Association of tumor budding with tumor-infiltrating lymphocytes and prognosis in breast cancer patients

AIM: To investigate the relationship of tumor budding with clinicopathologic parameters, tumor-infiltrating lymphocytes (TILs) of tumor microenvironment and the prognosis in breast cancer patients.METHODS: A total of 178 HE section samples were collected from the breast cancer patients treated with surgery in the First Affilated Hospital of Jinan University during Jan. 2012 to Dec. 2016. The tumor budding and stromal tumor-infiltrating lymphocytes were observed under light microscope. The correlation of tumor budding with the clinicopathologic status and TILs were analyzed by χ² test. Kaplan-Meier survival analysis and Log-rank test were used to estimate the disease-free survival (DFS) and overall survival (OS).RESULTS: High tumor budding level was associated with more positive lymph nodes, higher grade, and more lymphovascular invasion. In addition, the patients with higher tumor budding level showed fewer TILs, while the patients with lower tumor budding level had more TILs. Furthermore, the patients with higher tumor budding level had a worse disease-free survival and overall survival than those with lower tumor budding level.CONCLUSION: Tumor budding is significantly associated with adverse clinicopathological characteristics of breast cancer and negatively correlated with TILs. Therefore, tumor budding may serve as a potential biomarker to predict the prognosis of breast cancer.     

Effects of puerarin on apelin-12, angiotensin II,NO and blood pressure in renovascular hypertensive rat

AIM: To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats, and to measure puerarin-induced changes of apelin-12, angiotensin II (Ang II) and nitric oxide(NO), the factors related to development of hypertension. METHODS: Sixty-five male Sprague-Dawley rats were used, of which 8 rats were randomly selected as sham operation group, and the remaining were used to make two-kidney, one-clip model. The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups: high-, middle- and low-dose puerarin groups, captopril group, and model group. The drugs were administered for 6 weeks. Blood pressure was measured every 2 weeks. Six weeks after treatment, all rats were sacrificed under deep anesthesia. Blood and kidney samples were collected. The level of apelin-12 in serum and kidneys was detected by ELISA. The level of Ang II in plasma and kidneys was measured by radioimmunoassay. NO level in serum was examined by nitrate reductase assay. RESULTS: Puerarin had an antihypertensive effect in a dose-dependent manner. Marked decreases in the level of serum apelin-12 in high- and middle-dose puerarin groups were observed(P<0.01). Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level (P<0.05). As the dose of puerarin went up, the level of apelin-12 in the kidneys was gradually decreased. Puerarin at high and middle doses obviously reduced the level of AngII in plasma, while purarin at low dose did not produce any significant effects. Puerarin at high and middle doses markedly increased the level of NO in serum, but puerarin at low dose did not induce any significant changes. CONCLUSION: Puerarin has an antihypertensive effect, and its mechanism may be related to inducing the changes of apelin, Ang II and NO, and regulating the balance among those factors.     

Statistical Model on the Time and Time Difference Error of Arrival in TDOA Location Estimation

Additional time delay of arrival resulted from non-line of sight(NLOS)propagation and muti-path effect in(cellular) environment is the dominant factor resulting in TDOA location error,study on the statistical property of TDOA error is useful for improving the accuracy of TDOA location.Based on the exponential distribution property of NLOS propagation delay,and considering the system error resulted from the signal detection,the authors establish a statistical model of arrival time and a model of TDOA error distribution by statistics when non-light of sight and multi-path propagation exist.The models reflect the statistical property of arrival time and TDOA error in cell network,the simulation shows that the proposed models have a good accuracy and a satisfactory behavior.     

新疆降水研究进展综述

目的研究转基因棉花在荒地及栽培地的生存竞争能力及环境适应性,为其环境安全性评价研究提供理论依据。方法以转KatG和SacB基因的抗旱棉花为研究对象,在荒地以及栽培地中研究转基因抗旱棉花的环境适应、生存竞争能力以及对农田生物群落的影响,结合新疆气候条件,分析转基因棉花在荒地条件下演变成杂草的可能性,利用生态学信息论方法分析种植转基因抗旱棉花对生物群落的影响。结果3个试验区域内越冬试验材料均未能出苗,荒地生存竞争试验材料出苗率较低,出苗率最高为47.3%;植株矮小,株高最高为39.8 cm;生育期内未能开花结铃。3个试验区域杂草群落的香农—维纳指数最大为2.232,最小为2.070,均匀度指数最大为0.920,最小为0.737,相同试验区域的不同棉花种植区域间均无显著性差异(P>0.05);不同播种深度下杂草群落的香农—维纳指数最大为1.603,最小为1.188,均匀度指数最大为0.874,最小为0.615,且相同棉花材料间杂草的香农—维纳指数无显著性差异(P>0.05);不同的种植密度下杂草群落的香农—维纳指数最大为1.455,最小为1.263,均匀度指数最大为0.918,最小为0.615,且相同棉花材料间杂草的香农—维纳指数无显著性差异(P>0.05)。结论转基因抗旱棉花在新疆地区荒地的环境适应性较弱,落粒棉种没有越冬的可能,演变成杂草的可能微乎其微;在荒地生态区域中与其他物种间生存竞争处于劣势,不会显著影响种植区域内的群落多样性及物种分布。     

发菜生存与生长分布的微生态环境

通过野外调查 ,研究和分析了对发菜生存与生长发育起直接作用的微土壤、微气候、微地形、微植被和风向等微生态环境。发菜对生存环境的选择性很强。其中 ,干一湿交替的微气候环境是一个起决定作用的关键性因子 ,是发菜生存和生长发育的必要条件。一方面 ,发菜藻体在干燥的状态下 ,有利于干物质的积累 ,是发菜生存和生长的必要前提 ;另一方面 ,发菜藻体只有在一定湿润状态下 ,才能生长发育。由于干旱气候环境所限 ,野生发菜的生长速度极为缓慢。     

东河早藕土壤养分限制因子试验

通过土壤养分限制因子试验,对比了不同配方施肥条件下东河早藕田间植物学性状、生育期和经济性状的差异.试验结果表明,缺氮、磷、钾肥对东河早藕的生长发育及产量形成具有明显的抑制作用,且效果由大到小顺序为氮肥、钾肥、磷肥.针对浙中地区部分土壤缺锌,补施锌肥能促进东河早藕早熟高产.     

油料树种风吹楠播种繁殖试验

对热带油料树种风吹楠的播种繁殖试验结果：风吹楠整个果实土壤播种萌发率高达90.52%,种子土壤和沙床播种萌发率分别为68.75%和64.81%,但整个果实沙床播种萌发率仅为12.64%。整个果实和种子的土壤播种苗都生长良好,生长量无明显差异。     

蒙古栎秋播种育苗技术的研究

自１９９３年起对本局大趟子苗圃进行了蒙古栎秋播育苗试验,并对蒙古栎从采种、种子调制、田间管理及起苗、留床、造林等环节上形成了一套完整的科学育苗方法,避免了春播育苗种子储藏和鼠害等一系列不利问题,研究探索了最佳播种量和最佳经营密度。     

氮磷钾养分配比对温室土培黄瓜产量及品质的影响

在日光温室内,采用盆栽方式研究了不同氮磷钾养分配比对土壤栽培黄瓜产量及其品质的影响。结果表明：增加磷素用量和比例可以显著提高黄瓜的产量;适量氮钾肥配合增施磷肥可以增加果实中可溶性蛋白含量,适量氮磷肥配合增施钾肥可以改善果实品质,提高可溶性糖和维生素C含量,降低硝酸盐含量。