全文获取类型
收费全文 | 11413篇 |
免费 | 802篇 |
专业分类
林业 | 1166篇 |
农学 | 421篇 |
基础科学 | 42篇 |
2607篇 | |
综合类 | 737篇 |
农作物 | 477篇 |
水产渔业 | 781篇 |
畜牧兽医 | 4614篇 |
园艺 | 281篇 |
植物保护 | 1089篇 |
出版年
2023年 | 102篇 |
2022年 | 126篇 |
2021年 | 234篇 |
2020年 | 224篇 |
2019年 | 152篇 |
2018年 | 550篇 |
2017年 | 524篇 |
2016年 | 467篇 |
2015年 | 374篇 |
2014年 | 425篇 |
2013年 | 653篇 |
2012年 | 906篇 |
2011年 | 821篇 |
2010年 | 443篇 |
2009年 | 348篇 |
2008年 | 712篇 |
2007年 | 662篇 |
2006年 | 536篇 |
2005年 | 559篇 |
2004年 | 513篇 |
2003年 | 473篇 |
2002年 | 383篇 |
2001年 | 278篇 |
2000年 | 246篇 |
1999年 | 217篇 |
1998年 | 66篇 |
1997年 | 56篇 |
1996年 | 44篇 |
1995年 | 60篇 |
1994年 | 38篇 |
1993年 | 33篇 |
1992年 | 79篇 |
1991年 | 67篇 |
1990年 | 63篇 |
1989年 | 58篇 |
1988年 | 56篇 |
1987年 | 46篇 |
1986年 | 47篇 |
1985年 | 46篇 |
1984年 | 43篇 |
1983年 | 36篇 |
1979年 | 40篇 |
1978年 | 21篇 |
1974年 | 28篇 |
1973年 | 22篇 |
1972年 | 24篇 |
1971年 | 24篇 |
1970年 | 29篇 |
1969年 | 25篇 |
1968年 | 25篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
31.
Astruc T Marinova P Labas R Gatellier P Santé-Lhoutellier V 《Journal of agricultural and food chemistry》2007,55(23):9554-9558
In meat, no detailed studies on the intracellular distribution of oxidized proteins during oxidative stress have been performed, to our knowledge. Therefore, we used fluorescence microscopy to detect and locate protein carbonyls, oxidation products of basic amino acids, generated in bovine M. Rectus abdominis during either exposition to a chemical free radical generating system, or refrigerated storage, or cooking. The technique consisted of an immunohistochemical detection of carbonyls by reaction with the specific probe DNPH (2,4-dinitrophenylhydrazine) followed by the sequential addition of a first antibody against DNPH-carbonylated proteins and a CY3-labeled secondary antibody. The fluorescence of the CY3 probe increased regularly with level of free radical generating system and storage time. Moreover, an important heterogeneity of carbonyl distribution was observed, with a higher oxidation level at the periphery than inside the muscle cells. Cooking induced fluorescence increase only at the periphery of cells. Specific coloration of collagen by Sirius red showed that collagen was not involved in fluorescence. We can deduce that accumulation of oxidized proteins observed in the cell periphery was linked to membrane protein oxidation and not to connective tissue oxidation. Biochemical assays were performed in parallel on membrane and myofibrillar proteins to provide complementary quantitative data on level of oxidized proteins. 相似文献
32.
Rezzi S Giani I Héberger K Axelson DE Moretti VM Reniero F Guillou C 《Journal of agricultural and food chemistry》2007,55(24):9963-9968
The combination of (1)H NMR fingerprinting of lipids from gilthead sea bream (Sparus aurata) with nonsupervised and supervised multivariate analysis was applied to differentiate wild and farmed fish and to classify farmed specimen according to their areas of production belonging to the Mediterranean basin. Principal component analysis (PCA) applied on processed (1)H NMR profiles made a clear distinction between wild and farmed samples. Linear discriminant analysis (LDA) allowed classification of samples according to the geographic origin, as well as for the wild and farmed status using both PCA scores and NMR data as variables. Variable selection for LDA was achieved with forward selection (stepwise) with a predefined 5% error level. The methods allowed the classification of 100% of the samples according to their wild and farmed status and 85-97% to geographic origin. Probabilistic neural network (PNN) analyses provided complementary means for the successful discrimination among classes investigated. 相似文献
33.
Proanthocyanidin polymers, oligomers, and the structurally related monomer (+)-catechin were labeled by incorporation of radioactive precursors in shoots of willow tree (Salix caprea L.). [1-(14)C]-Acetate and [U-(14)C]-phenylalanine precursors were fed through the cut stems or petioles of leaves. Optimization of several parameters such as the nature and origin of the plant material, leaf maturity, nature, and quantity of radioactive precursor applied and the duration of metabolism led to incorporation yields of 3.2% and to specific activities of 500 microCi/g. Detailed characterization of the products (polymerization degree, procyanidin/prodelphinidin ratio, specific activities) and purification by chromatography are reported. Some sugars bound to radiolabeled proanthocyanidin polymers were removed by enzymic treatment with a mixture of glycosidases. A radioactive purity close to 100% and specific activities suitable for bioavailability studies were obtained. 相似文献
34.
