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161.
Yuyama T Yusa S Yoshizumi K Yamano S Murata S Hirose T Osanai R Onishi Y Osato S Sasaki C Sasaki Y Kakuda T Tsubaki S Takai S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(8):715-718
The prevalence of virulent R. equi having 15- to 17-kDa antigens (VapA) in fecal isolates from 13 thoroughbred foals and their dams on 5 farms in Kagoshima, Japan, and the plasmid profiles of VapA-positive isolates by restriction fragment digestion patterns were investigated to compare the genotypic variation among virulence plasmids of R. equi isolates from Japan. In total, 218 (24.6%) of 886 isolates from the feces of the 13 foals and 13 (12.5%) of 104 isolates from the feces of their dams demonstrated VapA-positive R. equi. Plasmid DNA preparations of 231 virulent isolates from foals and dams were analyzed by restriction enzyme digestion with endonucleases EcoRI, EcoT22I and HindIII and were divided into 3 types: 172 isolates contained a 90-kb type I plasmid, 57 contained a 90-kb type III plasmid and 2 contained a 90-kb type IV plasmid. This study demonstrates a geographic character in the distribution of virulence plasmids found in VapA-positive isolates from thoroughbred foals in Kagoshima. 相似文献
162.
Hirashima A Eiraku T Shigeta Y Kuwano E Taniguchi E Eto M 《Pest management science》2002,58(11):1118-1125
Some octopamine (OA) agonists were found to suppress the calling behaviour and pheromone biosynthesis in vitro of the Indian meal moth, Plodia interpunctella (Hübner), a stored-product pest. Compounds were screened using a calling behaviour bioassay of female P interpunctella. Three active derivatives, with activity at the nanomolar level, were identified. In order of decreasing pheromonostatic activity these were: 2-(2-ethyl-6-methylanilino)oxazolidine > 2-(2,6-diethylanilino)thiazolidine > 2-(2,6-diethylanilino)oxazolidine. These compounds showed also in vitro inhibitory activities in de novo pheromone biosynthesis. Three-dimensional pharmacophore hypotheses were built from a set of 19 compounds. Among the ten common-featured models generated by the program Catalyst/HipHop, a hypothesis including a ring aromatic group (RA), a positive ionizable group (PI) and two hydrophobic aliphatic (HpA1) features was considered to be essential for inhibitory activity in the calling behaviour and pheromone biosynthesis in vitro. Active compounds mapped well onto all the RA, PI and HpA1 features of the hypothesis. Less-active compounds were shown not to achieve the energetically favourable conformation which was found in the active molecules in order to fit the 3-D common-feature pharmacophore models. The present studies demonstrate that inhibition of calling behaviour and PBAN-stimulated incorporation of radioactivity is by OA-agonistic activity. 相似文献
163.
Cell wall directions of tracheids in transverse sections of Agathis sp. and Cryptomeria japonica and fiber bodies in Magnolia obovata were quantified by Fourier transform image analysis (FTIA). Three kinds of information about cell shapes were determined from the angular distribution functions of the net map-power spectrum pattern (NM-PSP): (1) the peak angle expresses the most frequent cell wall direction, (2) the area of the peak is indicative of cell wall length, and (3) the dispersion of the peak indicates the fluctuation of cell wall orientation. Fluctuations were expressed as the standard deviation (σ) by the use of statistical analysis. Cell wall directions in Agathis, that is, directions of the tangential and radial walls (three orientations were detected) were 0° (σ = 7°), 59° (12°), 91° (9°), and 122° (12°), respectively. Pentagonal and hexagonal models were reconstructed as cell shape models in a calculated ratio of 60 : 40. On the other hand, directions were 0° (5°), 76° (12°), 89° (5°), and 102° (12°) in a calculated ratio of 45 : 55 in Cryptomeria. Fiber bodies were extracted in Magnolia and their wall directions were 0° (5°), 50° (7°), 90° (6°), and 131° (7°), respectively. The ordinary accumulated image analysis (OAIA) was checked at the same time in Agathis, and indicated that FTIA could be used as a method of reconstruction of cell shape. 相似文献
164.
