Association between weight change during initial chemotherapy and clinical outcome in dogs with multicentric lymphoma

The majority of the known prognostic factors in dogs with lymphoma have been evaluated before treatment commences or at the time of diagnosis. Prognostic factors evaluated during the initial phase of treatment are less described but may provide important clinical information. In this retrospective study, 82 canine lymphoma patients were categorized according to the weight change between diagnosis and after 5 weeks of chemotherapy. Dogs that gained greater than 5% or lost greater than 5% of initial body weight were categorized as increased‐ or decreased‐weight groups, respectively. Those in which weight changed less than 5% were categorized as the maintained‐weight group. The median progression‐free survival (PFS) in the increased‐weight group, maintained‐weight group and decreased‐weight group was 226, 256 and 129 days, respectively. The decreased‐weight group had significantly shorter PFS than the increased and maintained groups (P = .023, P = .003, respectively). The median survival time (ST) in the increased‐weight group, maintained‐weight group and decreased‐weight group was 320, 339 and 222 days, respectively. There was no significant difference in ST among the three groups (P = .128). In Cox‐regression results, weight change group and initial body weight were significant risk factors associated to PFS (P = .007, P = .001, respectively) while only patient's initial body weight was a significant risk factor to ST (P = .013). In conclusion, evaluation of initial body weight and weight changes over time can provide valuable information regarding PFS and ST in dogs with multicentric lymphoma.     

抗旱、耐盐碱、矮秆陆地棉数量性状的遗传相关及主成份分析

对从国内外引进的抗旱、耐盐碱、矮杆棉花资源材料 ,经抗旱、耐盐碱、矮杆性鉴定和比较 ,选取了其中表现较好的 1 9份材料 ,进行了遗传相关和主成份分析 ,结果表明 :在 5 0 mmol· L- 1Na Cland1 0 0 mmol· L- 1Na Cl胁迫下的出苗率间呈正相关 ,抗盐性与产量间呈负相关 ;第一棉铃高度与纤维品质性状呈正相关。抗旱、耐盐碱、矮杆棉花材料的前 6个因子贡献率达 86.5 % ,其分别为第一棉铃高度因子、果枝数因子、单株结铃数因子、矮杆因子、单铃重因子等     

插秧机作业面积测量远程监测系统软件设计——基于VisualBasic.NET 2010

为实现对插秧机作业区域自动识别和作业面积的自动测量,实时掌握跨区作业插秧机远程作业面积参量值,以VisualStudio平台上的VisualBasic.NET2010为开发环境,开发了一套高速插秧机跨区作业面积远程测量监测系统软件。该软件功能主要包括GPS定位轨迹及作业区域的识别、面积测量、数据通讯、数据显示和数据存贮。系统采用VisualBasic.NET2010语言进行开发,将远程跨区作业的插秧机面积测量监测数据经由GPRS网络进行无线传输到上位机,并采用SOCKET组件和ADO.NET技术实现系统数据通信,完成PC机与下位机之间数据的传输,对测量结果进行显示存储,同时通过系统误差分析完成系统的可行性判断。     

Inhibition of porcine circovirus type 1 and type 2 production in PK-15 cells by small interfering RNAs targeting the Rep gene

Porcine circovirus type 1 (PCV1) and type 2 (PCV2) are two genotypes of porcine circovirus. Both of them are presumed to be widespread in the swine population. Currently, there is no specific treatment for their infections. RNA interference (RNAi) is a sequence-specific RNA degradation mechanism mediated by small interfering RNA (siRNA), which represents a possible therapeutic application for the treatment of viral infections. In this study, three siRNA expression plasmids (pS-RepA, pS-RepB and pS-RepC) were generated to target three different coding regions of the Rep protein (Rep) of PCV. These siRNAs were used to inhibit PCV production in a porcine kidney cell line, PK-15 cells. Our results revealed that Rep gene expression was inhibited by pS-RepA, pS-RepB and pS-RepC to different degrees. Moreover, our study also showed that the production of PCV1 and PCV2 was reduced by these siRNAs. pS-RepC, which targets the middle region of Rep gene, proved to be the most efficient siRNA for inhibition of Rep expression and viral production. Taken together, our data suggest that RNAi could be investigated as a potential treatment for PCV infection.     

