Seroprevalence of subtype H3 influenza A virus in South Korean cats

To investigate the potential transmission of subtype H3 influenza virus to cats, a serological survey was carried out in South Korea. Serum samples (n = 1027) were obtained from 809 pet cats and 218 domesticated cats living in urban colonies (D-cats) from 2008 to 2010, and tested using an influenza anti-nucleoprotein (NP)-specific enzyme-linked immunosorbent assay (ELISA) and the haemagglutination inhibition (HI) test, which was recommended by the World Organization for Animal Health. Anti-influenza virus antibodies were detected in 3.12% and 2.43% of cat sera tested using the NP-specific ELISA and HI test, respectively. Anti-H3 antibodies were also identified when the HI assay was used for influenza virus serotyping. These data may indicate the sporadic transmission of subtype H3 influenza virus from other infected species to cats in South Korea.     

Seroprevalence of chlamydial infection in dairy cattle in Guangzhou, southern China

Chlamydia spp. are obligate intracellular gram-negative bacteria that cause a wide range of significant diseases in humans and animals worldwide, resulting in significant economic losses. Chlamydial infection in cattle has been reported in many countries including China. However, there has been no survey of chlamydial infection of dairy cattle in Guangzhou, southern China. The objective of the present investigation was to examine the chlamydial seroprevalence in dairy cattle in Guangzhou, subtropical southern China by using an indirect hemagglutination assay (IHA). The overall seroprevalence of chlamydial infection in dairy cattle was 7.25% (29/400). Greater than or equal to eight-yr-old dairy cattle had the highest seroprevalence (10.34%), followed by those that were ≥ 6 years old or < 7 years old dairy cattle (10.20%), although there were no statistically significant differences among different groups (P > 0.05). Dairy cattle with 5 pregnancies had the highest seroprevalence (10.81%). These results indicate that chlamydial infection was present in dairy cattle in Guangzhou, subtropical southern China, and integrated strategies and measures should be executed to control and prevent chlamydial infection and disease outbreak in the study region.     

赖氨酸产业生存调查：经销商的过去、现状以及未来

近10年以来．中国赖氨酸工业蓬勃发展,一些生产商也发展壮大．涌现了像大成生化等全球性主要生产商．中国赖氨酸出口由本世纪初的不足千吨水平发展到2008年的15万t左右,8年增长达到150倍以上：而国内需求也由开始的10万t左右增长至2008年的25万t以上。     

马eCG/LHβ亚基基因多态性研究

马绒毛膜促性腺激素(equine chorionic gonadotropin,eCG)是由妊娠期母马胎儿尿膜—绒毛膜细胞分泌的一种由α、β两个亚基组成的异源二聚体糖蛋白,具有FSH、LH的双重活性。采用PCR-SSCP和测序方法对28匹蒙古马和25匹纯血马的eCG/LHβ亚基基因的多态性进行研究,结果发现,在蒙古马eCG/LHβ亚基基因5′调控区存在一突变位点:G-415A分为AA、BB、AB 3种基因型,AA、BB、AB基因型频率分别为0.89、0.07、0.04;纯血马此基因中未检测到多态性。     

高寒地区小黑麦在断奶初期奶公犊瘤胃内动态降解的研究

选用3头3月龄左右装有瘤胃瘘管的荷斯坦奶公犊,按3×3完全拉丁方试验设计,喂给3种日粮,日粮组成按精粗比分别表示为:Ⅰ组70∶30、Ⅱ组60∶40、Ⅲ组50∶50,研究不同日粮组成下高寒地区小黑麦的DM、CP以及NDF在断奶初期奶公犊瘤胃内动态降解。试验结果表明:精粗比为60∶40日粮时,其CP的消失率和降解参数明显低于精粗比为70∶30和50∶50的日粮,差异显著(P<0.05);其DM和NDF的消失率和降解参数明显高于精粗比为70∶30和50∶50的日粮,差异显著(P<0.05)。     

新发现的骆驼朊病毒病

骆驼朊病毒病是近来在北非地区发现的感染单峰驼的传染性海绵状脑病。发病骆驼年龄均在8岁以上,病程3~8个月,以行为异常、共济失调、反应过敏、不时倒地、站立困难为特征;在脑部特定区域出现海绵状空泡病变,通过免疫组化和免疫印迹方法能够检测到朊病毒。世界动物卫生组织（WOAH）暂未将该病判定为新发病。由于相关信息非常有限,本文仅对该病的起源、临床症状、病理变化、病原特点、WOAH观点、防控建议等进行简要介绍,以期引起我国动物疫病防控管理部门和相关从业人员的警惕。     

东北民猪荷包猪抗性基因多态性及其与免疫指标的相关性研究

研究目的是检测荷包猪FUT 1和Mx1基因位点多态性及其与免疫指标的相关关系。采用PCR-RFLP技术分析基因多态性,采用ELISA方法检测免疫指标白介素-4(IL-4)、分泌型免疫球蛋白A(sIgA)、α-干扰素(IFN-α)的表达量。结果表明,荷包猪FUT 1基因和Mx1基因Hin6I点酶切位点上显示多态性,优势基因型分别为GG和AA型,在Mx1基因其他两位点处未发现多态性;荷包猪免疫指标IL-4、sIgA、IFN-α的表达量明显高于大白猪(P<0.05),但抗性基因型与免疫指标间的相关性表现不明显。