全文获取类型
收费全文 | 7534篇 |
免费 | 46篇 |
国内免费 | 14篇 |
专业分类
林业 | 742篇 |
农学 | 240篇 |
基础科学 | 49篇 |
924篇 | |
综合类 | 335篇 |
农作物 | 508篇 |
水产渔业 | 466篇 |
畜牧兽医 | 3606篇 |
园艺 | 199篇 |
植物保护 | 525篇 |
出版年
2022年 | 12篇 |
2018年 | 121篇 |
2017年 | 184篇 |
2016年 | 169篇 |
2015年 | 237篇 |
2014年 | 204篇 |
2013年 | 165篇 |
2012年 | 451篇 |
2011年 | 307篇 |
2010年 | 108篇 |
2009年 | 78篇 |
2008年 | 63篇 |
2007年 | 42篇 |
2006年 | 44篇 |
2005年 | 781篇 |
2004年 | 653篇 |
2003年 | 448篇 |
2002年 | 113篇 |
2001年 | 134篇 |
2000年 | 34篇 |
1999年 | 149篇 |
1998年 | 10篇 |
1992年 | 117篇 |
1991年 | 145篇 |
1990年 | 168篇 |
1989年 | 147篇 |
1988年 | 172篇 |
1987年 | 113篇 |
1986年 | 143篇 |
1985年 | 118篇 |
1984年 | 91篇 |
1983年 | 81篇 |
1982年 | 33篇 |
1981年 | 17篇 |
1980年 | 23篇 |
1979年 | 133篇 |
1978年 | 96篇 |
1977年 | 91篇 |
1976年 | 77篇 |
1975年 | 137篇 |
1974年 | 129篇 |
1973年 | 116篇 |
1972年 | 138篇 |
1971年 | 118篇 |
1970年 | 88篇 |
1969年 | 102篇 |
1968年 | 113篇 |
1967年 | 125篇 |
1966年 | 102篇 |
1965年 | 70篇 |
排序方式: 共有7594条查询结果,搜索用时 15 毫秒
31.
Yuki Ichinose Rena Shimizu Yoko Ikeda Fumiko Taguchi Mizuri Marutani Takafumi Mukaihara Yoshishige Inagaki Kazuhiro Toyoda Tomonori Shiraishi 《Journal of General Plant Pathology》2003,69(4):244-249
The flagellins purified from Pseudomonas syringae pv. tabaci induce a hypersensitive reaction in nonhost tomato cells. To investigate the role of flagella and flagellin in the compatible interaction, we generated two types of flagella-defective mutant. The fliC mutant lost the fliC gene that encodes flagellin protein, whereas the fliD mutant lost the fliD gene that encodes HAP2-capping protein. The two mutants had markedly reduced ability to cause disease symptoms in tobacco leaves. Furthermore, propagation of the mutants in tobacco leaves was less than that in wild-type pv. tabaci. Compared to the inoculation with wild-type pv. tabaci, inoculation with the two mutants did not markedly induce the expression of typical defense response-related genes such as PAL and hsr203J. Complementation of each fliC and fliD gene to the corresponding deficient mutant restored motility and virulence. These results indicate that flagella of P. syringae pv. tabaci are indispensable organelles for complete virulence on host tobacco plants. 相似文献
32.
33.
Putranto HD Kusuda S Inagaki K Kumagai G Ishii-Tamura R Uziie Y Doi O 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(5):569-571
Feces were collected from two female and one male Siberian tigers, Panthera tigris altaica. Steroid hormones were extracted from lyophilized feces and quantified by enzyme immunoassay. The fecal contents of estradiol-17beta (E(2)) and testosterone in the females and male, respectively, changed markedly throughout the year. The fecal E(2) contents of females Nos. 179 and 238 increased at 26.4 +/- 8.0 and 28.0 +/- 14.2 day intervals, respectively. However, the fecal contents of progesterone (P(4)) in the female kept alone did not change. In contrast, the other female, which was kept with a male, had increased fecal P(4) contents after copulation. The fecal progesterone levels of the pregnant female remained high during her 106-day pregnancy. 相似文献
34.
