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1.
【目的】检测新疆南疆7个县/市瓜类褪绿黄化病毒(Cucurbit chlorotic yellows virus, CCYV),进行系统发育分析,为新疆南疆CCYV预警和防控提供参考。【方法】于2019年,从中国新疆南疆7个县/市采集带有黄化特征的51份甜瓜叶片样品,RT-PCR进行CCYV的检测,进行特异性片段的克隆、测序和系统发育分析。【结果】中国新疆巴楚县、阿克苏市、莎车县、伽师县、疏勒县、疏附县均未检测到CCYV,而洛浦县的13份样本中,8份检出为CCYV阳性,检出率为61.53%,洛浦县CCYV特异性片段克隆测序获得了685 bp的核酸序列,与GenBank中的CCYV分离物外壳蛋白(Coat protein, CP)的一致性达到100%。所得序列与中国新疆(吐鲁番)、中国其他省、苏丹、日本、塞浦路斯、黎巴嫩的CP基因聚在I组(Group),沙特阿拉伯的分离物聚在Ⅱ组,伊朗的分离物聚在Ⅲ组。【结论】CCYV在中国新疆南疆洛浦县甜瓜产区发生,与中国新疆吐鲁番、中国其他省及周围国家的CCYV分离物亲缘关系很近,且群体遗传变异很小。 相似文献
2.
Large‐scale patterns of fish diversity and assemblage structure in the longest tropical river in Asia 下载免费PDF全文
Although the Mekong River is one of the world's 35 biodiversity hot spots, the large‐scale patterns of fish diversity and assemblage structure remain poorly addressed. This study aimed to investigate the fish distribution patterns in the Lower Mekong River (LMR) and to identify their environmental determinants. Daily fish catch data (i.e. from December 2000 to November 2001) at 38 sites distributed along the LMR were related to 15 physicochemical and 19 climatic variables. As a result, four different clusters were defined according to the similarity in assemblage composition and 80 indicator species were identified. While fish species richness was highest in the Mekong delta and lowest in the upper part of the LMR, the diversity index was highest in the middle part of the LMR and lowest in the delta. We found that fish assemblages changed along the environmental gradients and that the main drivers affecting the fish assemblage structure were the seasonal variation of temperature, precipitation, dissolved oxygen, pH and total phosphorus. Specifically, upstream assemblages were characterised by cyprinids and Pangasius catfish, well suited to low temperature, high dissolved oxygen and high pH. Fish assemblages in the delta were dominated by perch‐like fish and clupeids, more tolerant to high temperatures, and high levels of nutrients (nitrates and total phosphorus) and salinity. Overall, the patterns were consistent between seasons. Our study contributes to establishing the first holistic fish community study in the LMR. 相似文献
3.
LIJing LIRui ZHENGFen-li 《水土保持研究》2004,11(4):174-176
Erosion and transport of soil has worldwide implications for agriculture, landscape stability, climate, natural hazards, and clean, renewable resources of water and air. Assured access to clean water and a healthy and safe environment requires an ethic of conservation and protection. The minimum scale in which these principles apply successfully is basin wide. These are the fundamental concerns of the Sino-US Centers for Soil and Water Conservation and Environmental Protection. 相似文献
4.
D. Bilalis P. Efthimiadis & G. Karagiannis 《Journal of Agronomy and Crop Science》2001,187(2):121-126
The effect of three herbicides, diclofop-methyl (DM), imazamethabenz-methyl (IM) and fenoxaprop-ethyl (FE), was investigated at the recommended rate (×) and double the recommended rate (2×) in a durum wheat crop. FE had the smallest effect on root system characteristics, while IM had the greatest effect. For all herbicides, the double rate treatment gave a greater statistical reduction than the single rate for root system characteristics. FE had the smallest effect on the activity of aerobic microorganisms, while DM gave the greatest reduction, 10 days after application, and all three herbicides reduced earthworm abundance. DM gave the smallest reduction in the number of tillers and in plant height, while the double rate of IM gave the greatest reduction. For all three herbicides, double the recommended rate resulted in a significant reduction in the weight of 1000 seeds, while no statistically significant deviations were noted at the recommended rate. Finally, the use of all three herbicides resulted in significantly lower statistical yields compared to the sample (control plot), although there were no significant statistical differences among them, for either recommended or double recommended rates. 相似文献
5.
介绍了开发区大气、水环境容量计算及管理方法,并以湖南城陵矶经济技术开发区为例,初步了方法的具体应用和有关细节处理,所介绍的方法将对其他类似开发区大气、水环境容量的计算及管理起到一定的借鉴和指导作用。 相似文献
6.
DONOSO Leonito Acosta 《中国林业科技(英文版)》2005,(1)
The Luntiang Kamaynilaan Project (Metro ManilaGreening Project) launched in 1988 planted trees inmajor thoroughfares, center islands, school premisesand other idle public lands, was basically a governmenteffort. When the funds ran out, the project also fizzledout. In both projects however, it is evident that the keyfactor is peoples participation.Gaining lessons from the past two projects, thegovernment established a collaborative effort ofgovernment and non-government organizations. TheCl… 相似文献
7.
畜牧业生产中的环境污染及治理对策 总被引:22,自引:3,他引:19
本文阐述了畜牧业生产中产生的畜禽粪尿、有机废水等废弃物对环境造成的污染,探讨了治理畜牧业污染的措施,提出了发展生态畜牧业的思路。 相似文献
8.
从不同角度评述了森林在哈尼族传统人居环境体系中的作用和地位,阐述了未来山区村镇发展中保留周边森林的重要性。 相似文献
9.
10.
用电沉积法制备Mo-W-Ni-Pb-Ca多元活性阴极,研究了在0.5mol/L H2SO4中的析氢反应,结果表明,多元阴极具有较高的电催化活性,其析氢过电位比软钢阴极降低305mV,i0提高三个数量级,且有较好的稳定性,并用光电子能谱研究了表面组成和结构。证实表面有:Ni,NiO,MoO2,MoO^2-4,WO3,PbO2,PbO,Ca^++等物质。 相似文献