A reactive pattern of ischemia-induced nestin protein in the rat brain

AIM:To explore the expressive profile of nestin protein in the focal ischemic brain and to study the recovery mechanism of brain focal infarct.METHODS:Cellular morphology,time-course and distribution pattern of nestin positive response were immunohistochemically examined in different brain regions of 36 adult male SD rats. RESULTS:Nestin positive response of different brain regions in sham operated rats was present in small- and micro-vasculartures and the third ventricle bottom and ependyma. A large number of nestin positive cells were detected in ischemic brain, and were more remarkable in the cortical areas of parietal lobe and preoptic area as well as ischemic caudoputamen. Stellate nestin positive cells were located in the deep layer of ischemic cortex, but fibrillary cells were located in the shallow layer. Nestin positive cells in the ischemic caudoputamen showed the same changes of morphology as those cells in the deep layer of ischemic cortex. Morphological and number alterations of nestin positive cells were the most remarkable at 1 weeks post-ischemia, which showed more hypertrophy and proliferation in morphology, and a marked increase in number was present in the ischemic cerebral cortex and the ischemic caudoputamen. These alterations of nestin positive cells persisted up to 6 weeks post-ischemia, and then, the nestin positive response in the ischemic brain decreased gradually.CONCLUSION:Focal cerebral ischemia induces nestin re-expression on reactive astrocytes, which may be very important to the self-recovery of cerebral infarct.     

Changes of myocardial glycogen content and right ventricular function in hypoxia swimming rats

AIM: To investigate the changes of myocardium glycogen content and the relation ship between changes of the myocardial glycogen content and the myocardial function. METHODS: Male Wistar rats were placed in (hypoxia rats) and made to swim in (hypoxia swimming rats) a hypobaxic chamber simulating an altitude of 5 000 m above sea level. The content of myocardium glycogens was determined by colorimetry. RESULTS: The myocardium
glycogen content of rats significantly reduced along with the prolongation of hypoxic exposure and approached to control level in hypoxia swimming rats. The myocardial function of right ventricule was improved significantly compared with control group.CONCLUSION: Moderate exercise (swimming) is beneficial to hypoxic adaptation of rats under the condition of chronic hypoxia.     

Olive processing wastes for weed control

The herbicidal effect of olive processing wastes (OPW) on some weed species in wheat, maize and sunflower was investigated in the Aegean region of Turkey. In trials with maize and sunflower, OPW was applied as an air‐dried solid form at 3 and 4.5 kg m^?2. It provided an effectiveness level on Portulaca oleracea of 63–98%. In trials with wheat, OPW was applied as solid and liquid forms, each at two different doses, namely 4.5 and 6 kg m^?2 (solid), and 5 and 10 L m^?2 (liquid). Solid OPW provided a reduction in total weed coverage of 75% and 81% at doses of 4.5 and 6 kg m^?2, respectively. The weed coverage reduction by liquid OPW was 39% and 62% with 5 and 10 L m^?2, respectively. Apart from 12–26% reduction of the number of germinating seeds, OPW showed no toxic effects on maize and sunflower. Wheat was affected in the initial stages but no adverse effect was detected at harvest. It can be concluded that the herbicidal effect of OPW may be considered as an alternative to chemical weed control in some important summer crops (maize and sunflower) and for most of the weeds in winter wheat.     

新疆柽柳幽柽木虱的生物学特性

在新疆的柽柳属植物上发现3种木虱，对其中的柽柳幽柽木虱进行了研究。该种木虱在新疆阜康地区1年发生1代，以4龄若虫在柽柳多年生枝条上越冬。越冬若虫翌年4月中旬开始取食，并使枝条顶端形成虫瘿，若虫在虫瘿内发育到5龄，至6月开始羽化，成虫平均寿命7．3d。雌成虫将卵产在花苞内，若虫在花苞内取食形成虫瘿，4龄若虫10月中旬爬出虫瘿转移到多年生枝条上越冬。发现若虫寄生蜂1种。     

仔猪日粮中添加复合酸化剂的饲喂效果

选择7日龄哺乳仔猪126头，在玉米—豆粕型日粮中，分别添加6种自行研制的复合酸化剂饲喂仔猪，酸化剂的主要成分是柠檬酸、延胡索酸、磷酸、乳酸、草酸、酒石酸。结果表明，断奶仔猪的日增重比对照组分别提高11．94％、14．03％、7．46％、7．46％、17．31％、12．24％；饲料转化率分别提高13．92％、25．26％、7．73％、11．35％、21．65％、11．86％。仔猪的腹泻率也降低了。     

遗传与环境在生物进化中的关系

本文讨论了在生物进化的过程中遗传与环境的关系及遗传物质的范围和生物可塑性的问题。遗传物质的变异是进化的内因，环境对遗传物质的变异起到诱发与筛选的作用，进化后的生物对环境又有反作用。     

纳米微粒材料生物学活性研究进展

综述了纳米微粒的生物学效应及其免疫调节、抗菌、抗肿瘤等多种生物学活性.     

富铁酵母对家兔红细胞免疫功能的影响

为探讨富铁酵母对家兔红细胞免疫功能的影响,将32只家免随机分成富铁酵母高、低剂量组,FeSO4组及空白对照组,分别灌服富铁酵母(Fe元素8 mg/kg、4 mg/kg)、FeSO4(Fe元素4 mg/kg)及空白生理盐水;连续5 d后,检测红细胞C3g受体花环率和复合物(IC)花环率.结果:富铁酵母较FeSO4及空白组能显著提高红细胞C3h受体花环和红细胞复合物(IC)花环的百分率,但高、低剂量间无显著性差异.结论:富铁酵母能显著提高红细胞免疫功能.     

金龟子绿僵菌固态培养生物变量优化研究

金龟子绿僵菌属于真菌类生物杀虫剂,本文研究了接种量、种龄、菌种代数等生物因素对金龟子绿缰菌生长及产孢量的影响,并对液—固两步发酵工艺进行了研究。经过优化,得到金龟子绿僵菌产孢的最佳生物参数是:接种量为2.5g/100g、种龄为5-6天;采用固体二代种,培养6天,孢子产量可达1.531×10~(10)孢子/g干培养基。     

薇甘菊天敌安婀珍蝶生物学特性的研究

安婀珍蝶Actinote anteas(Doubleday ＆ Hewitson)是薇甘菊的天敌,取食薇甘菊Mikania micrantha Kunth.的叶片,能有效的控制薇甘菊的蔓延和生长。在广州市和深圳龙岗进行的观察表明,在实验室条件下,安婀珍蝶一年生长3代~4代。世代平均历期为112.44±1.18天;卵期平均为11.44±1.18天;幼虫期平均为82.54±4.05天;蛹期平均为12.98±1.31天;成虫期平均为7.33±0.80天。本文还记述了该蝶各虫态的形态特征、生活习性及其天敌等。