SULCMIS Ⅲ系统应用中遇到的问题及解决方法

主要讨论在图书管理系统SULCMISⅢ的使用过程中遇到的问题及解决方法,并提出一些系统目前未能解决的问题。     

寄主植物中抑制十字花科黑腐病菌Ⅲ型分泌系统小分子化合物的鉴定

 寻找抑制植物病原菌III型分泌系统的植物源活性小分子化合物,是研发生物安全农药的重要途径之一。本研究采用水煮提取法从十字花科黑腐病菌寄主植物满身红萝卜中提取分离活性小分子化合物,利用高效液相-质谱联用解析出活性物质的单体结构。然后用荧光素酶基因luxAB构建融合报告系统以及定量PCR检测活性物质对十字花科黑腐病菌III型分泌系统的抑制效果,最后采用剪叶接种和压渗接种的方法研究活性小分子物质对十字花科黑腐病菌的生防作用。研究表明,植物鞘氨醇和二氢鞘氨醇对十字花科黑腐病菌III型分泌系统基因的转录表达有一定程度的抑制作用,但是对I、II、IV型分泌系统基因的表达没有明显的抑制作用。植物鞘氨醇在XCM1上影响菌的生长,而二氢鞘氨醇不影响菌的生长。同时,还发现这两种物质能显著降低Xcc在寄主植株满身红萝卜上的病害症状以及能够使Xcc在非寄主植物辣椒上引起过敏反应的能力丧失。该研究结果为深入研究小分子化合物对十字花科黑腐病菌III型分泌系统的作用机制及后续开发植物源抑制剂提供了一定的理论依据。     

Plant species traits regulate methane production in freshwater wetland soils

Ecosystem and biogeochemical responses to anthropogenic stressors are the result of complex interactions between plants and microbes. A mechanistic understanding of how plant traits influence microbial processes is needed in order to predict the ecosystem-level effects of natural or anthropogenic change. This is particularly true in wetland ecosystems, where plants alter the availability of both electron donors (e.g., organic carbon) and electron acceptors (e.g., oxygen and ferric iron), thereby regulating the total amount of anaerobic respiration and the production of methane, a highly potent greenhouse gas. In this study, we examined how plant traits associated with plant inputs of carbon (photosynthesis and biomass) and oxygen (root porosity and ferric iron on roots) to mineral soils relate to microbial competition for organic carbon and, ultimately, methane production. Plant productivity was positively correlated with microbial respiration and negatively correlated to methane production. Root porosity was relatively constant across plant species, but belowground biomass, total biomass, and the concentration of oxidized (ferric) iron on roots varied significantly between species. As a result the size of the total root oxidized iron pool varied considerably across plant species, scaling with plant productivity. Large pools of oxidized iron were related to high CO₂:CH₄ ratios during microbial respiration, indicating that as plant productivity and biomass increased, microbes used non-methanogenic respiration pathways, most likely including the reduction of iron oxides. Taken together these results suggest that increased oxygen input from plants with greater biomass can offset any potential stimulation of methanogenic microbes from additional carbon inputs. Because the species composition of plant communities influences both electron donor and acceptor availability in wetland soils, changes in plant species as a consequence of anthropogenic disturbance have the potential to trigger profound effects on microbial processes, including changes in anaerobic decomposition rates and the proportion of mineralized carbon emitted as the greenhouse gas methane.     

奶牛生产综合信息电脑管理系统的研究

本研究采用关系型数据库管理系统C—dBASEⅢ编制,数理统计程序采用BASIC语言编制,在IBM PC/XT(系统内存640KB以上)计算机上实现。奶牛生产综合信息电脑管理系统的功能包括:数据管理、信息查询、日常管理、报表打印、饲料配方、种公牛育种值估计、数理统计分析和奶量预测等。经运行证明,该系统的功能符合我国奶牛生产实际情况,适合在我国大、中、小型奶牛场推广应用。     

基于LISS4数据的小麦氮素营养状况反演研究

利用LISS4卫星遥感数据在小麦氮素营养状况监测方面进行了初步研究。根据搭载多光谱传感器LISS4的IRS-P6的过境周期,2006年5月3日在江苏省盐城地区进行同步地面取样测试,通过分析试验点小麦LISS4影像光谱信息与小麦叶面积指数及叶片氮含量的相关关系,发现小麦叶面积指数和叶片氮含量与LISS4影像三个波段反射率及植被指数RVI、NDVI、GNDVI均密切相关,表明利用LISS4遥感影像监测小麦氮素营养状况是可行的。比较不同波段原始反射率和植被指数,在本研究中LISS4影像波段4反射率与小麦氮素营养状况相关最密切。检验结果也表明利用LISS4影像波段4反射率估测小麦氮素营养状况是可行的。研究成果为利用遥感技术大面积监测小麦氮素营养状况和实施精确栽培管理提供了理论依据。     

Evaluation of a radioimmunoassay for type III procollagen peptide in the dog

Type III procollagen peptide (P-3-P) is a serum marker for hepatic fibrosis in humans. The utility of a commercially available radioimmunoassay for P-3-P was evaluated in the dog. The specificity of the assay was assessed by polyacrylamide gel electrophoresis (PAGE) of canine serum and purified bovine P-3-P, followed by Western immunoblotting with rabbit aniti-P-3-P serum. The sensitivity was assessed by performing the radioimmunoassay on dilutions of sera from 22 dogs. Polyacrylamide gel electrophoresis of purified bovine P-3-P and sera from two dogs suspected of having elevated P-3-P concentrations revealed no homologous bands of staining. Western immunoblotting showed marked cross-reactivity of the high antisera concentrations with several components of the serum proteins, but none corresponding to the purified P-3-P. All tested sera from dogs had minimal competitive binding with radiolabeled P-3-P in the radioimmunoassay. Dilution curves of dog sera did not parallel either the standard curve or the dilution curve of a known test human serum. There were no statistically different P-3-P concentrations in any of the groups of dogs studied. It was concluded that currently available radioimmunoassay kits for the measurement of P-3-P in the human are not applicable in the dog. Seemingly, the structure or metabolism of canine P-3-P may vary significantly from that of the bovine or human, limiting the sensitivity and specificity of this assay in the dog.