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201.
Haruhiko Inoue Daichi Mizuno Michiko Takahashi Hiromi Nakanishi Satoshi Mori Naoko K. Nishizawa 《Soil Science and Plant Nutrition》2013,59(7):1133-1140
We identified four putative AtFRD3-like genes (OsFRDL) in the rice genome that exhibited 39.1 to 56.7% amino acid sequence similarities to Arabidopsis FRD3. Of these, we cloned three OsFRDL genes from a cDNA library prepared from iron-deficient rice roots: OsFRDL1, OsFRDL2, and OsFRDL3. OsFRDL1 was expressed weakly in Fe-sufficient roots, and slight expression was induced in the roots of Fe-deficient plants. OsFRDL2 was expressed constitutively in both roots and leaves, and Fe deficiency reduced its expression in leaves. OsFRDL3 was expressed in leaves, but not in roots; Fe deficiency induced slight expression in leaves. An OsFRDL1-sGFP fusion protein was localized in the plasma membrane in onion epidermal cells. The promoter GUS analysis showed that OsFRDL1 was localized in the cells involved in long-distance transport, in both Fe-sufficient and Fe-deficient plants. Furthermore, OsFRDL1 expression was observed during the reproductive stage. These results suggest that OsFRDL1 is a transporter that resides in the plasma membrane of cells involved in long-distant transport. 相似文献
202.
Yoko Tanaka Akari Fukuda Seiji Nagai Toru Fujiwara Yoshihito Suzuki Isomaro Yamaguchi 《Soil Science and Plant Nutrition》2013,59(8):1281-1285
GA1 and GA4 are active gibberellis (GAs) in rice plants, which are synthesized from GA19 and GA24, respectively. However, the plant tissues where active GAs biosynthesis occurs and the route of their transport have not been fully elucidated. To study the importance of vascular tissues for the function of GAs, cDNA encoding anti-GA24/19 single-chain variable fragment (scFv) was expressed under the control of the thioredoxin h promoter, which is active in companion cells of phloem tissues. Thetransgenic rice plants showed a dwarf phenotype with increased levels of GA19. This indicated that the expression of the anti-GA24/19 antibody in companion cells changed the synthesis and/or translocation of GAs in rice plants effectively, suggesting that the vascular tissues play an important role in the function of GAs in rice plants. 相似文献
203.
Hiromi Kato Yasuhito Igarashi Yukiko Dokiya Yoko Katayama 《Water, air, and soil pollution》2012,223(1):159-167
Atmospheric carbonyl sulfide (COS) mixing ratios measured over 24 h during five summer campaigns (2003–2007) in a forest at
the foot of Mt. Fuji, Japan (35°21′ N, 138°43′ E; 1,300 m above sea level, a.s.l.) and at the summit (3,776 m a.s.l.) were
compared. COS levels were lower at the foot than at the summit during four out of five summer campaigns. The ratios of COS
mixing ratios at the foot of Mt. Fuji to those at the summit ranged from 0.7 to 0.9. These results provide evidence of biological
consumption of COS in the East Asian atmospheric boundary layer. We also measured the vertical profile of ambient COS below
the forest canopy. These data showed a clear gradient of COS mixing ratio: in the lowermost 1 m of the boundary layer, COS
mixing ratios decreased markedly downward. Two of the different kinds of vertical distribution of COS presented here support
the role of soil as a sink of atmospheric COS described by previous research using dynamic enclosure experiments. 相似文献
204.
Nahoko IEDA Yoshihisa UENOYAMA Yoko TAJIMA Tomoko NAKATA Masatoshi KANO Yousuke NANIWA Youki WATANABE Shiori MINABE Junko TOMIKAWA Naoko INOUE Fuko MATSUDA Satoshi OHKURA Kei-ichiro MAEDA Hiroko TSUKAMURA 《The Journal of reproduction and development》2014,60(4):312-316
Puberty is associated with an increase in gonadotropin secretion as a result of an increase in gonadotropin-releasing hormone
(GnRH) secretion. Kisspeptin is considered to play a key role in puberty onset in many mammalian species, including rodents,
ruminants and primates. The present study aimed to determine if changes in hypothalamic expression of the KISS1
gene, encoding kisspeptin, are associated with the onset of puberty in pigs. The animals (n=4 in each group) were perfused with 4%
paraformaldehyde at 0, 1, 2, 3 and 4 months old, as prepubertal stages, and at 5 months old, as the peripubertal stage, following
each blood sampling. KISS1 gene expressions in coronal sections of brains were visualized by in
situ hybridization. Plasma luteinizing hormone (LH) was measured by radioimmunoassay. KISS1 mRNA
signals were observed in the arcuate nucleus (ARC) at all ages examined without any significant difference in the number of
KISS1-expressing cells, indicating that the KISS1 gene is constantly expressed in the ARC
throughout pubertal development in pigs. The plasma LH concentration was the highest in 0-month-old piglets and significantly
decreased in the 1- and 2 month-old groups (P<0.05), suggesting a developing negative feedback mechanism affecting gonadotropin
release during the prepubertal period. Considering the potent stimulating effect of kisspeptin on gonadotropin release in
prepubertal pigs, kisspeptin secretion rather than kisspeptin synthesis may be responsible for the onset of puberty in pigs. 相似文献
205.
