serum alkaline phosphatase and -glutamyl transferase concentrations were elevated in infected goats, a characteristic of hepatic and bone disorders. All other serum chemistry parameters were similar between infected and control goats. Importantly, the serum tumour necrosis factor- (TNF-) levels were higher in infected goats. The cachexia seen in infected goats may be at least partly due to altered metabolism as a result of prolonged elevation of serum TNF- levels. Depressed natural killer cell activity was observed in infected goats and may contribute towards the establishment of a persistent infection with CAEV.Abbreviations AIDS acquired immunodeficiency syndrome - CAEV caprine arthritis-encephalitis virus - GGT -glutamyl transferase - HBSS Hanks' balanced salt solution - HIV human immunodeficiency virus - NK natural killer - PBMC peripheral blood mononuclear cells - PCR polymerase chain reaction - SAP serum alkaline phosphatase - TNF tumour necrosis factor     

Inhibition of K562 cell growth by antisense drug targeting with VEGF mRNA in vitro

AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs.     

试论高品质居住小区景观设计的基本理念和风格

通过对高品质居住小区特征与内涵的分析,提出了"以人为本"是"高品质小区"的基本设计理念以及用"小区自己的方式"来设计才能真正体现高品质小区的设计风格的观点.以此构建集人性化、休闲化、个性化等特征于一体的生态环保小区、文化小区.     

土壤微塑料影响植物生长的因素与机制研究进展

土壤中的微塑料可通过多种方式影响植物生长,并且其在植物体内积累会最终通过食物链进入人体,厘清微塑料对植物生长的影响及机制,有助于系统掌握其在土壤-植物体系中的环境行为。微塑料的赋存状态和理化特征均可影响其对植物的作用效果,本文从粒径、形状、浓度、种类、塑料添加剂和老化程度等方面,梳理了土壤微塑料影响植物生长的主要因素及作用机制,并对未来研究的重点内容提出展望,以期为进一步明晰微塑料对土壤生态系统的影响提供参考。     

城市夜间形象初探

从城市规划和建筑学的角度研究城市夜间形象;结合形态美学、行为心理学、环境心理学等学科进行研究,将安全感、视觉舒适性、视觉趣味、活动激发性以及可识别性作为夜间形象评价指标并分别进行具体地分析。     

桂花在园林中的审美艺术与造景

在我国的园林植物中,桂花因其优美的姿态与飘逸的幽香而成为一种传统的造景植物。由于桂花在姿态、文化、景观等方面的审美功能,被广泛应用于植物造景中来表现其丰富多采的园林美感及深厚的文化蕴涵。桂花造景主要有独立造景、与其他树木配置造景和用作专题布置3种方式。     

广州市龙洞公园的设计理念和特色

基于广州市龙洞公园的悠久历史和区位优势,其设计理念强调挖掘本土文化风韵、开发文化景观、延续生态脉络,在公园设计中包括用地布局、功能结构、景观特色、道路设计、绿地种植、生态工程等方面,均展示了地域文化、生态及艺术的大地景观。     

极简主义对现代园林艺术的影响

通过对极简主义艺术的起源,特点以及理念的探讨;对极简主义园林东西方渊源的追溯及对现代园林中具有极简主义特征的实例的分析,总结出极简主义艺术在内在文化理念、外在创作形式两个方面对现代园林的影响,指出了学习西方极简主义园林对我国现代园林景观发展的实际意义。     

Progress in Research of Chinese Wetland Parks

There has been a rapid development in the construction of wetland parks in China in recent years.This paper discussed the progress in the research of Chinese wetland parks that covered the aspects of wetland resources,landscape and environment.The function of wetland parks,the evaluation of landscape and the exploiting of resources;the landscape concept and landscape planning and design;the plant environment,the water environment and the recreation environment were all reviewed.The research of Chinese wetland parks started later but developed rapidly,having remarkable achievements in practice and functional studies,and there are still some shortcomings in the researches to be improved.At last,the key points which should be researched urgently in the future were discussed.     

转录因子NAC的研究进展

NAC转录因子是植物所特有的一类转录因子,并广泛存在于不同的植物中。从NAC发现至今经过十几年的时间,NAC转录因子的结构和功能得到了较深入的研究。本文介绍了NAC家族的分类,NAC结构域蛋白的特点,NAC的生物学功能及其与植物的生长发育、抗性之间的关系。