Genetic mapping of a new flowering time gene on chromosome 3B of wheat

The single chromosome substitution lines of chromosome 3B of the Czech alternative wheat variety Česká Přesívka (CP 3B) into two spring varieties Zlatka and Sandra, revealed clear differences in flowering time compared to the recipient varieties. To map this gene(s), recombinant substitution lines for chromosome 3B were produced from crosses of the substitution lines with their recipient parents and genetic maps developed using SSR markers. Two populations were mapped, Sandra//Sandra 3B/Sandra (CP 3B) and Zlatka//Zlatka/Zlatka (CP 3B). Combining the genotype data with phenotype data on flowering time in five independent experiments under natural long day or controlled short day conditions revealed a single flowering time QTL. This gene had an additive effect of 1–6 days, depending on environment and genetic background, and was mapped in both populations to a position in the region of marker Xbarc164 near the centromere on the long arm of 3B. Comparisons of the genetic maps with other 3B maps developed by the authors indicated that the QTL may be homologous to a QTL segregating in UK germplasm.     

Bahiagrass crop coefficients from eddy correlation measurements in central Florida

Bahiagrass (Paspalum notatum) is a warm-season grass used primarily in pastures and along highways and other low maintenance public areas in Florida. It is also used in landscapes to some extent because of its drought tolerance. Bahiagrass can survive under a range of moisture conditions from no irrigation to very wet conditions. Its well-watered consumptive use has not been reported previously. In this study, bahiagrass crop coefficients (K _c) for an irrigated pasture were determined for July 2003 through December 2006 in central Florida. The eddy correlation method was used to estimate crop evapotranspiration (ET_c) rates. The standardized reference evapotranspiration (ET_o) equation (ASCE-EWRI standardization of reference evapotranspiration task committee report, 2005) was applied to calculate ET_o values using on site weather data. Daily K _c values were estimated from the ratio of the measured ET_c and the calculated ET_o. The recommended K _c values for bahiagrass are 0.35 for January–February, 0.55 for March, 0.80 for April, 0.90 for May, 0.75 for June, 0.70 for July–August, 0.75 for September, 0.70 for October, 0.60 for November, and 0.45 for December in central Florida. The highest K _c value of 0.9 in May corresponded with maximum vapor pressure deficit conditions as well as cloud free conditions and the highest incoming solar radiation as compared to the rest of the year. During the summer (June to August), frequent precipitation events increased the cloud cover and reduced grass water use. The K _c annual trend was similar to estimated K _c values from another well-watered warm-season grass study in Florida.     

Evaluating eddy covariance cotton ET measurements in an advective environment with large weighing lysimeters

Eddy covariance (EC) systems are being used to assess the accuracy of remote sensing methods in mapping surface sensible and latent heat fluxes and evapotranspiration (ET) from local to regional scales, and in crop coefficient development. Therefore, the objective was to evaluate the accuracy of EC systems in measuring sensible heat (H) and latent heat (LE) fluxes. For this purpose, two EC systems were installed near large monolithic weighing lysimeters, on irrigated cotton fields in the Texas High Plains, during the months of June and July 2008. Sensible and latent heat fluxes were underestimated with an average error of about 30%. Most of the errors were from nocturnal measurements. Energy balance (EB) closure was 73.2–78.0% for daytime fluxes. Thus, daylight fluxes were adjusted for lack of EB closure using the Bowen ratio/preservation of energy principle, which improved the resulting EC heat flux agreement with lysimetric values. Further adjustments to EC-based ET included nighttime ET (composite) incorporation, and the use of ‘heat flux source area’ (footprint) functions to compensate ET when the footprint expanded beyond the crop field boundary. As a result, ET values remarkably matched lysimetric ET values, with a ‘mean bias error ± root mean square error’ of −0.03 ± 0.5 mm day⁻¹ (or −0.6 ± 10.2%).     

Estimation of actual evapotranspiration for a drip-irrigated Merlot vineyard using a three-source model

A study was performed in order to evaluate the three-source model (Clumped model) for direct estimation of actual evapotranspiration (ET_a) and latent heat flux (LE) over a drip-irrigated Merlot vineyard trained on a vertical shoot positioned system (VSP) under semi-arid conditions. The vineyard, with an average fractional cover of 30%, is located in the Talca Valley, Region del Maule, Chile. The performance of the Clumped model was evaluated using an eddy covariance system during the 2006/2007 and 2007/2008 growing seasons. Results indicate that the Clumped model was able to predict ET_a with a root mean square error (RMSE), mean bias error (MBE), and model efficiency (EF) of 0.33, −0.15 mm day⁻¹ and 74%, respectively. Also, the Clumped model simulated the daytime variation of LE with a RMSE of 36 W m⁻², MBE of −8 W m⁻², and EF of 83%. Major disagreement (underestimated values) between observed and estimated values of ET_a was found for clear days after rainfall or foggy days, but underestimated values were less than 10% of the data analysis. The results obtained in this study indicate that the Clumped model could be used to directly estimate vine water requirements for a drip-irrigated vineyard trained on a VSP. However, application of the Clumped model requires a good characterization of the drip-irrigated vineyard architecture.     

Flow over rectangular sharp-crested weirs

Sharp-crested weirs are the simplest form of over-flow spillway that commonly used to determine the flow rate in hydraulic laboratories, industry and irrigation systems, where highly accurate discharge measurements are needed. In this study, the experimental upper and lower nappe profiles in rectangular sharp-crested weirs are fitted by quadratic and cubic equations, respectively. In addition, free-vortex theory is used to simulate flow over this kind of weirs and determine discharge coefficient. Physical models of sharp-crested weirs with various widths and heights were considered. The proposed method agrees well with the experimental observations. Also, the experimental data indicate that the suggested equation presents reasonable results for the range of 0 < h/P < 9.     

