花鲈源杀鱼爱德华菌耐药谱及毒力相关性分析

从广东省珠海市养殖花鲈（基因鉴定出87株杀鱼爱德华菌（）。耐药谱分析显示,杀鱼爱德华菌对利福平（98.85%）、麦迪霉素（96.55%）、红霉素（95.40%）、青霉素（68.96%）、磺胺异恶唑（58.62%）、复方新诺明（28.73%）、阿莫西林（21.83%）、庆大霉素（13.79%）、新霉素（10.34%）、呋喃唑酮（3.45%）、诺氟沙星（2.29%）、氯霉素（2.29%）、多西环素（2.29%）、土霉素（1.15%）、氟苯尼考（1.14%）、恩诺沙星（0%）耐药。杀鱼爱德华菌共有32种耐药谱型且均为多重耐药菌株,多重耐药指数为0.423。斑马鱼致死率结果发现,杀鱼爱德华菌是一株中高毒力菌株;进一步的相关性分析揭示,杀鱼爱德华菌毒力与庆大霉素抗性呈正相关（<0.01）呈负相关。综上所述,花鲈源杀鱼爱德华菌为高毒、多重耐药的菌种,其毒力与耐药性多呈现负相关,推测是由于细菌因获得外源DNA而产生额外的生物成本所致。     

Expression of MMP3 in subchondral bones in early experimental osteoarthritis

AIM: To demonstrate that matrix metalloproteinase 3(MMP3)plays MMP3 play an important role in the pathogenesis of osteoarthritis by studying the expression of MMP3 in subchondral bones in early experimental osteoarthritis at gene and protein levels. METHODS: The SD rats were randomly divided into 2 groups: experimental group and control group, each containing 30 rats with similar body weight. The right knee joints of the rats in experimental group underwent surgery, which involved in both medial collateral ligament (MCL) transection and medial meniscectomy, while the animals in control group were only carried out a sham operation. The rats were killed at the 1st, 2nd and 4th weeks post-surgery to obtain the right knee joints. Pathological analysis was performed to validate this early osteoarthritic rat model. The expression of MMP3 in subchondral bones at mRNA and protein levels was evaluated by real-time polymerase chain reaction and immunohistochemical staining, respectively. RESULTS: The expression of MMP3 in subchondral bones was significantly increased at both the 1st and 2nd weeks post-surgery in experimental group, with the fold changes of 8.34 (P<0.05) and 4.85 (P<0.05), respectively, as compared with control group. No differentially expression of MMP3 was observed at the 4th week post-surgery between these 2 groups. A lot of MMP3 positive cells, including small mononuclear cells and bigger polynuclear giant cells, were found in subchondral bones in experimental group at the 1st and 2nd weeks post-surgery,but not in control group. At the 4th week post-surgery, no MMP3 positive cells were found in subchondral bones of both groups. CONCLUSION: MMP3 plays an important role in the pathogenesis of early experimental osteoarthritis.     

辣椒转录组SNP挖掘及多态性分析

 利用SNP分析软件从辣椒（Capsicum annuum L.）251 068条Unigenes中筛选出18 159个SNP,其中有1 781个SNP位点被匹配在1 291个注释基因上,基因功能分类和代谢途径分析表明,其中有853个基因参与初生代谢（28.7%）、细胞代谢（17.3%）、生物合成过程（15.7%）,另有125个（9.7%）基因序列参与新陈代谢途径,53条（4.1%）序列参与次生代谢产物合成途径,31条（2.4%）序列参与植物激素合成途径。 EST-SNP序列中4 172条（22.9%）满足设计CAPS引物条件,为了验证EST-SNP正确性,并选取了15对CAPS引物对5份辣椒材料进行扩增,结果发现有8对（53.3%）引物表现出多态性。表明筛选出这些EST-SNP标记可作为辣椒基因分型、图谱构建等的候选分子标记。     

基于ANSYS Workbench的S型松土铲模态分析

在分析了S型松土铲结构特点与工作过程的基础上,利用三维CAD软件Pro/E,绘制S型松土铲的实体模型,通过有限元分析软件ANSYS Workbench对S型松土铲进行了模态分析,得到了S型松土铲的前6阶模态振型图,分析的结果为优化S型松土铲的结构和改善性能提供了理论参考。     

Seagrass (Zostera marina) promotes nitrification potential and selects specific ammonia oxidizers in coastal sediments

Journal of Soils and Sediments - Seagrasses accelerate sedimentation, release oxygen and organic matter through their roots, and compete with ammonia oxidizers for ammonia/ammonium in surface...     

珠海园林绿化节水对策研究

通过对珠海市园林绿化用水特点的分析。探讨了园林绿化节水途径,提出了珠海市实施节水园林绿化的一些策略。     

Construction of shRNA targeting AGR2 gene and effect of AGR2 on biological function of nasopharyngeal carcinoma cells

AIM: To construct the shRNA targeting anterior gradient protein 2 (AGR2) gene for exploring the effect of AGR2 on the biological behavior of nasopharyngeal carcinoma (NPC) cells.METHODS: The expression of AGR2 at mRNA and protein levels in NPC cell lines 6-10B and 5-8F was detected by real-time PCR and Western blot. The pSR-GFP/Neo-AGR2-shRNA expression vector targeting AGR2 was constructed. Based on the interference targeting AGR2, the cell migration and motility were determined by Transwell migration and motility assays.RESULTS: The expression of AGR2 was increased in NPC cell line 5-8F compared with NPC cell line 6-10B (P<0.05). When the AGR2 expression in 5-8F cells was interfered, the cell migration, invasion and tumorigenicity were weakened.CONCLUSION: The expression of AGR2 is up-regulated in NPC cell line 5-8F. pSR-GFP/Neo-CLU-shRNA successfully inhibits the expression of AGR2 in NPC cell line 5-8F. AGR2 inhibits the migration, invasion and tumorigenicity of 5-8F cells in vivo.     

云南山地城镇气温空间分异的影响因素分析

以云南省不同流域内典型山地城镇为分析区域,通过消除和减弱海拔、纬度因素对多年平均气温场的影响后,在同一海拔、纬度面的典型山地城镇气温场中,基于自然地理和社会经济数据,以普通最小二乘法（OLS）建立影响因素与气温分布的全局空间回归模型,以地理加权法（GWR）建立局部空间回归模型。结果表明：对山地城镇气温具有显著性影响的因素分别为城镇GDP、地形隆起高度、流域开口角度、植被指数、第三产业比值,OLS模型调整后R²可达0.789,GWR建立的局部空间回归模型更具可靠性;以预留小城镇为检验样本,对气温模型进行精度检验,消除海拔纬度方案平均绝对误差为1.09 ℃,均方根误差为1.35 ℃,消除海拔纬度的方案较单一消除海拔纬度、保留海拔纬度的方案对气温值具有更好的精度误差。研究结果反映了山地城镇自然地理格局以及人类社会活动对气温场的分布具有重要的影响。     

湖南烈士公园一、二期景观改造设计

对于老公园的改造是公园设计的难点也是重点.文章围绕设计中要"尊重历史,善待资源"去整合现有的空间,因地制宜地结合现有环境去设计,并通过具体改造方案来阐述。