1-methylcyclopropene increases storability and shelf life in climacteric and nonclimacteric plums 总被引:2,自引:0,他引:2
Martínez-Romero D Dupille E Guillén F Valverde JM Serrano M Valero D 《Journal of agricultural and food chemistry》2003,51(16):4680-4686
The effect of 1-methylcyclopropene (1-MCP) at three different doses (0.25, 0.50, and 0.75 microL L(-1)) on the ripening processes of a climacteric, cv. Santa Rosa, and a suppressed climacteric type, cv. Golden Japan, plum was studied. For both cultivars, positive effects were observed in terms of inhibition of ethylene production and delays of the physical, chemical, and biochemical changes associated with ripening. 1-MCP-treated plums were firmer with lower weight loss, reduced degrees Brix/titratable acidity ratios, and lower color changes during cold storage and subsequent shelf life at 20 degrees C than controls. For most factors, the effectiveness of 1-MCP was dose-dependent in Santa Rosa but dose-independent for Golden Japan. 相似文献
35.
Boulanger Yan Arseneault Dominique Boucher Yan Gauthier Sylvie Cyr Dominic Taylor Anthony R. Price David T. Dupuis Sébastien 《Landscape Ecology》2019,34(1):159-174
Landscape Ecology - Forest landscapes at the boreal–temperate ecotone have been extensively altered. Reducing the gap between current and presettlement forest conditions through... 相似文献
36.
Baudron Frédéric Schultner Jannik Duriaux Jean-Yves Gergel Sarah E. Sunderland Terry 《Landscape Ecology》2019,34(2):341-356
Landscape Ecology - It remains unclear how agricultural landscapes can best serve multiple purposes such as simultaneously maintaining agricultural productivity and conserving biodiversity. Our... 相似文献
37.
Pablo M. Vergara Luis O. Meneses Audrey A. Grez Madelaine S. Quiroz Gerardo E. Soto Christian G. Pérez-Hernández Paola A. Diaz Ingo J. Hahn Andrés Fierro 《Landscape Ecology》2017,32(2):279-293
Context
Interactions between landscape-scale processes and fine-grained habitat heterogeneity are usually invoked to explain species occupancy in fragmented landscapes. In variegated landscapes, however, organisms face continuous variation in micro-habitat features, which makes necessary to consider ecologically meaningful estimates of habitat quality at different spatial scales.Objectives
We evaluated the spatial scales at which forest cover and tree quality make the greatest contribution to the occupancy of the long-horned beetle Microplophorus magellanicus (Coleoptera: Cerambycidae) in a variegated forest landscape.Methods
We used averaged data of tree quality (as derived from remote sensing estimates of the decay stage of single trees) and spatially independent pheromone-baited traps to model the occurrence probability as a function of multiple cross-scale combinations between forest cover and tree quality (with scales ranging between 50 and 400 m).Results
Model support and performance increased monotonically with the increasing scale at which tree quality was measured. Forest cover was not significant, and did not exhibit scale-specific effects on the occurrence probability of M. magellanicus. The interactive effect between tree quality and forest cover was stronger than the independent (additive) effects of tree quality and particularly forest cover. Significant interactions included tree quality measured at spatial scales ≥200 m, but cross-scale interactions occurred only in four of the seven best-supported models.Conclusions
M. magellanicus respond to the high-quality trees available in the landscape rather than to the amount of forest per se. Conservation of viable metapopulations of M. magellanicus should consider the quality of trees at spatial scales >200 m.38.
de Almeida Márcia Rodrigues Schwambach Joséli Silveira Vanildo Heringer Angelo Schuabb Fett Janette Palma Fett-Neto Arthur G. 《New Forests》2020,51(2):213-241
New Forests - Adventitious rooting (AR) is an obligatory step for vegetative propagation of commercial woody species. Paper industries have interest in Eucalyptus globulus Labill and its hybrids... 相似文献
39.
Morantes César Fernández Yarza Florencia Montes María L. Mercader Roberto C. Curutchet Gustavo Torres Sánchez Rosa M. 《Water, air, and soil pollution》2020,231(1):1-13
Water, Air, & Soil Pollution - The treatment efficiency of Chlorella sorokiniana and Scenedesmus species, immobilized in sodium alginate, was evaluated for removing nitrate from groundwater.... 相似文献
40.
Spontaneous electrical signals in the retina's photoreceptors impose a limit on visual sensitivity. Their origin is attributed to a thermal, rather than photochemical, activation of the transduction cascade. Although the mechanism of such a process is under debate, the observation of a relationship between the maximum absorption wavelength (λ(max)) and the thermal activation kinetic constant (k) of different visual pigments (the Barlow correlation) indicates that the thermal and photochemical activations are related. Here we show that a quantum chemical model of the bovine rod pigment provides a molecular-level understanding of the Barlow correlation. The transition state mediating thermal activation has the same electronic structure as the photoreceptor excited state, thus creating a direct link between λ(max) and k. Such a link appears to be the manifestation of intrinsic chromophore features associated with the existence of a conical intersection between its ground and excited states. 相似文献