Variation of serum NSE and S-100 β in patients with acute cerebral infarction subtypes

AIM: To explore clinical significance of the serum changes of neuron-specific enolase (NSE)and S-100 β protein (S-100 β) during acute cerebral infarction. METHODS: 59 acute cerebral infarction patients were classified as total anterior circulation infarcts (TACI), partial anterior circulation infarcts (PACI), lacunar infarcts (LACI) and posterior circulation infarcts (POCI). Their serum NSE and S-100 β concentrations were determinated by enzyme linked immunosorbent assay (ELISA) during stroke onset 6 d, and compared with 32 controls. RESULTS: The every time point serum NSE concentration of TACI was higher than controls (P＜0.01), and its highest value occured at 3 d after the onset. The every time point concentration of PACI was also higher than controls (P＜0.05), its highest value occured at 1 d after the onset. The increment of serum S-100 β synchronized with serum NSE change in TACI. The S-100 β of PACI started to increase at 3 h after the onset, its highest value occured at 1 d after the onset, and concentration at 6 h, 1 d, 3 d and 6 d was markedly higher than controls (P＜0.05). However, the every time point NSE and S-100 β concentrations of LACI and POCI increased unmarkedly compared with control group. CONCLUSIONS: The serum NSE and S-100 β changes in acute period (contains acute early period) of cerebral infarction subtypes might be different. These results might help to treat acute cerebral infarction according to the classification.     

ECM-ISC框架下期望确认度对在线知识问答社区持续使用意愿的影响研究

[目的/意义]研究影响用户持续使用在线知识问答社区意向的因素,深入理解用户接受并持续使用技术创新的本质,深化用户持续使用行为理论,促进知识问答社区和用户需求理论的发展。[方法/过程]笔者以“期望—确认”理论模型（ECM-ISC）为基础,结合关系维持双元理论等构建在线知识问答社区的用户持续使用模型和测量量表,共搜集358份有效样本数据,采用结构方程模型进行假设检验。[结果/结论]结果表明,用户的期望确认度由信息、社交、情感、娱乐以及社会认同期望5个维度构成,5种确认度都会正面显著影响用户的满意度和感知有用性;满意度和感知有用性对用户持续使用意愿有积极影响;用户感知转换成本、用户习惯和自我效能正向影响持续使用意愿;自我效能正向影响社会认同确认度。     

Genome Mining of α-Pyrone Natural Products from Ascidian-Derived Fungus Amphichorda felina SYSU-MS7908

Culturing ascidian-derived fungus Amphichorda felina SYSU-MS7908 under standard laboratory conditions mainly yielded meroterpenoid, and nonribosomal peptide-type natural products. We sequenced the genome of Amphichorda felina SYSU-MS7908 and found 56 biosynthetic gene clusters (BGCs) after bioinformatics analysis, suggesting that the majority of those BGCSs are silent. Here we report our genome mining effort on one cryptic BGC by heterologous expression in Aspergillus oryzae NSAR1, and the identification of two new α-pyrone derivatives, amphichopyrone A (1) and B (2), along with a known compound, udagawanone A (3). Anti-inflammatory activities were performed, and amphichopyrone A (1) and B (2) displayed potent anti-inflammatory activity by inhibiting nitric oxide (NO) production in RAW264.7 cells with IC₅₀ values 18.09 ± 4.83 and 7.18 ± 0.93 μM, respectively.     

珠海市凤凰山药用植物资源多样性研究

根据第4次全国中药资源普查的技术规范与要求,采用样地与样线调查法,对珠海市凤凰山野生药用植物资源的科属组成、区系特征、生活型、药用部位、药性、药味、药效等方面进行研究.结果显示:该地区野生药用维管植物共有106科276属385种;药用植物属的分布区类型以热带性质为主;根及根茎类药物是该地药用植物的主要组成部分,占总种数...     