Optimizing yield,water requirements,and water productivity of aerobic rice for the North China Plain

Water resources for agriculture are rapidly declining in the North China Plain because of increasing industrial and domestic use and because of decreasing rainfall resulting from climate change. Water-efficient agricultural technologies need to be developed. Aerobic rice is a new crop production system in which rice is grown in nonflooded and nonsaturated aerobic soil, just like wheat and maize. Although an estimated 80,000 ha are cultivated with aerobic rice in the plain, there is little knowledge on obtainable yields and water requirements to assist farmers in improving their management. We present results from field experiments with aerobic rice variety HD297 near Beijing, from 2002 to 2004. The crop growth simulation model ORYZA2000 was used to extrapolate the experimental results to different weather conditions, irrigation management, and soil types. We quantified yields, water inputs, water use, and water productivities. On typical freely draining soils of the North China Plain, aerobic rice yields can reach 6–6.8 t ha⁻¹, with a total water input ranging between 589 and 797 (rainfall = 477 m and water application = 112–320 mm). For efficient water use, the irrigation water can be supplied in 2–4 applications and should aim at keeping the soil water tension in the rootzone below 100–200 kPa. Under those conditions, the amount of water use by evapotranspiration was 458–483 mm. The water productivity with respect to total water input (irrigation plus rainfall) was 0.89–1.05 g grain kg⁻¹ water, and with respect to evapotranspiration, 1.28–1.42 g grain kg⁻¹ water. Drought around flowering should be avoided to minimize the risk of spikelet sterility and low grain yields. The simulations suggest that, theoretically, yields can go up to 7.5 t ha⁻¹ and beyond. Further research is needed to determine whether the panicle (sink) size is large enough to support such yields and/or whether improved management is needed.     

Determination of the crop coefficient for grafted ‘Tahiti’ lime trees and soil evaporation coefficient of Rhodic Kandiudalf clay soil in Sao Paulo,Brazil

The expansion of permanent trickle irrigation systems in Sao Paulo (Brazil) citrus has changed the focus of irrigation scheduling from determining irrigation timing to quantifying irrigation amounts. The water requirements of citrus orchards are difficult to estimate, since they are influenced by heterogeneous factors such as age, planting density and irrigation system. In this study, we estimated the water requirements of young ‘Tahiti’ lime orchards, considering the independent contributions from soil evaporation and crop transpiration by splitting the crop coefficient (Kc = ETc/ETo) into two separate coefficients; Ke, a soil evaporation coefficient and Kcb, a crop transpiration coefficient. Hence, the water requirement in young ‘Tahiti’ lime (ET_y) is ET_y = (Ke + Kcb) · ETo, where ETo is the reference crop evapotranspiration. Mature tree water requirement (ET_m) is ET_m = Kcb · ETo, assuming no soil water evaporation. Two lysimeters were used; one was 1.6 m in diameter and 0.7 m deep, and the other was 2.7 m in diameter and 0.8-m deep. The first one was used to calculate evaporation and the second one was used for transpiration. ETo was estimated by the Penman–Monteith method (FAO-56). The measurements were conducted during a period between August 2002 and April 2005 in Piracicaba, Sao Paulo state, Brazil. The lysimeters were installed at the center of a 1.0-ha plot planted with ‘Tahiti’ lime trees grafted on ‘Swingle’ citrumelo rootstock. The trees were 1-year old at planting, spaced 7 × 4 m, and were irrigated by a drip irrigation system. During the study period, Kc varied between 0.6 and 1.22, and Kcb varied between 0.4 and 1.0. The results suggested that for young lime trees, the volume of water per tree calculated by Ke + Kcb is about 80% higher than the volume calculated using Kc. For mature trees, the volume of water per tree calculated using just Kcb can be 10% less than using Kc. The independent influence of soil evaporation and transpiration is important to better understand the water consumption of young lime trees during growth compared to mature lime trees.     

‘Microbigation’: delivery of biological control agents through drip irrigation systems

The uniform and precise application of microbial particles close to the target organism and to the plant to be protected can increase the success of a biological control treatment. The use of systems or technologies which are usually available in agriculture could influence the acceptability of biocontrol agents by farmers, and enlarge the market. A pilot system was realized using dripper lines, drippers, filters and other tools commonly used in irrigation and precision agriculture in the greenhouse to evaluate their suitability for applying and distributing microbial biocontrol agents. Conidial suspensions of marketed or marketable agents were used, i.e. Fusarium oxysporum, F. solani, Trichoderma harzianum and Paecilomyces lilacinus. The experiments carried out demonstrated that conidial suspensions (10⁶ conidia ml⁻¹) can pass through the drippers without causing clogging, regardless of their size, and remained viable. The term ‘microbigation’ is here proposed for this kind of microbial application technique.     

Physiological,morphological, chemical and genomic diversities of different origins of thyme (<Emphasis Type="Italic">Thymus vulgaris</Emphasis> L.)

For the selection of donors with valuable characteristics for breeding, 39 thyme accessions were evaluated in three years according to a staggered schedule. The criteria investigated were: winter hardiness, beginning of flowering, growth height, yield of the dry herb, content of essential oil, composition of the essential oil, DNA content of cell nuclei and number of chromosomes. The most strongly varying traits between the populations were the yield of dry herb, the content of essential oil and the content of volatile phenols with coefficients of variation (CV) between CV 40% and 50%. The largest variation within a population was detected for the yield of dry herb (CV 25–46%) and the content of essential oil (CV 17–48%). The homogeneity of the populations was different. The minimal average coefficient of variation of all traits (CV 19%) was determined in the population of the cultivar ‘Varico II’ and in a population from Lithuania. The ploidy level of T. vulgaris was diploid (2n = 30).