Identification of favorable alleles in the <Emphasis Type="Italic">non</Emphasis>-<Emphasis Type="Italic">yellow coloring 1</Emphasis> gene by association mapping in maize

Association mapping was conducted to explore favorable alleles of the chlorophyll-related non-yellow coloring 1 (NYC1) gene under light and dark using an association panel of 146 maize inbred lines. A total of 14 polymorphic sites were identified to be significantly associated with at least one of the chlorophyll-related traits at the seedling stage. Four single nucleotide polymorphisms (SNPs) (S320, S2951, S3901, and S3355) from the NYC1 gene were respectively strongly associated with chlorophyll b (chlb), the ratio of chlorophyll a to chlorophyll b (chl_ratio), chlorophyll a degradation (chla_deg), and total chlorophyll degradation (total_chl_deg). SNPs S320 (C/A) in exon 1, and S2951 (A/G) in intron 8 was related to chlb, with 6.01 and 8.89% of phenotypic variation under light treatment, respectively. Under dark treatment, SNP S3901 (C/T), located in 3′ untranslated region (3′UTR), was associated with chl_ratio, explaining 7.01% of the observed phenotypic variation, whereas SNP S3355 (C/G) in intron 9 explained 6.48 and 5.18% of phenotypic variations in chla_deg and total_chl_deg, respectively. Taken together, these results indicated that the NYC1 gene plays an important role in chlorophyll content and other related traits, and different sites act on chlorophyll metabolism under different light intensities in maize seedlings. Furthermore, these findings improve our understanding of the genetic basis of chlorophyll metabolism under different light conditions.     

肥料包膜材料使用安全风险评价和控制研究

近年来,我国缓释肥料产量和消费量跃居世界前列,包膜材料的安全风险问题引起广泛关注。介绍了包膜材料的使用现状,分析了各类包膜材料安全性、人工合成高分子材料降解性以及膜材残留积累对土壤生态环境影响的安全风险;系统研究了包膜材料的生物分解能力、生态毒性等环境影响评价方法及标准指标;提出了包膜材料使用的安全风险划分等级,以期为科学评价包膜材料和合理使用包膜缓释肥料提供技术手段,降低包膜材料对农产品和生态环境的影响风险。     

新疆野生果树资源数据库系统的设计与实现

新疆的野生果树种类丰富而独特,起源古老,为中国果树栽培提供了丰富的种质资源和物质基础.因此,建立新疆野生果树资源数据库对于新疆野生果树资源的保护与研究具有十分重要的意义.笔者介绍了以Visual Basic 6.0为主导设计语言,结合后台关系数据库Access2003.建立新疆野生果树资源数据库信息系统的原理和方法,运用计算机的数据库技术开发出一套完整的可广泛应用于果树生产、科研、教学、社会服务等的野生果树种质资源数据库程序.本系统包括新疆的野苹果、野杏、野生樱桃李、野生欧洲李、野核桃等野生果树资源分布、植物学特征和农业生物学特征等数据内容.     

湖羊生长性状遗传参数的估测

对湖羊生长性状的表型参数和遗传参数的估测表明，性别对这些生长性状有极显著的影响，在遗传参数的估测中剔除性别的效应可提高估计的正确性。初生重、断奶重、三月龄重、六月龄重、周岁重和日增重等性状都属于高遗传力性状，遗传力分别为０．４１９０，０．３０４２．０．６０２３，０．６０７７，０．３９５６和０．６９４５．根据相关分析结果，为提高湖羊的生长速度和肉用性能，把日增重和三月龄重作为主要选择性状，预期可获得较好的效果     

