The Effect of Tryptophan on Serotonin-Like Neurons in Duck Cerebellum

Healthy Cherry Valley ducks were used in the present study. Different doses of tryptophan were injected intraperitoneally to them after being fasted 4 h （8：00 a.m.-12：00 a.m.）. One hour later, they were deeply anaesthetized and perfused. The cerebellum was removed to make serial paraffin longitudinal sections. The streptavidin-biotin-peroxidase complex （SABC） method was used to study the distribution of serotonin-like neurons in the cerebellum. All films were analysed by using a computer-assisted image analysis system. Serotonin-like neurons are only localized in cerebellar Purkinje cell layer. The optical density averages of serotonin-like neurons in 200 and 100 mg kg^-1 group are significantly higher than that of 0 mg kg^-1 group （P〈0.01）. These results show that serotonin-like neurons are distributed in Purkinje cell layer and that excessive tryptophan can affect the content of serotonin in cerebellum.     

复合菌剂发酵秸秆的作用及应用

以玉米秸秆为试验材料,研究了复合剂的添加量及发酵条件对对玉米秸秆纤维素和粗蛋白含量的影响.结果表明:在温度为25℃,菌种和秸秆比例为1:300的条件下,有氧发酵60、120、180、240、300和360 h后,秸秆纤维素含量分别下降了1.25%、5.09%、8.08%、8.51%、8.85%和9.31%,粗蛋白含量提...     

大枣多糖铁的合成及其铁含量测定

[目的]从大枣中提取大枣多糖,然后与铁（Ⅲ）合成大枣多糖铁配合物（JPC）,建立JPC中铁含量的测定方法。[方法]采用热水浸泡法提取大枣多糖;在碱性条件下,大枣多糖水溶液与三氯化铁反应合成JPC;采用邻菲罗啉分光光度法和原子吸收光谱法测定JPC中铁（Ⅲ）的含量。[结果]JPC是深棕红色无定型粉末,JPC中铁（Ⅲ）的含量：邻菲罗啉分光光度法测定结果为19.76%,原子吸收光谱法测定结果为20.37%。[结论]邻菲罗啉分光光度法和原子吸收光谱法的测定结果基本一致。     

不同添加剂对蛋鸡生产性能和蛋品质的影响

为探讨不同添加剂对蛋鸡生产性能的影响,选用1 500只280日龄海兰褐蛋鸡,随机分为5组,每组5个重复,每个重复60只;对照组饲喂基础日粮,基础日粮中分别添加0015%土霉素、001%微生态制剂、01%中草药提取物和5%新型生物复合预混剂为试验Ⅰ\|Ⅳ组,研究几种添加剂对海兰褐蛋鸡生产性能和蛋品质指标的影响,试验期30 d。结果表明：基础日粮中添加微生态制剂、中草药提取物和新型生物复合预混剂,其蛋鸡产蛋性能和蛋白质均优于对照组和添加土霉素组,且以添加5%新型生物复合预混剂组蛋鸡生产性能更佳。     

有机无机复合体防治黑土退化的可行性研究

就黑土退化现状、有机无机复合体的研究现状、有机无机复合体与土壤退化的关系等方面的研究成果进行了综合评述,指出有机无机复合体是土壤改良剂、营养源和重金属或有机有毒污染物过滤器,在土壤退化防治研究中,应加强土壤有机无机复合体的研究。     

红壤复合胶体腐殖质的特性

对闽北6个样品测定结果表明,红壤复合胶体中C、N含量较高,其腐殖质的C/N和HA/FA、胡敏酸的芳构化度和腐殖质氧化稳定性系数均较重组小,而松结合态/紧结合态腐殖质的比值则较大.这些特性有利于土壤养分保存与供应和改善土壤结构.     

Interaction of benzimidazole-N-sulfonamides with the cytochrome b/c1 complex of Pythium aphanidermatum

Experiments with intact cells and submitochondrial fractions of Pythium aphanidermatum (Edson) Fitz. indicated an interference of benzimidazole-N-sulfonamides with the NADH- or succinate-driven electron transport system between cytochromes b and c. Comparison with Ustilago maydis (DC) Corda and Botrytis cinerea Pers. ex Fr. revealed that this effect is Oomycetes specific. The molecular interaction between benzimidazole-N-sulfonamides and the mitochondrial cytochrome b/c₁ complex from P. aphanidermatum has been investigated. Binding assays with [¹⁴C]52232 RP (dimefluazole) indicated a time- and dose-dependent labelling of two proteins. The molecular mass of one labelled protein and the competition of the binding with antimycin A suggest that benzimidazole-N-sulfonamides interact with the Q₁-centre of cytochrome b. Furthermore, experiments with doubly labelled [³H][¹⁴C]CGA 323103 revealed a possible irreversible inactivation of the b/c₁ complex leading to covalent linkage of the dimethylsulfonamoyl moiety to the target site.     

日本血吸虫候选疫苗免疫保护力研究进展

血吸虫病是危害严重的寄生虫病之一,其疫苗的研究是血吸虫病防治的一项工作重点也是一大难题。本文综述了日本血吸虫疫苗侯选分子筛选、模拟表位疫苗和多价疫苗的研究现状,并对血吸虫疫苗的深入研究进行了分析。     

Study on the New Technology of Extracting PD in Strong Noise Environment With Complex Wavelet

Based on existing research, this paper analyzes and compares the phase information characters between partial discharge signal (PD) and its disturbance, and also extracts the feature of Partial Discharge with complex wavelets in noise environment. The research demonstrates that complex wavelets are more superior to original real wavelets in identifying Partial Discharge signal, and those simulated applications and the datum from laboratory can guarantee the effectiveness of the extraction of the feature of Partial Discharge and the restraint of the strong electromagnetic disturbance.     

我国玉米穗腐病致病镰孢种群及禾谷镰孢复合种的鉴定

为阐明中国玉米镰孢穗腐病的主要致病镰孢菌种类及其分布特征,采用形态学、培养特征及特异性分子鉴定方法,对采集自我国18省100个县的玉米籽粒样品进行分离鉴定,并通过TEF-1α基因序列测定解析禾谷镰孢复合种的构成.结果表明,在我国引起玉米穗腐病的主要致病菌为镰孢菌,分离频率为56.0%,其次还有青霉菌、曲霉菌、木霉菌等.138个镰孢菌分离物中鉴别出7个种及复合种,其中拟轮枝镰孢菌Fusarium verticillioides(56.5%)和禾谷镰孢复合种F.graminearum species complex(37.7%)为广泛分布的优势致病种类,其余为黄色镰孢菌F.culmorum(2.2%)、层出镰孢菌F.proliferatum(1.5%)、尖镰孢复合种F.oxysporum species complex(0.7%)、茄镰孢复合种F.solani species complex(0.7%)和亚粘团镰孢菌F.subglutinans(0.7%).在禾谷镰孢复合种中鉴定出3个独立种:广泛分布的禾谷镰孢菌F.graminearum sensu stricto(59.6%)、分布在云南、贵州及陕西商洛等南方生态区的南方镰孢菌F.meridionale(25.0%)和分布在内蒙古、吉林、山西、河北及北京等北方生态区的布氏镰孢菌F.boothii(11.5%).