冬小麦种质“矮孟牛”中新型小麦-黑麦复杂易位的遗传传递分析

本文利用矮孟牛中一种新型小麦-黑麦复杂易位IRS·7DS,1BL·7DL与1BL·1RS易位所构建的一套重组自交系(包括109个F6株系)和8个矮孟牛衍生品种进行了分析,结果表明在109个F6株系中,纯合复杂易位占69个,纯合1BL·1RS占36个,二者比例约2∶1,杂合类型占4个,出现频率4/109;对矮孟牛8个衍生品种的分析表明,6个品种包含来自矮孟牛的     

基于ZigBee的鸡舍环境无线监测系统设计与实现

为了对规模化和集约化养殖场鸡舍内部环境进行监测,针对传统的有线监测系统存在的诸多缺陷,设计了一种基于Zig Bee的鸡舍环境无线监测系统。以CC2530无线网络芯片为核心,在其外围接口集成了温度、湿度、光照、NH3、H2S和CO2六种传感器节点,搭建了无线网络,并进行数据测量与传输,通过编译底层程序和开发上位机软件,所测数据直观的呈现在用户面前。实践表明,系统运行稳定、测量数据精确、布点灵活、能耗低、成本低,适合对鸡舍环境进行精准监测。     

基于SWAT模型的西江流域径流模拟研究

采用SWAT(Soil and Water Assessment Tool)模型,在整个珠江流域构建分布式水文模型,对西江流域1951~2008年的月径流过程进行模拟。选取西江流域的迁江、柳州、武宣3个水文站的径流数据,利用SWAT模型自带的敏感性分析模块和自动率定模块,采用LH-OAT敏感性分析方法和SCE-UA算法分别对选取的各参数进行敏感性分析和参数率定,并对率定模型进行验证。敏感性分析结果表明,不同的参数对流域月径流过程表现出不同程度的敏感度,其中CN2、ESCO、SOL_AWC、CH_K2和ALPHA_BF的敏感度较高,是较敏感因子。从率定和验证结果来看,3个水文站的径流模拟效果均较好,Nash-Suttcliffe效率系数ENS均在0.75以上,其中柳州站和武宣站率定期和验证期的ENS在0.8以上。SWAT分布式水文模型在西江流域月径流模拟中的应用效果良好。     

Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell

In order to obtain the cloned sheep using bone marrow mesenchymal stem cells（BMSCs）as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son（RCP）was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment.     

Analysis on Differential Expression Genes in Porcine Aortic Vascular Endothelial Cells Induced by Apolipoprotein C Ⅲ

The aim of this study was to investigate the differential expression genes induced by ApoCⅢ,and study the function of ApoCⅢ.Porcine aortic vascular endothelial cells were successfully isolated using enzyme digestion,and then screened the differential expression genes induced by ApoCⅢ using the Solexa high-throughput sequencing technology.The results showed 647 differential expression genes,including 390 up-regulated genes and 257 down-regulated genes.The qRT-PCR results verified that the gene expression results from Solexa sequencing data were reliable.GO and Pathway analysis showed that the function of differential expression genes were related to immune response,cell apoptosis and death.These findings suggested that ApoCⅢ affected the physiological function of porcine aortic endothelial cells by the molecular pathways of inflammation,cell adhesion and apoptosis,which provided a theoretical basis for further understanding the molecular mechanisms of atherosclerosis caused by ApoCⅢ.     

一株新疆灵芝的鉴定及生长和产糖性能初探

以新疆阿勒泰采集的灵芝子实体XJ-001为试材,采用组织分离纯化获得该菌纯培养,以菌丝体基因组DNA为模板进行ITS片段扩增,测序并进行系统发育分析;以5种不同培养基培养菌株,通过比较生长速度,选出适宜母种培养基;摇瓶发酵研究不同碳氮源对菌株菌丝体生物量、胞内及胞外多糖含量的影响。结果表明:根据形态特征结合rDNA-ITS分析,初步将XJ-001鉴定为Ganoderma lucidum,并获得GenBank登录号为KT997431;菌株在玉米粉、豆饼粉琼脂培养基长势最优,生长速度为18.7mm·d~(-1);以葡萄糖、酵母浸粉为最适碳、氮源,在转速150r·min~(-1),30℃培养5d,菌株XJ-001菌丝体生物量、胞内/外多糖含量分别为8.15、1.18、0.05mg·mL~(-1)。该菌株具有生长迅速和产糖较高的潜在性能,可为开发新疆野生灵芝资源提供参考。     

铁皮石斛优质试管苗繁育技术研究

以野生铁皮石斛为试材,采用限根培养法,研究了铁皮石斛种子非共生萌发特性及优质试管苗繁育技术。结果表明:授粉120d的种子达到生理成熟,能够顺利萌发;授粉140d种子达到形态成熟,萌发率可达98.51%;低温黑暗限制保存原球茎可控制其生长;在特定容器中用限根培养基MS+0.10mg·L~(-1) 6-BA+0.3mg·L~(-1) NAA+香蕉泥80g·L~(-1)培养小苗可得到优质试管苗;把试管苗移栽到松树皮中,成活率可达97.62%,该研究为铁皮石斛产业化育苗提供了新方法。     

利用香菇菌糠栽培罗勒初探

以香菇菌糠为试材,采用不同比例的香菇菌糠和花园土、砂土的混合基质种植罗勒,测定其发芽率、形态指标以及生理指标,研究了香菇菌糠对罗勒幼苗生长的影响。结果表明:适宜种植罗勒的菌糠配方为花园土、菌糠、砂子=6∶1∶1;不同比例的菌糠配方对罗勒的发芽率有一定影响,随着菌糠比例的不断增加,罗勒的发芽率也不断提高,发芽时间也随菌糠的增加有一定缩短;该试验为菌糠的循环利用开辟了一条新的途径。     

外源乙烯和1-甲基环丙烯处理对采后胡萝卜品质的影响

以胡萝卜为试材,采用乙烯及乙烯受体抑制剂(1-甲基环丙烯)处理,通过对贮藏60d后胡萝卜的品质劣变率(长须根率、腐烂指数、出薹率)、外观指数、可溶性糖含量及多酚氧化酶活性进行测定,研究了1-MCP对胡萝卜采后贮藏品质的影响。结果表明:1-MCP结合手洗贮藏可抑制胡萝卜贮藏期间的品质劣变率、抑制可溶性糖含量的损失、保持较高的外观指数及抑制多酚氧化酶(PPO)活性。手洗后贮藏则可有效改善胡萝卜贮藏期间的表皮褐变问题。