德援项目森林可持续经营示范区建设成效调查

为了全面掌握德援项目森林可持续经营示范区建设完成情况,评价项目实施以来取得的成效,采用实地抽样调查方法对湖北省2005年以来实施的森林可持续经营示范区建设项目进行了调查。结果表明:林分结构得到明显改善,形成了由乡土树种组成的多树种混交结构和异龄林结构的近自然森林;森林植物种类明显增多,形成比实施前多5种以上的植物;森林质量显著提高,林木平均胸径增长50%以上,平均树高增长10%以上,平均每公顷蓄积增长50%以上;森林郁闭度保持在0.8~0.9,林地土壤腐殖质层厚达到10cm左右。示范区的示范效应突显,为项目区全面开展森林可持续经营提供了技术保障和科学依据。     

不同监测方法对土壤侵蚀监测结果的影响——以江西省潋水河流域为例

分析土壤侵蚀监测的尺度大小问题,认为在南方红壤区,区域尺度面积范围在500km^2以上较为合理,属于土壤侵蚀宏观监测范畴。将人机交互目视解译法、RUSLE模型法和流域卡口站观测法3种方法应用于江西省兴国县潋水河中尺度流域内,进行土壤侵蚀监测及对比。结果表明：用人机交互目视解译法监测的2000年土壤侵蚀量最大,约是流域卡口站观测结果的6．2倍,约是模型法监测结果的1．9倍,差异很大。其主要原因是流域卡口站观测法只观测了悬移质输沙量,推移质输沙量无法观测。此外,利用模型法,不同比例尺DEM对监测结果影响很大,其差异主要体现在土壤侵蚀强度为轻度和微度级别上。在分析不同监测方法对土壤侵蚀监测结果差异的基础上,对我国今后土壤侵蚀宏观监测工作提出了建议。     

Stability evaluation of selected polyphenols and triterpene glycosides in black cohosh

Black cohosh ( Actaea racemosa L., syn. Cimicifuga racemosa L.) is rich in both triterpene glycosides and polyphenols, which have various biological activities that may be important to its medical use. To evaluate the stability of the polyphenolic constituents and triterpene glycosides of black cohosh, experiments were conducted using three sample types: plant material, extracts of black cohosh, and encapsulated commercial extract. The samples were stored at various temperatures and humidity conditions. Three triterpene glycosides and six major polyphenols in black cohosh were quantitatively measured with an HPLC-PDA method at 0, 3, 6, and 9 weeks. The triterpene glycosides were stable at the tested conditions, whereas the polyphenols were stable only at room temperature and low humidity and not stable at higher temperature and/or humidity due to hydrolysis and/or oxidation. The rate of compound decomposition depended upon the chemical structure of the individual polyphenols. Polyphenols in the extracts decomposed more readily than those in plant material.     

Effects of dietary rosemary extract supplementation on growth performance,nutrient digestibility,antioxidant capacity,intestinal morphology,and microbiota of weaning pigs

Rosemary (Rosmarinus officinalis L.) extract (RE) has multiple pharmacological and biological activities, including the use as a food additive and medicine. This study was conducted to investigate the effects of dietary RE supplementation on the growth performance, nutrient digestibility, antioxidant capacity, intestinal morphology, and microbiota of weaning piglets. A total of 192 crossbred weaned piglets [Duroc × (Large White × Landrace)] (initial body weight = 6.65 ± 0.33 kg, weaned days = 23 ± 1 d) were group housed (six pigs per pen; n = 8 pens/treatment). Pigs were fed a corn–soybean meal-based control diet or the basal diet supplemented with 100, 200, or 400 mg/kg RE. Pigs were allowed ad libitum access to fed for 21 d. The growth performance and apparent total tract digestibility of nutrients, and intestinal morphology and antioxidant status were evaluated. The components of the microbial microflora were also determined in the cecal samples. Compared with the control, dietary supplementation with RE increased the final body weight, average daily gain, and average daily feed intake (linear, P = 0.038, 0.016, and 0.009, respectively), and decreased the diarrhea ratio in piglets (linear, P < 0.05). The digestibility of crude protein (linear, P = 0.034) and gross energy (linear, P = 0.046) increased with treatment with RE. Piglets fed RE showed longer villus height (linear, P = 0.037 and 0.028, respectively) and villus height/crypt depth (linear, P = 0.004 and 0.012; quadratic, P = 0.023 and 0.036, respectively) in the jejunum and ileum, in addition to a lesser crypt depth in the jejunum (linear, P = 0.019) and ileum (quadratic, P = 0.042). The addition of RE increased the activity of superoxide dismutase (linear, P = 0.035 and 0.008, respectively) and glutathione peroxidase activity (linear, P = 0.027 and 0.039, respectively) and decreased the content of malondialdehyde (linear, P = 0.041 and 0.013; quadratic, P = 0.023 and 0.005, respectively) in the serum and liver. Dietary RE supplementation, compared with the control, increased the number of Bifidobacterium (linear, P = 0.034) and Bacteroidetes (linear, P = 0.029), while decreased Escherichia coli (linear, P = 0.008; quadratic, P = 0.014) in the cecal contents. Thus, dietary RE supplementation can improve growth performance, nutrient digestibility, antioxidant capacity, intestinal morphology, and the microbiota in weaned piglets, and 200 mg/kg may be considered the optimum dosage.     

