黄瓜低温弱光耐受性机理及其应用研究的主要进展

选择低温弱光抗性水平不同的欧洲温室型、欧亚杂交型、华北温室型、华北露地型黄瓜(Cucumis sati- vus L.)的16个品种为试验材料,以光合代谢为切入点,开展苗期光合速率、光补偿点、1,5-二磷酸核酮糖羧化酶 (rubisco)活性和叶绿素a荧光动力学曲线的系统研究,结果表明:单一偏低温和偏低温弱光协效应未明显伤害光合系统,而临界低温可能伤及PSⅡ;偏低温和弱光组合时,弱光对黄瓜幼苗的影响起主导作用,弱光与临界低温组合时,临界低温起主导作用;偏低温弱光(单一弱光)下的叶面积增长量和临界低温下的冷害指数可分别作为评价黄瓜对偏低温弱光(单一弱光)耐受性和临界低温耐受性的比较稳定可靠的指标,据此设计了相关的评价指标体系。用117个重组自交系为材料,以弱光下叶面积增长量为指标进行弱光耐受性评价并以RAPD、AFLP、SSR分子图谱为基础,通过区间作图,检测到5个与黄瓜弱光耐受性有关的QTLs。采用mRNA差异显示银染技术克隆得到黄瓜冷敏型品种津研4号低温锻炼中特异表达基因的cDNA克隆(ccr18),其大小为639 bp。在基因组中以单拷贝或低拷贝形式存在。序列同源性比较表明它与拟南芥[Arabidopsis thaliana(L．)Heynh．]染色体ⅣBAC库中的 F14P3基因组序列具有88％的同源性。     

西瓜核型雄性不育两用系POD同工酶研究

用PAGE(聚丙烯酰胺凝胶)电泳技术,研究了西瓜核型雄性不育两用系不同器官的POD(过氧化物酶)同工酶酶谱差异。试验结果表明,不育株与可育株主要差异在雄花蕾,表现为不育株较可育株多2条活性强的酶带;其他器官差异小,仅表现为酶谱带强弱不同。并对POD同工酶与雄性不育性的相关性进行了研究。     

L-肉碱在水产养殖中的应用

L-肉碱是一种具有多种生理功能的新型添加剂。在水产饲料中添加适量L-肉碱不但可以提高饲料利用效率、降低饵料系数、加快水产动物的生长,还能节约蛋白质日粮,改善肉质。     

不同生长年限王草的营养价值研究

本文对生长第1、2、3年的王草在100cm高度时刈割的营养成分及其体外干物质、有机物降解率进行分析研究。结果表明:不同生长年限王草第1年的粗蛋白质显著高于第2、3年(P<0.05),而中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)显著低于第3年(P<0.05)。干物质、有机物的降解率第1年最高,第3年的最低,但差异不大。     

园林布局中必然性和偶然性要素的辩识与运用

阐释了园林构成要素中的必然性要素和偶然性要素及其相互关系与运用意义；以昆明世博园中“齐鲁园”为例，辩识了“齐鲁园”中必然性与偶然性园林要素，分析了诸要素在特定园林环境布局中的运用。     

实施GMP后我国兽药产业的竞争态势和应对策略

兽药产业实施GMP以后,根据波特五力模型分析,我国兽药企业竞争竞争态势会发生如下趋势:销售商和原材料供应商将成为兽药企业争夺的焦点。兽药产业的潜在进入者和替代产品对兽药企业威胁都不大,兽药企业面对的经营压力主要来自于业内同类企业和国外兽药企业的竞争。面对竞争态势的变化,兽药企业的应对策略主要是加强与原料药企业的合作,不断完善产品营销网络,实施专业化规模生产。     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

The application of atomic force microscope in clinic diagnosis of erythrocytes

AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589. 0 nm and a length of 646. 7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on.     

Effects of TGF-β1 and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells(HSPC)

AIM:To study the effect of TGF-β₁ and TNF-α antisense PS-ODNS on ex vivo expansion of hematopoietic stem/progenitor cells (HSPC). METHODS:CD₃₄⁺cells were purified from fresh umbilical cord blood by immunomagnetic beads, and mononuclear cells were purified from bone marrow by Ficoll-hypaque. The effects of TGF-β₁ and /or TNF-α antisense PS-ODNS on ex vivo expansion of CD₃₄⁺ cells、CFU-GEMM、CFU-GM、CFU-E and BFU-E were detected by using liquid and semi-solid culture systems.RESULTS:TGF-β₁ antisense PS-ODNS cooperated with cytokines increased the number of CD₃₄⁺ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E, which was as 4, 2.6, 2.7, 1.8, 2.1 times as that of the control (the cytokines combination), respectively. TNF-α antisense PS-ODNS cooperated with cytokines respectively increased the number of CD₃₄⁺ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 4, 2.9, 2.6, 1.7, 1.8 times as that of the control. The above two antisense PS-ODNS cooperated with cytokines could respectively increased the number of CD₃₄⁺ cells, CFU-GEMM, CFU-GM, CFU-E and BFU-E by 5.3, 2.1, 2.7, 1.9, 1.8 times as that of the control.CONCLUSION:Inhibition of endogenous TGF-β₁ and TNF-α by antisense PS-ODNS will be one of the effective methods to expand HSPC ex vivo.     

荒漠河岸林生态系统的结构分析

在以塔里木河为中心的荒漠河岸林生态系统中，植物种类及生活型单一；动物种群不够丰富，荒漠环境下的微生物种群在河岸林风沙土中，细菌，放线菌及真菌数量分别占63.1%,26.8%和10.1%，在柽柳根际土壤中，细菌及放线菌分别占96.74%和3.26%，而胡杨根际土壤中则全部为真菌，生物种群的这种特点，决定了荒漠生态系统中生态过程的特殊性，生产用水及生态用水的矛盾长期得到解决，造成了天然植被衰退，土壤干旱，盐渍化，沙漠化发展，加之大风及沙尘暴频繁，生态环境脆弱特征明显，以胡杨为建群种的荒漠生态系统的组成群落十分多样，随着系统内的水文，土壤，植被，地形及气候条件的相应更替，群落的结构及组成也自然要随之变化，最终一种生态系统将为不同群落生态系统所替代，以水资源为中心，大力营造生态工程，优化系统结构，实午动态监测与管理，实现荒漠河岸林生态系统的可持纽发展。