原发性肾病综合征患者血、尿γ-干扰素的检测及其临床意义

目的：为进一步阐明γ—干扰素(IFN—γ)在原发性肾病综合征(PNS)发生发展中的作用及检测IFN—γ的临床诊断价值。方法：对32例PNS患者及10例正常人血清、尿液IFN—γ的水平进行检测，并结合PNS病理类型及其他临床指标进行分析。结果：系膜增生性肾炎(MesPGN)患者尿IFN—γ水平显著高于正常对照组，而非增生性肾炎组却与对照组差异无显著性；其次，尿IFN—γ水平与尿纤维蛋白(原)降解产物(FDP)浓度呈正相关；但与血肌酐(Ser)、血清肌酐清除率(Ccr)、24h尿蛋白定量(24hUPQ)无相关性。血清IFN—γ水平各组问差别无统计学意义。结论：IFN—γ参与增生型PNS免疫性肾损伤过程，并可能通过局部方式起作用，尿IFN—γ检测有可能作为区分增生性肾炎和非增生性肾炎的指标。     

Experience with Cyclosporin-A After Renal Allografting in Two Dogs Research Note

After renal allografting, cyclosporin-A was administered to one partially nonmatched dog that was followed for 79 days. Cyclosporin-A and prednisolone were administered to one nonmatched dog that was followed for 805 days. Side effects encountered with cyclosporin-A included lymphocytic dermatitis, papillomatosis, bacterial and fungal infections, and B lymphocyte hyperplasia.     

Response of Mustard to Ethrel Spray and Basal and Foliar Application of Nitrogen

In a field trial conducted during 1992–93 and 1993–94, the effect of basal (B) nitrogen (N) (45 and 60 kg N ha⁻¹) and foliar application (F) of water (W) or 10 kg N ha⁻¹ and 400 or 600 ppm ethrel (E) (2-Chloro ethyl phosphonic acid) at 70 days after sowing was studied on leaf area index and dry mass at 90 days and pod number per plant, seeds per pod, 1000 seed weight, seed yield, oil content and oil vield at harvest of mustard ( Brassica juncea L. Czern & Coss.) cv. T-59. Recommended basal (B) application of 90 kg N ha (BN₉₀) was used as control. On the basis of 2 year data it was found that basal application of 60 kg N and foliar spray of 10 kg N ha ⁻¹ and 600 ppm ethrel gave higher values for growth and yield characteristics and enhanced seed yield and oil yield by 12.5 and 14.8%, respectively over control BN_90.     

赖氨酸占蛋白质及饲粮的比例对杜洛克猪生长肥育性能及胴体品质的影响

杜洛克猪48头,体重27kg左右,分6组。试验前期(27～60kg)日粮的消化能均为13.67MJ)/kg,后期(60～90kg)均为13.44MJ/kg。粗蛋白1、2、3组前期均为18％,后期均为16％;4、5、6组前期均为16％,后期均为14％。同时配合3种赖蛋比水平6.6～6.9％,6.1～6.25％及5％。试验结果表明:4、5、6组平均日增重、饲料利用率及胴体瘦肉率都略高于1、2、3组,但差异不显著(P>0.05)。在前期,以第5组平均日增重最高(达782克),极显著高于其他各组(P<0.01);饲料利用率2.41,极显著好于其他各组(P<0.01)。后期以第5组平均日增重最高(875克)。试验前期,第5组获得最佳的日增重(822克),极显著高于其他各组(P<0.01),饲料利用率2.98,胴体瘦肉率65.79％,与其他各组差异不显著(P>0.05)。在两种粗蛋白水平组内,均以赖蛋比6.1～6.25％的第5组和第2组生长速度最快(822克和778克)。说明在相同的能量水平下,赖蛋比是影响生长速度的主要因素。     

高寒草甸退化草地—“黑土滩”植物量测定

依据高寒草甸草地秃斑地的多少和距居民点的远近划分为轻度退化草地(极少量秃斑地)、中度退化草地(少量秃斑地)、重度退化草地(大量秃斑地)和极度退化草地(全部秃斑地)四个退化草地等级。对各等级退化草地进行了植物量测定。结果表明:草地植物量(地上和地下)及土壤含水量,随着草地退化程度的加剧明显下降(P<0.01),毒杂草量则明显上升(P<0.01)。其中极度退化草地较轻度退化草地植被中嵩草属牧草优势度减少38,可食鲜草量减少219.2g/m~2,而毒杂草则增加99.6g/m~2,植物活根量(干物质)减少2390.6g/m~2,土壤含水量(0—10cm)减少10.02％,并对高寒草甸退化草地(黑土滩)的成因及治理途径提出了看法和意见。     

Effect of Integration of Fertilizer and Green Manure Nitrogen on Yield Attributes, Nitrogen Uptake and Yield of Lowland Rice (Oryza sativa L.)

Field experiments were conducted at Tamil Nadu Rice Research Institute, Aduthurai, India, during the wet seasons of 1992 and 1993 to study the effect of full and partial substitution of fertiliser N with green manure N (Sesbania rostrata) on nitrogen uptake, yield attributes and yield of rice. The experiment consisted of eight treatments with two levels of N (100 and 200 kg ha⁻¹) and three sources of N application viz., fertilizer, integrated (1:1 fertilizer and green manure N) and green manure N compared to the recommended practice (150 kg fertilizer plus 6.25 t ha⁻¹ (72 kg N) green manure) and a no N control. Nitrogen application markedly increased the N uptake. Combined use of the two N sources at 200 and 222 kg N ha⁻¹ and of single fertilizer N at 200 kg N ha⁻¹ recorded the maximum N uptake, increased the yield attributes such as number of panicles per unit area, weight per panicle, number of total and filled grains per panicle and test weight. At 200 kg N ha⁻¹ full substitution of N by green manure reduced the grain yield but only partial substitution of N by green manure resulted in almost similar yield as single fertilizer N. Thus 200 kg N ha⁻¹ applied in equal proportions of fertilizer and green manure N can be recommended for medium duration rice cultivars.     

In vitro viability of cryopreserved equine embryos following different freezing protocols.

The main objective of this study was to evaluate two freezing protocols and the effect of agar embedding on survival of day 6.5 equine embryos. A total of 133 embryos were used, in one group (n = 51), embryos were first embedded in agar before the freezing protocol was started. A freezing protocol to -30 degrees C or -33 degrees C was used before plunging embryos into liquid nitrogen (LN2). The embryos were thawed in water at 37 degrees C, evaluated and placed in culture. After 24 h culture, the embryos were evaluated for their morphology and development. No differences were observed between embryos plunged at -30 degrees or at -33 degrees C in LN2. The analysis of the morphology and development after thawing showed that the diameter and developmental stage at freezing correlated with embryo survival. Morula and early blastocyst stages of development were associated with better quality after freezing and thawing and had a better potential to survive after in vitro culture (p < 0.05) compared to more advanced stages. The agar failed to protect embryos from zona pellucida damage, but a tendency to prevent rupture was observed in larger embedded embryos.     

奥芬哒唑干混悬剂驱除猪蛔虫的效果观察

应用奥芬哒唑干混悬剂给猪口服驱虫，设5mg/kg b．w，l0mg/kg b．w，15mg／kg b．w3个剂量试验组和对照组。结果：5mg/kg剖量组的虫卵转阴率为60％，减少率为99．40％；l0mg/kg和15mg/kg剂量组虫卵转阴率和减少率均达100%。全部给药猪均未出现毒副反应。