采用显微图像识别技术快速检测食品细菌总数

提出利用显微图像技术与BP神经网络技术检测食品细菌总数的方法,以适应快速准确检测的要求.设计了一套基于计算机视觉技术的食品细菌总数检测系统.该系统运用显微图像技术和BP神经网络技术对试验样本的细菌总数进行分析和计数.该系统由Visual C 6.0平台开发,对细菌图像的分析快速、准确,一帧图像可采集的细菌数量小于500个,对1组图像(10帧图像)的分析时间不大于30 s.比较平板计数法,该方法在牛奶、果汁和牛肉的细菌总数检测试验中,两种方法结果无显著差异(t检验结果p＞0.05).     

Isolation and characterisation of phages against Pseudomonas syringae pv. actinidiae

Purpose: Pseudomonas syringae pv. actinidiae causes bacterial canker of kiwifruit and is responsible for severe economic losses and emergence of drug-resistant bacteria. Bacteriophages are viruses that infect target bacterial hosts and may be the best strategy to prevent and control kiwifruit canker disease. The objective of this experiment was to monitor the prevalence of Pseudomonas syringae pv. actinidiae and provide insight for the use of phages in biological control.

Materials and methods: In this study, 52 strains of Pseudomonas syringae pv. actinidiae were isolated from 68 stem samples of kiwi plant (cv. Hongyang & Jinkui). Following polymerase chain reaction (PCR) analysis, 15 isolates belonging to biovar 3 were identified, one of which was named XWY0007 and used as the target strain to isolate the phages. Thirty-six phages were isolated and purified from a total of 51 surface water samples collected in Shanghai. All phages were identified by transmission electron microscopy (TEM) and their host ranges were evaluated. Three phages, designated φXWY0013, φXWY0014 and φXWY0026 were selected and further characterised using one-step growth curve and stability at different temperatures and pH.

Results and conclusions: The isolated phages are promising for use as antimicrobials against bacterial canker in kiwi. This report is regarding Pseudomonas syringae pv. actinidiae and its phages from major areas of kiwifruit cultivation.     

Differential structure and physiological response to canker challenge between ‘Meiwa’ kumquat and ‘Newhall’ navel orange with contrasting resistance

Asiatic citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is one of the most detrimental diseases affecting citrus production. ‘Meiwa’ kumquat (Fortunella crassifolia) and ‘Newhall’ navel orange (Citrus sinensis) are two genotypes with contrasting Xcc resistance. In the current study, efforts were made to perform pairwise comparison between them in terms of structural difference and biochemical response to Xcc attack. Pinprick inoculation demonstrated that ‘Meiwa’ had lower disease incidence rate and smaller lesion size, concurrent with significantly less bacterial number at the inoculation sites relative to ‘Newhall’. Microscopic observation demonstrated that stomatal density and size of ‘Meiwa’ were significantly smaller than ‘Newhall’. Stomatal apertures of ‘Meiwa’ were smaller than ‘Newhall’ measured at 2 h after their leaves were immersed in Xcc suspension, consistent with the bacteria number in the internal tissues counted at 4 d. Epicuticular wax content of ‘Meiwa’ was significantly higher than that of ‘Newhall’, while no difference in palisade and spongy tissue was observed between them. Enzymatic activity assay showed that after Xcc inoculation, ‘Meiwa’ exhibited significantly higher activities of catalase (CAT), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) in comparison with ‘Newhall’ at most of the sampling time points. Our data showed that the two genotypes with contrasting Xcc resistance exhibited clear difference in the structure and physiological response, which may provide information to facilitate our understanding of Xcc resistance.     

Response of N2O emission to straw retention under two contrasting paddy water conditions: a field experiment over rice–rice-fallow rotation

Paddy and Water Environment - Few studies are available on comprehensive impacts of straw retention and water regimes on nitrous oxide (N2O) emission from rice–rice-fallow rotation systems. A...     

浅析我国南方冬闲田种植一年生黑麦草的应用研究

针对我国南方地区冬闲田数量多、利用率低的现象,充分利用冬闲田种草养畜,不仅可以解决牲畜季节性青绿粗饲料缺乏问题,还能促进农民增收和农业可持续发展。一年生黑麦草由于适生范围广,鲜草产量高,营养丰富且柔嫩多汁,适口性好,家畜家禽喜欢采食,深受农户青睐,成为一种极具发展前途的越年生优质牧草,近年来在畜牧业生产中得到广泛应用。本文从一年生黑麦草生物性状、营养特性、冬闲田的丰产栽培技术、青贮技术以及在各类畜禽的饲喂研究角度进行综述,并指出今后的研究重点和方向。旨在为一年生黑麦草在畜牧业中的进一步推广应用提供参考。