Assessment of microbial biocontrol agent (BCA) viability to mechanical and thermal stress by simulating spray application conditions

Background

In order to improve the biological control agent (BCA) efficacy, stress factors threatening the viability of microorganisms during spray application need to be determined. The effect of spray mixture temperature and exposure time on Trichoderma harzianum T 22 and Bacillus amyloliquefaciens QST713 viability were tested. Concurrently the combined effect of mechanical and thermal stress effect on BCA viability were tested at two initial spray mixture temperatures (14 and 25 °C) by simulating a spray application using airblast sprayers featured by different tank capacity and a spray liquid circuit (without and with hydraulic agitation system). To assess the BCA microorganism viability, spray mixture samples were collected at time intervals along trials and plated to count the colony forming units (CFU).

Results

The critical temperature threshold that inhibited BCA viability was 35 °C with 30 min of exposure. The sprayer type, the initial temperature of the spray mixture and the temperature increment during the trials significantly decreased the number of CFU recovered. When simulating a spray application, the spray mixture temperature increase rate was determined mainly by the residual amount of spray mixture in the tank. Even if the tank capacity does not substantially affect the final temperature reached by the spray mixture, the higher residual spray mixture in bigger tanks can expose the BCAs for a longer time to critical temperatures.

Conclusions

Experimental trials allowed us to identify the effect of factors affecting the viability of tested BCAs, providing information about the actual chance to guarantee the biological efficacy of BCA treatments. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

玉米转基因在分子农业领域的研究进展

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Feasibility for Building the Under-ground Sewage Treatment Plant in Chongqing City

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小水电站峰谷电量计算方法初探

为了正确评价小水电站的经济性,提高其设计质量,提出了一种较好的峰谷电量计算方法。首先,在指出常规计算方法没有要求计算日调节库容的弊病的基础上,提出了采取从调节库容中预留出足够的日调节库容的改进方法。接着,确定了一种较好的日调节库容计算方法。最后,给出了4种不同类的峰谷电量计算公式。     

农村污染缓流水体的修复研究

农村缓流水体是维系乡村生态平衡的重要组成部分, 水质污染已成为国内乡村缓流水体普遍现状。讨论了农村缓流水体的污染成因，并以水生植物大漂为水体修复主体，进行了大漂的水体修复特性的试验，讨论了在可控条件下用大漂对缓流污染水体进行修复治理的植物修复技术及大漂在水体修复中可能带来的生态风险及其防范。     

沈阳北部污水处理厂污泥土地利用可行性研究

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