Luteolin prevents fMLP-induced neutrophils adhesionvia suppression of LFA-1 and phosphodiesterase 4 activity

Luteolin is an active ingredient found early from Folium perillae and Flos lonicerae, and has a specific inhibition on phosphodiesterase 4(PDE4) activity in vitro. Researches show luteolin has pharmacological effects of anti-inflammation, anti-anaphylaxis, antitumor, antioxidant, protection of nervous system and so on, and has mainly been used for the treatment of respiratory inflammatory diseases, cancer and cardiovascular disease in clinic. PDE4, specific to hydrolyze cyclic AMP(c AMP), is considered to be a new anti-inflammatory target due to the decisive role on c AMP signal in inflammatory cells such as neutrophils. In order to explore the anti-inflammatory mechanism, we further studied the effects of luteolin on the activity and expression of PDE4, the expression of lymphocyte function-associated antigen-1(LFA-1) and macrophage-1(MAC-1) in neutrophils, and the adhesion of neutrophils and endothelial cells. The results showed that luteolin had a dose-dependent inhibition on both bare PDE4 activity and PDE4 in cultured neutrophils, and had an obviously promotive effect on gene expressions of PDE4 A, 4B and 4D in later period. Luteolin had a significant inhibitory effect on neutrophils adhesion and LFA-1 expression in early stage, and had no obvious effect on MAC-1 expression. Therefore, luteolin can inhibit LFA-1 expression of neutrophils, then inhibit the adhesion of neutrophils and endothelial cells, and the mechanism is at least related with the inhibition of PDE4 activity.     

Extracellular and Intracellular Calcium both Involved in the Jasmonic Acid Induced Calcium Mobilization in Arabidopsis thaliana

The objective of this experiment is to evaluate the role of intracellular and extracellular Ca2＋ and calmodulin （CAM） in jasmonic acid （JA） signaling. The laser scanning microscopy was used to detect the changes of [Ca2＋]cyt of Arabidopsis thaliana leaf cells which pretreated with different types of calcium channel blocker. Moreover, the expression of VSP, one of JA response genes, was also investigated after pretreated with the above blocker and antagonist of CaM. The results showed that extracellular and intracellular calcium both involved in the JA-induced Ca2＋ mobilization, and then Ca2＋ exerted its functions through activating the CaM or CaM related proteins. The apoplast calcium influx and the calcium release from the calcium stores are both involved in the JA-induced calcium mobilization, then the JA-induced Ca2＋ transmited the JA signal through CaM or CaM related proteins, and regulated the JA responsive genes.     

猪传染性胃肠炎病毒S基因A抗原位点的克隆及原核表达载体的构建

 【研究目的】对猪传染性胃肠炎病毒S基因A抗原位点进行克隆和原核表达载体的构建。【方法】参考GenBank上公布的TGEV S基因A抗原位点序列,应用Primer6.0设计一对含酶切位点的引物,用于RT-PCR扩增A抗原位点目的片段,将扩增产物连接于paesy-T克隆载体上构建克隆载体,用EcoR I和Xhol I对表达载体PET-32a（+）和重组质粒进行酶切,将酶切产物亚克隆至PET-32a（+）多克隆位点上,连接、转化至BL21(DE3),构建A位点的原核表达载体,并对阳性重组质粒进行酶切、PCR和测序鉴定。【结果】所扩增的目的片段的大小为534bp,与原核表达载体连接后,经核苷酸及推导的氨基酸序列分析表明,该基因与其它猪传染性胃肠炎病毒相应基因具有很高的同源性,说明成功地构建了TGEV S 基因A抗原位点的原核表达载体。【结论】TGEV S 基因A抗原位点原核表达载体的成功构建,填补了中国国内单独针对此位点进行研究的空白,也为TGEV诊断方法的建立提供良好的技术基础。     

不同基质处理对天竺葵扦插苗生长的影响

 采用不同基质进行天竺葵扦插试验,对天竺葵扦插苗生长情况调查结果表明：珍珠岩1:蛭石1混合基质提高了天竺葵扦插苗的成活率,促进其根、茎和叶鲜重和干重的增加,同时还促进了扦插苗最长根的伸长生长。综合来看,珍珠岩1:蛭石1混合基质更有利于天竺葵扦插苗的生长。     

中国兰属植物菌根真菌的AFLP多样性分析

对19株分离自不同地理分布和生态型的中国兰属植物菌根共生丝核菌进行了AFLP分析,12对引物组合共扩增出1 869条带,其中1 016条具有多态性,平均多态性比率达54.4%。AFLP分析结果采用UPGMA聚类,19株菌的相似系数在0.61～0.89之间;聚类关系显示兰花地理分布、所属种和生态类型是影响中国兰属植物菌根真菌多样性及其与兰花间专一性关系的关键性因素。地生型的春兰和云南的附生兰与其共生菌根真菌之间有着较严格的专一性关系。     

干枣制作蜜枣过程中色泽变化的研究

通过研究复水、烘烤条件对加工工艺过程对干红枣色泽变化的影响,结果表明：由于加工温度和加工时间促进枣中非酶褐变及色素物质的变化,因此加工温度、时间对色泽变化的影响很大。即加工中降低温度、缩短加工时间可提高蜜枣的感官质量。     

稀土添加剂对猪粪堆肥的影响

首次将稀土添加剂应用于有机物发酵,各种添加剂对促进猪粪堆肥腐熟和提高堆肥品质均有较好的效果,其中稀土国光的综合性能最佳。添加剂对堆肥的促进作用可归因于其能有效促进猪粪堆肥中微生物的生长、繁殖,从而增强了微生物对有机物的分解作用,使堆肥快速成熟。     

大豆实验室PEG6000模拟干旱体系的建立

本实验以北京广泛种植的三个大豆品种：早熟18、中作95-888M3、中作95-888M3绿粒变异为材料,比较研究了在5%、10%、15%、20%、25% PEG6000渗透胁迫下大豆植株体内NR、CAT、POD活性,以及chl、游离pro含量的变化。结果表明,在5%PEG6000渗透胁迫处理下,各生理指标出现了显著性差异。实验室模拟干旱处理的最佳PEG6000浓度为15%。     

北京地区中苜一号苜蓿年内草产量及品质分析

2002年在北京农学院农学试验站内对紫花苜蓿中苜一号各茬的株高、干草产量、粗蛋白含量的变化进行了分析。结果表明中苜一号紫花苜蓿全年干草总产量9582.3kg/hm2,第二茬与第一茬和第三茬的干草产量和株高存在显著差异。中苜一号苜蓿年内各茬粗蛋白含量依次是19.82%、11.70%和21.14%,第二茬与第一、三茬的粗蛋白含量差异达显著水平,除第二茬苜蓿等级评价为中等外,其余两茬都为上等。苜蓿加工调制的方法不同,也会影响其营养物质的含量。     

作物区试中品种稳定性评价的秩次分析模型

本文提出了一种用于作物区域试验中度量非参数资料品种稳定性的模型。 介绍了 品种稳定性评价的统计数估算、 统计检验及品种稳定性分类的具体方法。