论青海地区育苗技术研究

培育苗木的一般流程,是从采种到种子发芽,再将已发芽种子移入容器。我们经常看到有部分的人工林呈现出成活率低、树形差,以及生长参差不齐等状况,这些问题很多是在幼苗期就存在了。因此,林业如能在此起始点上把好关,那么在后续的经营上就可以节省费用支出。青海地区属于高原气候,林业的育苗工作有自己的一系列特色。本文以云杉育苗为例,首先分析了林业育苗的一般思路,进而通过对青海地区云杉育苗试验的解析,阐明青海地区林业育苗技术要领。     

竹材木质素高效降解菌的分离筛选及鉴定

采用愈创木酚变色、苯胺蓝平板退色和酶活性测定筛选出高效降解竹材木质素的菌株。结果表明:通过腐烂的竹材等34份样品分离纯化出139株真菌;通过0.04%愈创木酚和0.1‰苯胺蓝平板初筛,从分离纯化的139株真菌中筛选出具有产木质素降解酶活性的7株降解菌;7株降解菌经液体产酶培养基复筛得到2株高效产降解木质素酶活的菌株:培养7 d,漆酶、木质素过氧化物酶和锰过氧化物酶活性分别达72.558、23.769、55.044和34.611、38.781、82.646 U/mL;2株降解菌FG-35和FG-98分别鉴定为革耳菌Panus lecomtei和烟管菌Bjerkandera adustus。     

简易葡萄酒酿造过程中存在的问题及解决办法

指出了在简易葡萄酒酿造过程中,容易出现如风味较差、酒精度低、甲醇含量较高、产率低、易产生杂菌等问题,探讨了改善酿造的工艺,并为简易葡萄酒酿造提供了相应的解决方法。     

南昌市旅游文化创意产业发展路径探析

指出了旅游业与文化产业的有机融合成为近10年来我国文化领域和旅游领域共同发展的重大成果,到2020年,文化产业将成为国民经济支柱性产业。江西南昌是中国革命的发祥地,文化资源丰富,具备发展文化创意产业的条件。提出了应对的策略为：依托地方政策,加强旅游业与其他创意产业的融合,走文化资源的持续开发与品牌策略,加强旅游文化创意经营人员培育。     

AFLP-based genetic linkage map of marine shrimp Penaeus (Fenneropenaeus) chinensis

This paper presents the genetic linkage map of the Chinese shrimp Penaeus (Fenneropenaeus) chinensis constructed with 472 AFLP markers. A hundred F₁ progeny from an intercross between a female from the new variety “Yellow Sea No. 1” and wild caught male used for the mapping study. Two separate maps were constructed for each parent. The female linkage map consisted of 197 marker loci forming 35 linkage groups and spanned a total length of 2191.1 cM, with an average marker space of 13.5 cM. The male map consisted of 194 marker loci mapped to 36 linkage groups and spanned a total length of 1737.3 cM, with an average marker spacing of 11.0 cM. The level of segregation distortion observed in this study was 12.2%. The estimated genome length of P. chinensis was 3150.3 cM for the female and 2549.3 cM for the male, respectively. The observed genome coverage was 69.6% for the female and 68.1% for the male map. The linkage maps constructed in this study provide basic information for further linkage studies on Chinese shrimp, and more importantly, the construction of the maps are part of the work of the genetic breeding programs which will be used for growth discovered in the QTL analysis of P. chinensis.     

Effect of feeding on the tissue free amino acid concentrations in rainbow trout (Oncorhynchus mykiss Walbaum)

This study investigates whether tissue free amino acid (FAA) pools in rainbow trout,Oncorhynchus mykiss (Walbaum), are altered following feeding and the relationships between the amount of food consumed and the FAA pool size. Trout were starved for 7 days to provide baseline data and then refed on day 8. Individual food intake was measured by radiography and the consumption of amino acids (AA) calculated from dietary protein consumption. Total FAA concentrations in the stomach, liver and white muscle were little changed at various times after the meal and this pattern was repeated for the majority of individual FAA. Overall, the most notable change was a reduction in essential FAA concentrations (principally in valine, leucine and isoleucine) in the white muscle following feeding. However, in the caeca total FAA, total essential FAA and a number of individual FAA were significantly elevated at 4, 9 and 15h following feeding. There were few significant correlations between dietary amino acid consumption and total tissue FAA and essential FAA concentration in the stomach, caecum and white muscle; correlations were stronger in the liver. In order to explain the relative constancy of total FAA concentrations in the tissues following food intake (which represents over 100% of the total FAA pool) a model is presented that quantifies the AA flux through the free pools and considers the role of protein turnover in regulating FAA pool size.     

我国兽医器械产业市场分析

从兽医器械市场规模、市场结构、供需分析、关键经济指标、进出口等产业经济方面对我国兽医器械产业进行分析评价。结果表明,近几年我国兽医器械市场规模逐年扩大,供需基本处于平衡状态;产业盈利能力总体向好,负债水平稳步回落;进出口逆差仍将长期存在,但已呈现逐渐缩小趋势。总的来说,我国兽医机械产业市场日趋活跃,且有较大的发展空间。     

基于16srDNA序列分析冬樱花花期蜜囊细菌多样性

对昆明冬樱花花期东、西方蜜蜂蜜囊细菌进行分离、纯培养;并对分离菌株的16srDNA基因片段进行扩增和测序,序列比对后进行系统发育分析。结果表明:东方蜜蜂蜜囊细菌包括3个纲中的10个种,西方蜜蜂蜜囊细菌包括3个纲中的13个种,花蜜细菌包括4个纲中的6个种,所采集样品除Bacillus subtili相似以外,其余菌种差异较大。     

三角帆蚌蚌瘟病原的初步探讨

探讨了三个水产场三角帆蚌大批死亡的病因。取病料经匀浆、离心后取上清液,再经青霉素、链霉素处理或微孔滤膜过滤除菌,人工感染健康三角帆蚌均能引起发病死亡,并能连续传代。其症状和病理解剖变化与自然发病的蚌相似。病原病毒粒子有囊膜,核衣壳呈二十面体对称,大小80～120nm,暂称为蚌的类疱疹病毒。含毒病料置50％甘油磷酸盐缓冲液中,在0～4℃冰箱保存6个月,-20℃低温冰箱中保存一年半,仍有致病力。