Adherence of Actinobacillus pleuropneumoniae serotype 1 to swine buccal epithelial cells involves fibronectin 下载免费PDF全文
Roberto Hamer-Barrera Delfino Godínez V. Idalia Enríquez Sergio Vaca-Pacheco Rodrigo Martínez-Zú?iga Patricia Talamás-Rohana Francisco Suárez-Güemez Mireya de la Garza 《Canadian journal of veterinary research》2004,68(1):33-41
The swine pathogen Actinobacillus pleuropneumoniae serotype 1 was investigated for its ability to adhere to swine, rat, and human buccal epithelial cells (BEC). The highest number of bacteria adhered was to swine BEC. This binding ability was affected by heating, extreme pH, treatment with sodium dodecyl sulfate, ethylenediamine tetraacetate, or periodate, and proteolysis, suggesting that cell-surface glycoproteins participate in adherence and that adherence is based mostly on ionic interactions. Mannose and swine fibronectin may play a direct role in this interaction. Convalescent-phase serum from naturally infected pigs inhibited the adhesion. There was a correlation between bacterial pathogenicity as well as host specificity and the capacity for adherence to swine BEC. Adhesion to swine BEC provides a convenient method to study in vitro the adherence of A. pleuropneumoniae and other pathogens of the pig respiratory tract. 相似文献
35.
Kun?Zong Jian?Huang Kazuhide?Nara Yahua?Chen Zhenguo?Shen Chunlan?LianEmail author 《Journal of Forest Research》2015,20(6):493-500
To advance our understanding of the effects of inoculation with ectomycorrhizal fungi (EMF) on seedling colonization in mine wastelands, we conducted a field experiment in a copper tailing. Six-month-old seedlings of Japanese red pine (Pinus densiflora) and oak (Quercus variabilis) separately inoculated with three EMF species (Pisolithus sp., Cenococcum geophilum, Laccaria laccata) were transplanted to the copper tailing. The survival rates of tree seedlings were monitored monthly, and growth (biomass and height), contents of nutrients and heavy metals (K, P, Ca, Mg, Cu, Zn), and mycorrhizal infection rates of seedlings were determined 6 months after planting. Oak seedlings exhibited higher survival rates than pine seedlings after 6 months of growth on the tailing. EMF inoculations of pine seedlings significantly enhanced their survival, growth, and nutrient uptake. In contrast, EMF inoculations of oak seedlings improved growth only in terms of biomass. Additionally, EMF inoculation caused pine seedlings to accumulate more Cu and Zn in roots compared to non-inoculated seedlings, whereas inoculation inhibited the accumulation of heavy metals in shoots. However, similar results were not observed in oak seedlings. Observations of roots indicated that the rates of mycorrhizal infection of both tree species had dramatically declined at harvest time. In conclusion, ectomycorrhizal symbioses can improve the survival and performance of pine seedlings in mine tailings. The present study provided direct evidence of the importance of EMF inoculation of seedlings to the reforestation of mine wastelands. 相似文献
36.
G Flachowsky H Wilk H J L?hnert W I Ochrimenko D Geinitz A Hennig 《Archives of Animal Nutrition》1990,40(1-2):85-100
Five experiments with 18 to 36 male calves each of the black and white dairy cattle breed (age: 14-21 days, initial live weight: approximately 45 kg per animal) were carried out in order to investigate the influence of various vitamin A supply (0-80,000 IU per 100 kg LW and day) on dry matter intake and weight gain as well as the vitamin A status of liver and blood plasma over 84 days. The calves consumed a diet free of carotene and vitamin A consisting of milk replacer, concentrate and chopped wheat straw. The calves were fed in three experiments for a longer time in order to observe the further vitamin A depletion. Nine animals consumed an unsupplemented ration, nine other one got 10,000 IU vitamin A per 100 kg LW and day. Biopsies of liver and plasma samples were taken from 4 animals per group every four weeks. The various vitamin A supplementation did not significantly influence the dry matter intake (Mean: 1.67; 1.48 to 1.80 kg DM per animal and day) and the weight gain of calves (Mean: 702, 599 to 770 g per animal and day). First vitamin A deficiency symptoms (reduced feed intake, decreased weight gain, diarrhoea etc.) were observed in animals of unsupplemented group after 100 days of experiments. After 84 days the vitamin A concentration of liver of animals of unsupplemented groups decreased to 1.3-32.2% compared with the begin of experiments (60.6-155.7 mumol/kg fresh matter). Up to 51% of initial concentration were found when 10,000 IU vitamin A per 100 kg LW and day were fed. About 25,000 IU vitamin A per 100 kg LW and day were required in order to keep the initial level of vitamin A concentration of liver. The plasma vitamin A concentration is unsuitable for estimation of vitamin A status of calves. The concentration of vitamin A of liver and plasma amounted to 114 mumol per kg and 0.25 mumol per litre at the begin of experiments. The vitamin A concentration of liver of unsupplemented group decreased to 20 mumol per kg, that of plasma increased to 0.28 mumol per 1 at the end. A strong vitamin A deficiency (liver concentration: less than 10 mumol/kg) may cause a decrease of vitamin A concentration of blood. 相似文献
37.
