Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate,suppresses FcepsilonRI expression in human basophilic KU812 cells

We previously found that the O-methylated derivative of (-)-epigallocatechin-3-O-gallate (EGCg), (-)-epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG' '3Me), has potent antiallergic activity. The high-affinity IgE receptor, FcepsilonRI, is found at high levels on basophils and mast cells and plays a key role in a series of acute and chronic human allergic reactions. To understand the mechanism of action for the antiallergic EGCG' '3Me, the effect of EGCG' '3Me on the cell surface expression of FcepsilonRI in human basophilic KU812 cells was examined. Flow cytometric analysis showed that EGCG' '3Me was able to decrease the cell surface expression of FcepsilonRI. Moreover, immunoblot analysis revealed that total cellular expression of the FcepsilonRI alpha chain decreased upon treatment with EGCG' '3Me. FcepsilonRI is a tetrameric structure comprising one alpha chain, one beta chain, and two gamma chains. The level of mRNA production of each subunit in KU812 cells was investigated. EGCG' '3Me reduced FcepsilonRI alpha and gamma mRNA levels. The cross-linkage of FcepsilonRI causes the activation of basophils, which leads to the secretion of inflammatory mediators including histamine. EGCG' '3Me treatment inhibited the FcepsilonRI cross-linking-induced histamine release. These results suggested that EGCG' '3Me can negatively regulate basophil activation through the suppression of FcepsilonRI expression.     

Geographical and Temporal Variations of Chemical Constituents in Winter Precipitation Collected in the Areas along the Coast of the Sea of Japan

The geographical and temporal variations of chemical constituents in winter precipitation collected in the areas along the coast of the Sea of Japan (AASJ) were discussed by analyzing the data obtained in the 1st and 2nd National Acid Deposition Survey by Japan Environmental Laboratories Association. In western Tohoku (WT) and Hokuriku (HR) areas in AASJ, in spite of large amounts of precipitation in winter, concentrations of non sea salt (nss-)SO₄ ^2? are not as low as the other areas, and nss-Ca²⁺ in these areas is lower than the other areas. As a result, H⁺ concentrations of precipitation in these areas are somewhat higher than other areas. From the temporal analysis of daily sampled data and back trajectory analysis of air mass, it was found that the concentrations of nss-SO₄ ^2?, NO₃ ^?, NH₄ ⁺ and nss-Ca²⁺ are correlatively varied when air mass come from the Asian Continent, showing higher concentrations at the western sites in AASJ and depending on the meteorological conditions such as the direction of in flow air mass.     

Furazolidone induces the activity of microsomal enzymes that metabolize furazolidone in chickens

The nitrofuran antibacterial agent furazolidone (FZ) is still used in veterinary medicine in some countries in the Middle and Far East. The present study aimed to show the effect of FZ on the activity of microsomal enzymes that metabolize FZ, and to identify the enzyme that contributes to FZ metabolism in chickens. Wistar rats and White Leghorn chickens were administered FZ once a day for four consecutive days. FZ metabolism was accelerated by FZ administration in chickens, but not in rats. The elevation of FZ metabolism coincided with the induction of NADPH cytochrome P450 reductase (CPR) activity in chickens, but such induction was not observed in rats. FZ metabolizing activities were inhibited in the presence of a CPR inhibitor (diphenylene iodonium chloride) but not by the addition of archetypal cytochrome P450 inhibitors (CO or n-octylamine). The preset study concluded that FZ accelerated its own metabolism in the chicken by induction of the activity of CPR.     

Preimplantation development of embryos in labrador retrievers

Preimplantation development of canine embryos is not well understood. To understand the timing of preattachment embryogenesis relative to the luteinizing hormone (LH) surge, early embryonic development was examined in Labrador Retrievers after artificial insemination. The embryos migrated from the oviduct to the uterus beginning on day 11 after the LH surge. This transport must be completed within 24 h. By day 13 after the LH surge, all of the embryos had moved and were localized in the uterus. The embryos developed to the morula stage within 11-13 days and to the blastocyst stage within 14 days after the LH surge, respectively. These findings add to the current understanding concerning the physiology of preimplantation development and should help further develop assisted reproductive techniques in canine species, such as cryopreservation and subsequent embryo transfer.     

