硼对草坪草超氧化物歧化酶活性、超氧阴离子产生速率和丙二醛含量的影响

采用基质培养法,设缺硼和施硼2种处理,在早秋和春季测定了草坪草超氧化物歧化酶(SOD)活性、超氧阴离子(O2)产生速率和丙二醛(MDA)含量,研究硼对草坪草活性氧代谢的影响,以探讨提高草坪草抗寒性的措施.结果表明:和早秋相比,2种草坪草海滨雀稗和矮脚虎春季SOD活性明显下降,超氧阴离子产生速率和丙二醛含量均明显升高,表明受到了春季低温伤害;施硼显著提高SOD活性,显著降低超氧阴离子产生速率和丙二醛含量,在春季尤为明显;表明施硼提高草坪草保护酶SOD活性,降低了活性氧及膜脂质过氧化对生物膜的伤害,提高了草坪草抗寒性.而2种草坪草相比,海滨雀稗抗寒性高于矮脚虎.     

Islet cells modified with PD-L1-GPI molecule inhibit attack of reactive T cells

AIM: To investigate the immune depressive effect on the reactive T cells and to explore the immunologic injury mechanism of beta cells of islet in type 1 diabetes mellitus (DM-1). METHODS: pAd5/ PD-L1-GPI adenovirus vector with target gene was constructed and transfected into NIT cells which are known as a mouse insuloma cell line. The highly expressed membrane protein of PD-L1-GPI was confirmed by Western blotting. The peripheral blood non-adherence lymph leukocytes and target cells were cultured to detect lymph leukocyte proliferation and the T cell function. The level of IL-2, TNF-α and IFN-γ were detected in the cell culture fluid. RESULTS: Compared with the control group, the NIT cells modified with PD-L1-GPI inhibited the sensitized lymph leukocyte proliferation effectively and down-regulated the level of some cytokine secretions such as IL-2, IFN-γ and TNF-α (P<0.05). CONCLUSION: The islet cells expressed the PD-L1 gene inhibit the reactive responsive T cells, block up the cytotoxic effect of autoimmunity T cells, and induce the immunotolerance, which would be a value direction of the therapy of DM.     

硼对豌豆根尖铝毒的影响

研究了B(0、 100、 1000μmol/L)对豌豆根尖Al和胼胝质含量的影响,以揭示B是否能减轻Al胁迫.发现:Al浓度100 μmol/L时,浓度100 μmol/L B处理的根苏木精染色颜色较不加B处理浅.Al浓度100 μmol/L和500μmol/L时,100μmol/L B处理的豌豆0～0.5 cm和0.5～1 cm根段的Al含量均比未加B处理有明显降低的趋势;高B处理(1000 μmol/L)均引起根段的Al含量高于不加B处理.Al浓度100 μmol/L时,加B处理后豌豆0～0.5 cm根段胼胝质含量显著低于不加B处理.结果表明:适量B可以减轻豌豆根尖Al毒.     

食品安全校内实习基地建设与管理探索与实践

食品安全校内实习基地是学生进行食品安全实习活动的重要场所。该文介绍食品安全校内实习基地建设的重点,提出实习基地管理的措施。     

高效液相色谱-荧光检测法测定水产品中孔雀石绿残留量

将水产品样品中残留的孔雀石绿用硼氢化钾还原为其代谢产物隐色孔雀石绿,经前处理试剂盒提取,固相萃取柱净化、反向色谱柱分离,用液相色谱仪荧光检测器检测。方法的回收率在84.5%～92.1%,相对标准偏差4.09%～5.26%,检出限为0.5滋g/kg。结果表明:该方法检测孔雀石绿前处理过程简单,灵敏度高,能快速对大量样品进行定量分析。     

布氏杆菌病诊断技术研究进展

布氏杆菌病(brucellosis)是由布氏杆菌(Brucella)感染引起的以流产、生殖器官炎症、关节炎为主要症状的慢性人兽共患病,主要危害人和动物的生殖健康,侵害多个组织器官,不易治愈,我国将其列为二类动物传染病。近年来,我国各省份报告病例呈上升趋势,防控形势日益严峻,而及时准确地诊断对预防和控制布氏杆菌病具有极其重要的作用。本文从布氏杆菌病的流行病学、临床特征、细菌学诊断、血清学诊断、分子生物学诊断、分子分型技术等方面的研究进展进行综述,以期为该病的诊断提供参考。     

城市快速路环境保护验收调查结果研究分析

文章以佛山市桂丹路一期改造工程(含谢叠大桥)环境保护验收调查为例,根据项目特点,确定以敏感点变化、声环境现状、环境监理与管理、公众参与调查等为竣工环境保护验收重点,并将调查结果与环评结论进行对比,探讨城市快速路环境保护验收存在的问题,并提出相应的建议和解决方法。     

4种花灌木乔木化育苗技术研究

为了摸索如何把花灌木育成树状小乔木的育苗技术,选择了园林常见多花的扶桑、红花夹竹桃、黄花夹竹桃、紫薇等4个树种,采用保留单一中轴主枝,并按主枝全苗、主枝中截和主枝截顶三个处理方法,通过头年密植育干,第二年疏植养干,第三年储冠的育苗技术,育成了树状小乔木,改进了育苗期整形修剪技术.进一步提高了园林观赏效果.     

Effect of cold storage on vitamin C,phenolics and antioxidant activity of five orange genotypes [Citrus sinensis (L.) Osbeck]

The effect of cold storage on antioxidant profile and the antioxidant activity of five sweet orange genotypes [Citrus sinensis (L.) Osbeck], three blood (pigmented) varieties with different anthocyanin contents (‘Tarocco Messina’, ‘Tarocco Meli’ and ‘Moro’) and two blond varieties (‘Ovale’ and ‘Valencia late’), stored at 6 ± 1 °C for 65 d was investigated. During fruit storage, anthocyanins, hydroxycinnamic acids, vitamin C, flavanones and total phenolics were determined, and juice antioxidant capacity was measured by two different in vitro tests (DPPH scavenging activity and inhibition of induced linoleic acid peroxidation). The results showed an increase in anthocyanins, flavanones and hydroxycinnamic acids and a slight decrease in vitamin C in the blood oranges. Cold storage negatively affected flavanone concentration, while positively influenced vitamin C in blond orange varieties. Both antioxidant activity tests showed an increase in antioxidant capacity during storage caused mainly by phenolic accumulation (blood oranges) and vitamin C increase (blond oranges). Finally, correlations between antioxidant activity and total or individual phenolic components were examined.     

试论做好图书馆卫生防疫工作

 

探讨了图书馆做好卫生防疫工作的重要性，并从图书馆建馆选址、图书馆室内环境卫生、图书等公用物品消毒、图书馆工作人员的卫生防护等四个方面入手对如何做好图书馆卫生防疫工作展开了论述。