We derived an allometric model of the height–diameter curve for even-aged pure stands, which was a modification of the earlier
model proposed by Inoue (2000a). An individual-dependent allometric equation was used as the height–diameter curve. Using
the discriminant analysis method, all trees composed of a stand were stratified into upper and lower trees. It was assumed
that both relationships between mean tree height H
m
and upper tree height H
u
and between mean DBH D
m
and mean DBH of upper trees D
u
could be described by the time-dependent allometric power equations. The height–diameter curve showed an average relationship
between tree height and DBH of a given stand at a given time, and hence it could be assumed that the height–diameter curve
contained two points (D
m
, H
m
) and (D
u
, H
u
). With these assumptions, we derived an allometirc model of height–diameter curve, which allowed the coefficients of the
curve to be estimated from mean tree height and mean DBH. The proposed model was fitted to Japanese cedar (Cryptomeria japonica D. Don) data. The error ratio of the allometric model ranged from 2.254% to 13.412% (mean = 6.785%), which was significantly
smaller than that of the earlier model. When the error of mean tree height was ±1.0 m or less, the effect of the error of
mean tree height on the error ratio was comparatively small. This suggested that the error of ±1.0 m in mean tree height could
be accepted in the estimation of height–diameter curve using the allometric model. These features enable us to combine the
allometric model with Hirata’s vertical angle-count sampling or growth models. In conclusion, the allometric model would be
one of the most practical and convenient approaches for estimating the height–diameter relationship of even-aged pure stands. 相似文献
38.
Antti Oksanen Kjetil ?sbakk Marja Raekallio Mauri Nieminen 《Acta veterinaria Scandinavica》2014,56(1)
Background
Overwintering (breeding) reindeer (Rangifer tarandus tarandus) are commonly treated with ivermectin against parasitic infestations once yearly in autumn-winter roundups. The only preparations registered to reindeer are those for subcutaneous injection. However, also oral extra-label ivermectin administration is used. Twenty-six, 8-month-old reindeer calves were randomly allocated into three groups. Group 1 (n = 9) received oral ivermectin mixture (Ivomec® vet mixt. 0.8 mg/ml, oral ovine liquid drench formulation), Group 2 (n = 9) oral ivermectin paste (Ivomec® vet 18.7 mg/g equine paste), and Group 3 (n = 8) subcutaneous injection of ivermectin (Ivomec® 10 mg/ml vet inj.), each group at a dose of 200 μg/kg body weight. Blood samples were collected at treatment and at days 1, 2, 3, 6, 9 and 16 post treatment. Plasma concentrations of ivermectin were determined by high-pressure liquid chromatography (HPLC) with fluorescence detection.Results
The peak plasma concentration (Cmax) was reached by 2 days after each treatment. The Cmax and Area Under Curve (AUC) differed significantly between the groups: Cmax was 30.2 ± 3.9, 14.9 ± 5.7 and 63.1 ± 13.1 ng/ml, and AUC∞ was 2881 ± 462, 1299 ± 342 and 6718 ± 1620 ng*h/ml for groups 1, 2 and 3, respectively (mean ± standard deviation).Conclusions
The differences in plasma concentrations of ivermectin are concomitant with earlier observed differences in antiparasitic efficacy, which discounts the use of the equine paste in reindeer in favour of the oral ovine liquid drench formulation, or preferably, the reindeer-registered subcutaneous injection formulation. 相似文献39.
Shinpei?WadaEmail author Chutima?Hanjavanit Osamu?Kurata Kishio?Hatai 《Fisheries Science》2005,71(3):682-684
40.