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71.
To investigate the neuropathogenesis of feline immunodeficiency virus (FIV) infection in vitro, we have utilized three populations of cultured feline neural cells (astrocytes, microglia, brain endothelium) to assess the relative susceptibility to FIV infection, ability to produce viral antigens, and effects of infection on cell survival. Astrocytes appeared to be the most susceptible to infection, followed by microglia, whereas brain endothelial cells were relatively resistant to infection. Astrocyte infection resulted in syncytium formation and cell death, while microglial cells remained persistently and productively infected, without obvious cytopathic effects. These results suggest that FIV entry into the central nervous system probably does not occur via infected endothelium and that both astrocytes and microglia are more likely target cells for the virus. 相似文献
72.
用免疫荧光、酶标技术检测赭曲霉毒素A的研究 总被引:1,自引:0,他引:1
该项研究采用本课题组提纯的赭曲霉毒素A(Ochratoxin A·简称OA)和研制的兔抗OA抗体等,进行间接荧光法和ELISA间接法检测OA的试验。通过对不同浓度的OA纯品和含毒饲料浸提液及中毒死亡鸡内脏的间接荧光染色,均能检出分布均匀、大小有别的黄绿色荧光亮点;用OA为被检抗原和兔抗OA抗体与羊抗兔IgG-HRP进行ELISA间接法试验,对含不同浓度OA的试验孔和对照孔的检测,结果均正确稳定。研究结果证明,以上两种检测方法检测OA均具有灵敏、特异、快速等优点。 相似文献
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75.
本言语报道昆明小哨和宜良良竹山两地区两种植被类型和两种成土母质发育的土壤上,干巴菌只能在弱酸性的沉积岩类(石灰岩或砂页岩)或河流沉积物类成土母质发育形成的山原红壤的针叶林型的云南松或阔叶林型的麻栎林下生长发育。而其它成土母质形成的土壤中云南松林或麻栎林下,不会长出干巴菌。(2)干巴菌根际土壤必须具有良好的理化性质--土壤疏松多孔、透水透气和土温传递优良;PH值在4.5-5.5区间内;土质富含钙和铁 相似文献
76.
576只190日龄蛋用父母代海兰种鸡随机分为3组,分别在常规料供钙、傍晚额外加喂供钙和早晨及下午各供钙一次的三种方式下饲养8周。结果表明:早晨和下午各一次的供钙方式提高了蛋鸡的生产性能,较常规料供钙方式和傍晚额外加喂钙方式蛋重分别提高3.503%(P<0.01)和3.658%(P<0.01),产蛋量提高3.185%(P<0.01)和3.992%(P<0.01),饲料报酬提高4.485%(P<0.01)和5.298%(P<0.01)。大幅度降低了不合格蛋比例,和常规料供钙方式差异显著(P<0.05),额外加喂供钙方式使鸡产沙壳蛋的比例上升,早晨和下午供钙方式显著改善了鸡蛋的蛋壳品质。 相似文献
77.
将构建的pBST2~6工程菌质粒用限制性内切酶BamHI/BglⅡ酶切,经琼脂糖凝胶电泳和电洗脱,回收147bp的目的ST1基因。随之将该基因分别重组到能有效表达K99菌毛抗原和LacZ酶的pGK99之K99基因BglⅡ位点和pUC18的BamHI位点中。通过ST1基因探针菌落原位杂交、特定酶切分析及DNA序列分析,筛选并鉴定出了理想重组子,从而构建出了能分别表达ST1融合基因产物的工程菌株pSK219和pXST1。 相似文献
78.
M A Goodwin J Brown M A Smeltzer T Girshick S L Miller T G Dickson 《Avian diseases》1992,36(2):356-358
Antibody titers for infectious bursal disease virus (IBDV), infectious bronchitis virus, Newcastle disease virus, and reovirus from chicks with chicken anemia agent (CAA) antibodies were compared with antibody titers from their CAA-antibody-negative counterparts. These comparisons were made in 396 chickens that were 1 day, 2 weeks, 8-9 weeks, 10 weeks, 17 weeks, or 29-32 weeks old. Only one serum sample was collected from any given chick or chicken. There were no significant differences between the antibody titers at any age for any antigen, with one exception: at 29-32 weeks, the IBDV titers were higher (t = 2.62, df = 142, P less than 0.01) in chickens with CAA antibody. Although not at all likely, we believe that the observation of high IBDV antibody titers in CAA-antibody-positive chicks could have been a spurious one. 相似文献
79.
Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains. 总被引:2,自引:1,他引:1 下载免费PDF全文
Monoclonal antibodies to bovine viral diarrhea virus (BVDV) were examined for binding with a large number of North American BVDV isolates and eight strains of the serologically related pestivirus, hog cholera virus (HCV). No single BVDV monoclonal antibody reacted with all BVDV isolates. The most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) North American isolates. From a fewer number of isolates tested, one anti-gp53 monoclonal antibody also showed a high cross-reactivity (94%). All BVDV isolates showed a positive reaction with at least one of the seven monoclonal antibodies in the panel. Thus, the results indicated that a pool of these monoclonal antibodies may be used in place of polyclonal antisera for the detection of BVDV contamination of cell lines or for virus isolation. For HCV, all three anti-p80/p125 monoclonal antibodies reacted positively with all eight virus strains. In contrast, none of the anti-gp53 monoclonal antibodies were reactive to HCV strains. Thus, the anti-gp53 monoclonal antibodies may be useful for distinguishing between usually innocuous BVDV infections and the highly significant HCV infections in swine for foreign animal disease surveillance. 相似文献
80.
Precise localization of the needle tip during CT-guided percutaneous biopsy is considered to be a key element of a successful procedure. To ensure accuracy, the true needle tip must be differentiated from a false or simulated tip which appears when the CT slice encompasses only the shaft of an angled needle. By obtaining images of an aspiration biopsy needle inserted vertically into a phantom and then incrementally tilting the gantry, the authors were able to compare the characteristic features of the true tip to the simulated tip. The true tip was abrupt and distinct and had an adjacent flame-like low density artifact. The simulated tip was indistinct and tapered, yet still produced the adjacent artifact. We concluded that the shape and distinctness of the end portion of the needle itself, rather than the attendant artifact, were the most reliable criteria for accurate needle tip identification. 相似文献