Characterization,phylogenetic analyses and pathogenicity of Bipolaris setariae associated with brown stripe disease on sugarcane in Yunnan,China

Brown stripe disease is a severe foliar fungal disease of sugarcane worldwide and is widespread in all sugarcane planting areas in China. Brown stripe is a major disease that seriously affects the output and quality of the sugarcane industry in Yunnan Province, China's second-largest sugar base, while the pathogen of this disease remains not yet fully understood. To address this, we isolated and identified the fungi associated with 68 leaf samples showing typical symptoms of brown stripe from 22 sugarcane varieties in different areas of Yunnan Province. A total of 113 isolates were obtained, which were morphologically similar. Of these, 64 representative isolates were sequenced for the internal transcribed spacer region (ITS), GAPDH and EF-1α loci. All representative isolates grouped with the type strain of Bipolaris setariae in the phylogenetic trees inferred with individual and concatenated sequences of ITS, GAPDH and EF1-α. Pathogenicity test results showed that B. setariae strains were able to induce typical symptoms of brown stripe. The results obtained in this study clarify that only B. setariae is associated with sugarcane brown stripe in Yunnan, China. It is recorded here for the first time as a pathogen causing sugarcane brown stripe in Yunnan, and it is able to infect many major cultivars and new varieties, posing a new threat to the sugar industry in Yunnan Province. In addition, these results provide the scientific basis for the future breeding of disease-resistant varieties and effective prevention and control of sugarcane brown stripe disease.     

丛枝菌根真菌接种对白车轴草耐盐性的影响

丛枝菌根真菌(AMF)可与植物共生形成复杂的菌丝网络,影响植物生长及抗逆能力。目前关于AMF对白车轴草耐盐性的影响尚存争议,本研究采用盆栽试验,研究在盐胁迫条件下(NaCl浓度为150 mmol·L^-1),接种AMF对拉丁诺白车轴草耐盐性的影响。结果表明,在盐胁迫条件下,与对照相比,白车轴草的生长与生理指标均受到抑制。盐胁迫下接种AMF后,白车轴草株高、干重、PSⅡ最大光能转换效率和相对含水量均有增加,丙二醛(MDA)含量以及相对电导率有所降低,渗透调节物质均有提高,其中可溶性糖(SS)和游离脯氨酸(Pro)含量分别提高了32.03%和9.42%。说明盐胁迫抑制白车轴草的生长,接种AMF增强了白车轴草抗逆适应能力,促进白车轴草的生长,增加渗透调节物质含量,提高白车轴草耐盐胁迫的能力。     

种子生产经营中存在的主要问题与对策

1存在的主要问题 1．1种子企业基础条件差《种子法》实施后，主要农作物商品种子生产、经营实行分级审批许可制度。由于《农作物种子生产经营许可证管理办法》仅对几个大方面作出规定．而具体细节上没有统一的标准。2001年以来存在着核证标准低．办证不规范．特别是核发注册资金100万元的种子企业，不同程度地存在仓储、检验室、加工车间等基础条件差或无，     

云南高原粳稻抗白叶枯病新品系云资抗21号的选育

以高产优质的云南高原粳稻品种滇系4号为轮回亲本，以IRBB21为白叶枯病抗性基因Xa21的供体亲本，通过杂交和3次回交以及5次自交形成BC3F，代材料，并结合后代材料的白叶枯病抗性鉴定及农艺性状的评价选择，选育出高原粳稻新品系云资抗21号，对云资抗21号的21个单株采用与Xa21连锁标记RM21和RM229、以及22个独立分离的SSR标记进行检测，结果表明这21个单株在RM21位点上均为IRBB21的纯合基因型，在RM229位点上有1个单株显示为轮回亲本的纯合基因型，其余的20个单株均为IRBB21的纯合基因型，而在其余22个SSR标记位点上均显示为轮回亲本的纯合基因型。该新品系白叶枯病抗性与IRBB21相近，农艺性状与轮回亲本相似，具有很好的应用前景。     

Temporal-spatial Filtering in the Preprocessing of Clinical EEG Signals

EEG spot-recording for clinical patients in the bed is always advocated due to easing patients and making them comfortable. But EEG signals recorded in the ward exposed to various noisesand interference is less effective in the EEG analysis. Atemporal-spatial filter is designed for noise removing of clinical EEG signals, the first filtering is temporal filtering using band-pass filter, and the second filtering is spatial filtering with ICA-based spatial filter. Experimental results indicate that various noises and interferences, such as power interference, blinking, eyes movements, muscle moments, ECG artifacts, etc., are removed effectively, individually or simultaneously.