Accuracy of imputation of single nucleotide polymorphism marker genotypes from low‐density panels in Japanese Black cattle

Using target and reference fattened steer populations, the performance of genotype imputation using lower‐density marker panels in Japanese Black cattle was evaluated. Population imputation was performed using BEAGLE software. Genotype information for approximately 40 000 single nucleotide polymorphism (SNP) markers by Illumina BovineSNP50 BeadChip was available, and imputation accuracy was assessed based on the average concordance rates of the genotypes, varying equally spaced SNP densities, and the number of individuals in the reference population. Two additional statistics were also calculated as indicators of imputation performance. The concordance rates tended to be lower for SNPs with greater minor allele frequencies, or those located near the ends of the chromosomes. Longer autosomes yielded greater imputation accuracies than shorter ones. When SNPs were selected based on linkage disequilibrium information, relative imputation accuracy was slightly improved. When 3000 and 10 000 equally spaced SNPs were used, the imputation accuracies were greater than 90% and approximately 97%, respectively. These results indicate that combining genotyping using a lower‐density SNP chip with genotype imputation based on a population of individuals genotyped using a higher‐density SNP chip is a cost‐effective and valid approach for genomic prediction.     

MARINE FISH PRODUCTION AND MARKETING FOR A CHINESE FOOD MARKET: A TRANSACTION COST PERSPECTIVE

ABSTRACT

From a transaction cost perspective, this paper shows how the tradition of consuming marine fish by the majority of Hong Kong's Chinese citizens has shaped the means and modes of marketing seafood in Hong Kong. It is argued that consumer preferences stimulate aquaculture as a non-open access measure to bypass state fish marketing regulations. This has resulted in two outcomes. First, the transaction cost savings on metering output quantity and quality under private property have led not only to the collapse of the state monopoly on marine fish marketing, but also to the emergence of the (then new but) currently popular kind of Chinese seafood restaurant in Hong Kong. Second, consumer preferences for variety have shaped the form of coordination between the producer and the consumer. The advantage of market coordination has led to the emergence of a system of wholesaling and retailing rather than vertical integration. Issues concerning the emergence of Hong Kong as a regional live marine fish production and trading centre are discussed in terms of the contribution of local mariculture to sustainable development.     

Susceptibility of Commercial Tomato Cultivars to Bacterial Wilt in Hydroponic System

Commercially available tomato cultivars were hydroponically cultured for inoculation, with Ralstonia solanacearum (K-101), which causes bacterial wilt, by pouring an inoculum suspension into the nutrient solution. Cultivar susceptibility to the bacteria was evaluated, based on the highest percentage of wilting. Because the length of time for wilt appearance varied among cultivars, some cultivars appeared to be suppressive to the translocation and/or multiplication of the invading pathogen. Thus, this hydroponic inoculation system is effective for examining levels of susceptibility in tomato cultivars to bacterial wilt. Received 13 December 2000/ Accepted in revised form 27 March 2001     

Protection from pseudorabies virus challenge in mice by a combination of purified gII, gIII and gVI antigens.

The antigens gII, gIII and gVI were purified from the lysates of the pseudorabies virus (PRV) -infected HmLu-1 cells using Sepharose 4 B coupled with MAbs against these antigens. Mice immunized with either gII, gIII, gVI antigen or a mixture of them were challenged intraperitoneally with 8.5 x 10(3) plaque forming units of PRV. All the mice immunized with 1.5 and 4.5 micrograms of the mixture and 4.5 micrograms of the gIII antigen survived. The sera of mice immunized with the mixture had virus neutralizing activity which was independent of complement, hemagglutination inhibition activity, and recognized major (93 kilodaltons) and minor (129, 74, 68 and 50 kilodaltons) PRV proteins under reducing conditions in western blotting. The serological activity levels in the lower survival group were not different from those in the complete survival. These results indicate that the mixture of each glycoprotein is more effective for eliciting of protective immunities in mice and that serological activity do not always correlate with protection.