不同施磷条件下大豆植株农艺性状与磷效率的关系

【研究目的】为了寻找直观、简便、可靠的用于磷效率筛选的农艺性状指标,探讨大豆植株农艺性状与磷效率的关系,【方法】试验选用3个“磷低效”大豆基因型HND3、HND17和HND18及“磷高效”大豆基因型HND34、HND37和HND38,采用高、低磷土壤盆栽试验,对大豆基因型磷效率与植株农艺性状的关系进行了研究。【结果】结果表明,在相关分析中,低磷（-P）和高磷（＋P）处理的x1（株高）、x4（单株荚数）、x5（单株粒数）、x8（地上部干重）和x9（根干重）,（-P）处理x3（节数）和（＋P）处理x2（分枝数）与磷效率（籽粒产量）均达到了显著或极显著水平。经逐步回归分析,建立了回归方程：（-P）处理：y=391.629＋9.271x1-2.674x2＋7.236x4＋76.932x9;（＋P）处理：y=699.882＋3.572x1＋22.956x4-191.103x6。在通径分析中,各农艺性状对籽粒产量的重要性依次为：（-P）处理：株高（0.456）〉单株荚数（0.360）〉根干重（0.267）〉分枝数（-0.021）;（＋P）处理：单株荚数（0.845）〉株高（0.126）〉每荚粒数（-0.117）。【结论】研究表明,在磷高效大豆基因型筛选中,应该考虑高、低磷条件下株高、单株荚数和根干重的综合表现。     

Oncolytic Vaccinia Virus Carrying Aphrocallistes vastus Lectin (oncoVV-AVL) Enhances Inflammatory Response in Hepatocellular Carcinoma Cells

Aphrocallistes vastus lectin (AVL) is a C-type marine lectin derived from sponges. Our previous study demonstrated that oncolytic vaccinia virus carrying AVL (oncoVV-AVL) significantly enhanced the cytotoxicity of oncoVV in cervical cancer, colorectal cancer and hepatocellular carcinoma through the activation of Ras/ERK, MAPK/ERK and PI3K/Akt signaling pathways. In this study, the inflammatory response induced by oncoVV-AVL in a hepatocellular carcinoma cell (HCC) model was investigated. The results showed that oncoVV-AVL increased the levels of inflammatory cytokines including IL-6, IL-8 and TNF-α through activating the AP-1 signaling pathway in HCC. This study provides novel insights into the utilization of lectin AVL in the field of cancer therapy.     

凋萎和添加剂对红三叶青贮的影响

为探讨不同添加剂对鲜贮或晾晒红三叶青贮的影响,以盛花期红三叶为原料,分别设计对照、添加甲酸（6mL／kg）gL蔗糖（2％）处理,袋装密封青贮360天后取样分析。结果表明,晾晒极显著提高红三叶青贮饲料的pH（P〈O．01）,降低乳酸含量（P〈0．01）。添加蔗糖和甲酸能改善红三叶青贮饲料的发酵品质。添加蔗糖和甲酸都极显著降低青贮饲料的pH以及乙酸和丙酸含量（P〈0．01）,极显著增加乳酸含量（P〈O．01）。添加甲酸可显著提高红三叶青贮饲料的干物质保存率（氏0．05）。青贮之后,硝酸盐含量下降。     

北美白云杉引种育苗试验初报

本文对引进的10个种源北美白云杉、3个种源的内陆红皮云杉3个年龄段苗木生长状况进行了分析，结果表明：各种源的苗木高生长差异显著，自云杉种源间、红皮云杉种源间及各种源之间3a生苗木地径生长差异达极显著水平；白云杉3a生苗高和地径生长表现最佳的种源是3^#、9^#和7^#，红皮云杉种源为CK2的地径及苗高生长量最大，其次是CK3和CK1，而且白云杉3^#种源苗高生长略超过红皮云杉CK3。     

黄土高原坡改梯综合效益分析——以燕沟流域为例

 退耕坡地是近年来黄土高原整治生态环境和控制水土流失的关键措施,但对于如何退耕,学术界存在多种不同观点,实践方面存在多种不同模式。以延安燕沟流域坡改梯为例,利用2005年延安站的逐日气象数据和燕沟流域地貌、土壤及土地利用等资料,借助WIN-YIELD软件,对不同地形坡度条件下坡改梯在作物增产、保水减沙效益以及燕沟流域坡改梯实践效果进行模拟、对比和实证分析。结果表明:地形坡度越大,坡改梯作物增产、保水和减沙效益越显著;坡度小于5°的坡耕地可以不进行梯田改造,坡度大于15°的坡耕地应及早实施坡改梯或退耕还林(草)。