67例下叶基底段肺结核误诊原因分析

目的探讨下叶基底段肺结核误诊的原因,以提高诊断水平,减少误诊率．方法对珠海地区2005年1月～2008年12月登记管理的下叶基底段肺结核67例误诊病例进行回顾性分析．结果误诊为肺炎16例,支气管肺炎31例,肺脓疡8例,肺癌9例,支气管扩张并感染3例．结论下叶基底段肺结核的临床症状、X线表现均不典型,易误诊,应提高对下叶基底段肺结核的认识．全面收集临床资料,充分利用各种检查手段,寻求病原和组织学诊断依据是减少误诊的关键．     

珠海园林绿化节水对策研究

通过对珠海市园林绿化用水特点的分析。探讨了园林绿化节水途径,提出了珠海市实施节水园林绿化的一些策略。     

花鲈源杀鱼爱德华菌耐药谱及毒力相关性分析

从广东省珠海市养殖花鲈（基因鉴定出87株杀鱼爱德华菌（）。耐药谱分析显示,杀鱼爱德华菌对利福平（98.85%）、麦迪霉素（96.55%）、红霉素（95.40%）、青霉素（68.96%）、磺胺异恶唑（58.62%）、复方新诺明（28.73%）、阿莫西林（21.83%）、庆大霉素（13.79%）、新霉素（10.34%）、呋喃唑酮（3.45%）、诺氟沙星（2.29%）、氯霉素（2.29%）、多西环素（2.29%）、土霉素（1.15%）、氟苯尼考（1.14%）、恩诺沙星（0%）耐药。杀鱼爱德华菌共有32种耐药谱型且均为多重耐药菌株,多重耐药指数为0.423。斑马鱼致死率结果发现,杀鱼爱德华菌是一株中高毒力菌株;进一步的相关性分析揭示,杀鱼爱德华菌毒力与庆大霉素抗性呈正相关（<0.01）呈负相关。综上所述,花鲈源杀鱼爱德华菌为高毒、多重耐药的菌种,其毒力与耐药性多呈现负相关,推测是由于细菌因获得外源DNA而产生额外的生物成本所致。     

Mangroves of China: a brief review

The distribution, ecology, conservation and management of Chinese mangroves are reviewed. Mangroves naturally occur along the southeast Chinese coast and traverse the provinces of Hainan, Guangdong, Guangxi, Fujian and Taiwan, intermittently extending from 18°N. Thirty-seven mangrove tree species, representing 20 families and 25 genera, have been documented, with thermophilic eurytopic species being the dominant components. A remarkable decrease of species richness is evident from Hainan (18–20°N) to Fujian (23.5–27°N) (35 vs. 9 species). The existing mangrove area is ≈ 17 800 ha, accounting for slightly more than 0.1% of the world's total. Nearly two-thirds of China's mangroves have been lost during the past 40 years, largely due to conversion for rice-farming, embankment for aquaculture ponds and, recently, rapid urban development. A total of 201 papers on Chinese mangroves was published between 1950 and 1995, 178 of which are in Chinese; thus, they are not easily accessible to the international scientific community. Most of the work was conducted after 1985 (91% of the papers published) and research emphasized floristics with little attention to managementrelated issues. The net primary production of the Chinese mangroves shows a latitudinal trend, also significant deviations from predictions on models generated using non-Chinese data. Although 28 Chinese institutions have dealt with mangrove research, only five maintain long-term projects. The bulk of research has been carried out in six mangrove reserves: Qinglan, Dongzhai (Hainan), Mai Po (Hong Kong), Futian (Guangdong), Shankou (Guangxi) and Jiulongjiang (Fujian). Twelve mangrove reserves have been established so far in mainland China, one in Hong Kong (Mai Po) and one in Taiwan (Tanshui). These reserves cover an area of over 19000 ha, of which 8445 ha are mangroves (47% of existing mangrove area). Six measures that can facilitate mangrove conservation and management are recommended: (a) declare more mangrove areas as nature reserves; (b) set up a national mangrove committee and mangrove research centre to foster research and management; (c) develop concrete management guidelines; (d) enact protective legislation and ensure its strict enforcement; (e) launch education programmes in the major mangrove reserves; and (f) stop further nonsustainable exploitation of mangroves and their habitats.     

猪繁殖与呼吸综合征RT-LAMP检测方法研究

应用反转录环介导等温扩增技术(RT-LAMP),利用猪繁殖与呼吸综合征病毒(PRRSV)核衣壳蛋白(N蛋白)ORF7基因保守序列的6个区域设计2对特异引物,经反应体系、反应条件优化及敏感性和特异性试验,建立了PRRSV RT-LAMP检测方法。研究结果表明,该检测方法不需要复杂仪器,仅用一台普通恒温水浴锅,在等温条件(63 ℃)保温1 h,即可完成核酸扩增反应,加入核酸染料SYBR GreenⅠ后,用肉眼即可对试验结果进行准确判定,为田间和基层部门检测猪繁殖与呼吸综合征病毒提供了一种廉价、简便、快速、敏感、特异的新方法。