The potential of termite (Coptotermes formosanus Shiraki) and fungal attacks [Fomitopsis palustris (Berkeley et Curtis) Murrill (FFPRI 0507) and Trametes versicolor (L. ex Fr.) Quel (FFPRI 1030)] against moso bamboo (Phyllostachys pubescens Mazel) were evaluated with regard to the seasonal and height-dependent changes of the free glucose and starch contents, which
were measured by a newly developed method. The free glucose contents were generally lower in autumn and winter than in spring
and summer, whereas the lowest starch contents were obtained in August, and the contents increased almost linearly up to February
and March. In terms of the height-dependent fluctuation, the free glucose contents tended to decrease as the sampling heights
increased. There was no special correlation between the free glucose or starch contents and the consumption by termites, even
though higher mortalities were obtained in the bamboo-fed termites than in the wood-fed termites. However, a positive correlation
between the free glucose contents and mass losses of the samples at 4-m and 8-m heights from the bottom by the decay fungi
was observed. For starch, no influence on fungal attack was found. 相似文献
165.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):487-491
Case 1 involved a 4-month-old intact male Somali cat in which peripheral pulmonary artery
stenosis (PPS) was recognized after a cardiac murmur remained following patent ductus
arteriosus ligation. Case 2, which involved a 1-year-old neutered male Norwegian Forest
cat, and Case 3, which involved a 6-month-old intact female American Curl cat, were
referred, because of cardiac murmurs. Grades III to IV/VI systolic heart murmurs were
auscultated at the left heart base in all 3 cats. All cases showed bilateral pulmonary
artery stenosis, although there were no associated clinical signs. In Cases 1 and 2, the
pressure gradient through the stenosis decreased after treatment with atenolol. 相似文献
166.
Takuma AOKI Hiroshi SUNAHARA Keisuke SUGIMOTO Tetsuro ITO Eiichi KANAI Yoko FUJII 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):331-336
Infective endocarditis (IE) in dogs with cardiac shunts has not been reported previously.
However, we encountered a dog with concurrent patent ductus arteriosus (PDA) and IE. The
dog was a 1-year-old, 13.9-kg female Border collie and presented with anorexia, weight
loss, pyrexia (40.4°C) and lameness. A continuous murmur with maximal intensity over the
left heart base (Levine 5/6) was detected on auscultation. Echocardiography revealed a PDA
and severe aortic stenosis (AS) caused by aortic-valve vegetative lesions.
Corynebacterium spp. and Bacillus subtilis were
isolated from blood cultures. The dog responded to aggressive antibiotic therapy, and the
PDA was subsequently surgically corrected. After a series of treatments, the dog showed
long-term improvement in clinical status. 相似文献
167.
Takuma Aoki Hiroo Madarame Keisuke Sugimoto Hiroshi Sunahara Yoko Fujii Eiichi Kanai Tetsuro Ito 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(7):745-748
We report the case of a 4-year-old, castrated 4.2-kg Scottish fold cat with recurrent epistaxis that was unresponsive to medical therapy. Diathermocoagulation of the nasal mucosa with a diode laser controlled the epistaxis and there was no significant recurrence of epistaxis during 1 year of follow-up. 相似文献
168.
Nakaichi M Takeshita Y Okuda M Nakamoto Y Itamoto K Une S Sasaki N Kadosawa T Takahashi T Taura Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):111-115
Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors. 相似文献
169.
Overexpression of the Barley Nicotianamine Synthase Gene HvNAS1 Increases Iron and Zinc Concentrations in Rice Grains 总被引:1,自引:0,他引:1
Hiroshi Masuda Kanako Usuda Takanori Kobayashi Yasuhiro Ishimaru Yusuke Kakei Michiko Takahashi Kyoko Higuchi Hiromi Nakanishi Satoshi Mori Naoko K. Nishizawa 《Rice》2009,2(4):155-166
In humans, iron (Fe) and zinc (Zn) deficiencies result in major worldwide health problems. Transgenic technologies to produce Fe- and Zn-biofortified rice varieties offer a promising potential solution. Nicotianamine, the precursor of phytosiderophores, chelates Fe2+ and Zn2+ and plays an important role in transporting these metals to both vegetative and reproductive organs within the plant. Our objective was to increase Fe and Zn contents in rice grains by overexpressing the barley nicotianamine synthase gene HvNAS1. HvNAS1-overexpressing transgenic rice showed increased HvNAS1 expression and subsequent increases in endogenous nicotianamine and phytosiderophore content in shoots, roots, and seeds. Fe and Zn concentrations in polished T1 seeds from transgenic plants increased more than three and twofold, respectively; Fe and Zn concentrations also increased in both polished and brown T2 seeds. These results suggest that the overproduction of nicotianamine enhances the translocation of Fe and Zn into rice grains. 相似文献
170.
Formation of grain chalkiness and changes in water distribution in developing rice caryopses grown under high-temperature stress 总被引:1,自引:0,他引:1