Hiroyuki Kurokochi Hiroshi Uchiyama Mikio Hasegawa Yoko Saito Yuji Ide 《Journal of Forest Research》2014,19(5):469-472
Ailanthus altissima is a woody plant that has become invasively naturalized in many countries. Using nuclear SSR markers, we studied specimens of this species collected throughout Japan and found an individual (labeled Aa-1) that was not diploid. We examined pollen morphology and ploidy level of Aa-1 by optical microscopy and flow cytometry (FCM), respectively. Some pollen grains were morphologically abnormal and much larger than normal. FCM analysis demonstrated that Aa-1 was triploid. Formation of abnormal pollen indicates that Aa-1 may well have reduced fertility compared to normal diploids. If this were the case, vegetative progeny Aa-1 could be used as ornamentals without attendant risks of future naturalization. 相似文献
206.
Shouya Naruse Mayuko Ogino Takao Nakagawa Yoko Yasuno Akiya Jouraku Takahiro Shiotsuki Tetsuro Shinada Ken Miura Chieka Minakuchi 《Journal of Pesticide Science》2021,46(1):60
Insect juvenile hormone (JH) mimics (JHMs) are known to have ovicidal effects if applied to adult females or eggs. Here, we examined the effects of exogenous JHMs on embryonic development of the bean bug, Riptortus pedestris. The expression profiles of JH early response genes and JH biosynthetic enzymes indicated that JH titer was low for the first 3 days of the egg stage and increased thereafter. Application of JH III skipped bisepoxide (JHSB3) or JHM on Day 0 eggs when JH titer was low caused reduced hatchability, and the embryos mainly arrested in mid- or late embryonic stage. Application of JHMs on Day 5 eggs also resulted in an arrest, but this was less effective compared with Day 0 treatment. Interestingly, ovicidal activity of synthetic JHMs was much lower than that of JHSB3. This study will contribute to developing novel insecticides that are selective among insect species. 相似文献
207.
Yumi Umeda Tatsuya Kasai Misae Saito Hitomi Kondo Tadao Toya Shigetoshi Aiso Hirokazu Okuda Tomoshi Nishizawa Shoji Fukushima 《Journal of toxicologic pathology》2013,26(2):131-140
To evaluate pulmonary toxicity of multi-walled carbon nanotubes (MWCNTs), F344 rats of
both sexes were exposed by inhalation to 0.2, 1 or 5 mg/m3 MWCNT aerosol for 6
h/day, 5 days/week for 2 weeks using a whole-body exposure system. At the end of the
2-week exposure period, one-half of the rats were necropsied, and at the end of an
additional 4-week postexposure period, the remaining rats were necropsied. MWCNTs were
deposited in the lungs of all MWCNT-exposed groups and mostly remained in the lungs
throughout the 4-week postexposure period. Granulomatous changes in the lung were found in
the rats exposed to 5 mg/m3 MWCNTs, and these changes were slightly aggravated
at the end of the 4-week postexposure period. In the bronchoalveolar lavage fluid (BALF),
the numbers of neutrophils, percentages of bi- and multinucleated alveolar macrophages,
levels of ALP activity and concentrations of total protein and albumin were elevated in
the rats exposed to 1 and 5 mg/m3 MWCNTs. At the end of the 4-week postexposure
period, the values of the BALF parameters tended to remain elevated. In addition, goblet
cell hyperplasias in the nasal cavity and nasopharynx were observed in the rats exposed to
1 and 5 mg/m3 MWCNTs, but these lesions had largely regressed by the end of the
postexposure period. Based on the histopathological and inflammatory changes, the
no-observed-adverse-effect level (NOAEL) for inhalation of MWCNTs for 2 weeks was 0.2
mg/m3. 相似文献
208.
209.
Itou T Yoshida Y Shoji Y Sugisawa H Endo T Sakai T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(12):1351-1354
The bottlenose dolphin interleukin (IL)-8 cDNA was molecularly cloned. The dolphin IL-8 has an open reading frame of 303-bp encoding 101 amino acids. The homology of the amino acid sequence with that of other species was: sheep, 89.1%; cattle, 88.1%; pig, 85.1%; dog, 85.1%; horse, 79.2%; human, 74.5%; and macaque, 72.3%. The amino acid sequence suggested that dolphin IL-8 was a CXC chemokine. The recombinant dolphin IL-8 protein was recognized with anti-ovine IL-8 monoclonal antibody. 相似文献
210.
Michimae Y Morita T Sunagawa Y Sawada M Okamoto Y Shimada A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1155-1158
An 8-year and 6-month-old female Maltese dog showed a stoop with rigidity of her cervix and back. Neurologic examination showed loss of proprioception, and deficiency of pain response. Postmortem examination revealed the neoplastic mass replacing the central area in the cervical spinal cord at the level from 4th to 5th segments. Histologically, the mass was composed of neoplastic ependymal cells. The neoplastic cells showed marked atypism, and occasionally formed ependymal rosettes. Based on the morphologic features, the tumor was diagnosed as anaplastic ependymoma. Immunohistochemistry showed that the neoplastic cells were negative for glial fibrillary acid protein, and slightly positive for vimentin and cytokeratin. 相似文献