Identification of a novel miRNA from the ovine ovary by a combinatorial approach of bioinformatics and experiments

MicroRNAs (miRNAs) are a class of short endogenous, single-stranded, non-coding small RNA molecules, about 19–25 nucleotides in length that regulate gene expression at the translation level and influence many physiological process, such apoptosis, metabolism, signal transduction, and occurrence and development of diseases. In this study, we constructed a library from the ovine luteal phase ovary by using next-generation sequencing technology (Solexa high-throughput sequencing technique) and identified 267 novel miRNAs by bioinformatics. One of the novel miRNAs (ovis_aries_ovary-m0033_3p), which expressed in the sheep ovary and testis, was confirmed by real time PCR and northern blot. Ovis_aries_ovary-m0033_3p was 21 nucleotides in length and located on chromosome 12, and it had 100% similarity to hsa-miR-214-3p, mmu-miR-214-3p, dre-miR-214and ssc-miR-214. Meanwhile, the pre-miRNA was 82 nucleotides in length and had a standard hairpin stem-loop structure. From the consistency of the sequence and structure, we speculated that ovis_aries_ovary-m0033_3p had a function similar to hsa-miR-214-3p, which is involved in the fine regulation of cell survival, embryonic development, breeding activities and resistance to ovarian cancer, so we defined it as oar-miR-214-3p. These experimental results will enrich the miRNA database for ovis aries and provide the basis for researching the regulation mechanism of miRNA in relation to breeding activities of seasonal breeding animals.     

甘肃干旱荒漠区纸浆林三倍体毛白杨营建试验

本文分析了2001年作为纸浆林发展引种的10个三倍体毛白杨系号在石羊河流域造林的生长适应性。结果表明:在同一立地类型和同等抚育条件下,A129、A128、B330、A125、B1008、BT85、A122等7个无性系杂种三倍体毛白杨幼林在石羊河流域速生、抗寒、耐盐碱、抗黄斑星天牛,在石羊河流域表现出较强的适应性和抗逆性,生长量显著高于普通毛白杨、群众杨、新疆杨;由于其速生性使黄斑星天牛不能完成生活史而不至于造成危害;在专用纸浆林方面大力推广具有良好的发展前景。     

20%苍耳素乳油对梨黑星病的田间药效试验

采用20%苍耳素乳油对梨黑星病进行田间药效试验,结果表明:20%苍耳素乳油100倍液第1次施药后7 d和末次施药后14 d对叶片的防效为72.05%和66.41%,对果实的防效为52.49%和61.56%,优于50%多菌灵800倍液。     

兰州市安宁区蔬菜地土壤酸度及重金属的测定和评价

以甘肃省兰州市安宁区的5块菜地土壤为研究对象,采用电位法测定了土壤pH及土壤中Cu、Pb、As、Zn、Cr和Ni的含量,并根据GB 15618-1995土壤环境质量二级标准通过单项质量指数与综合质量指数相结合的方法对土壤重金属的环境质量状况进行了评价.结果表明:兰州市安宁区蔬菜土壤环境质量等级为1～2级,属于尚清洁水平,符合无公害蔬菜生产的土壤环境质量要求.但土样之间重金属含量存在较大的变异性,反映了人为活动已对土壤重金属含量产生了明显的影响,需加强监测和预防.     

2种三点金草对铝盐毒害忍耐的初步研究

研究三点金Desmodium triflorum的耐铝能力对开发优秀的草坪地被植物有一定的重要性,研究表明,2种三点金草对Al3 有一定的耐受性,低铝浓度(20 mg/kg)对三点金草幼苗和成苗生长都有一定促进作用,但较高铝浓度(40 mg/kg)均会阻碍两者根系生长,使其侧根、根干质量和根冠比都有明显减少.在幼苗期,大叶三点金根系对Al3 有较高程度的耐性,但当两者再生长30 d后,小叶三点金对铝盐的耐受能力增强,相反大叶三点金则有所下降.     

张掖市规模化羊场疫病综合防制技术研究

随着张掖市养羊业规模化、集约化程度的不断提高,羊的疫病也随之频发,呈现多系统混合感染、隐性感染、引入性疫病致病率高等特点,本文论述了规模化养羊场疫病综合防制的基本原则,提出了对规模化养羊场疫病综合防制具有指导意义的技术措施.     

特色农产品物流数字化平台的构建与研究

分析了建设特色农产品物流数字化平台的必要性,提出了构建系统平台的具体方案,并对其前景和经济效益做出了分析。     

Visual Basic语言循环结构的教学研究

本文介绍了Visual Basic语言的4种循环结构,提出了正确理解和设计不同循环结构的方法与步骤,对常用的循环结构通过实例进行了验证。     

不同添加剂对低水分紫花苜蓿青贮品质的影响

研究了不同添加剂(麸皮、红糖、甲酸、乙酸、乳酸菌制剂等)对含水量控制在40%~45%的全株紫花苜蓿(Medicago sativa)青贮饲料质量的影响。青贮90d后,对开封后的青贮饲料进行了感官评定及实验室测定,探讨了低水分紫花苜蓿青贮过程中发酵品质的变化及添加剂对其影响。结果表明:经添加剂处理的低水分紫花苜蓿,发酵品质均有不同程度的提升,其pH及氨态氮含量降低。芯来旺I号,max200和红糖对低水分紫花苜蓿青贮饲料的发酵品质和营养价值有明显改善,在90d的短期青贮中,是适宜进行低水分紫花苜蓿青贮饲料生产的高效添加剂。芯来旺I号作为青贮添加剂在此次试验中效果最佳,甲酸最差,而麸皮作为青贮添加剂的适宜剂量有待进一步讨论。     

铺网和不同栽培基质对草皮质量的影响

通过田间试验,研究了铺网和不同栽培基质组合对草皮质量的影响,以期为网草皮生产提供依据。结果表明,铺网能显著提高草皮强度,降低草皮破损率,缩短成卷时间。农田土(60%)+羊粪(20%)+河沙(20%)成坪和成卷时间最短,草皮生长速度最快,质地最优,颜色最深,密度和强度最大;农田土(100%)草皮破损率最小;河沙(60%)+农田土(40%)草皮重量最轻。草皮综合质量评价显示,C基质即农田土(60%)+羊粪(20%)+河沙(20%)C+尼龙网是生产草皮的最佳组合。