遵义市辣椒产业发展的现状、问题与对策

本文概述了遵义市辣椒产业发展的基本情况及存在的问题,进一步提出了当前遵义辣椒产业发展的思路与措施。     

赤琥珀螺线粒体全基因组测定与系统发育关系的研究

旨在通过高通量测序技术获得赤琥珀螺（Succinea erythrophana）线粒体基因组全序列，对其结构及组成特征进行分析，并结合NCBI数据库已公布的琥珀螺科及柄眼目物种序列，以最大似然法和贝叶斯法分别进行系统发育分析。结果显示：①赤琥珀螺线粒体基因组全长 14 023 bp（GenBank No.ON533899），由37个基因和一段富含AT的非编码控制区组成，有20处基因间隔，13处基因重叠。A＋T平均含量为77.3%，表现出明显的AT偏向性。基因的排列顺序、结构与组成、密码子使用情况与琥珀螺科已报道种类相似。 ②除tRNA-Phe、tRNA-His、tRNA-Ser1、tRNA-Ser2外，其余 tRNAs呈典型三叶草结构。③Ka/Ks选择压力分析显示其受到纯化选择作用。④系统发育研究揭示赤琥珀螺与同属的腐败琥珀螺（Succinea putris）亲缘关系最近，然后与同科其他物种形成姐妹群关系。     

四倍体刺槐的饲用营养价值分析

该研究测定了四倍体刺槐常规营养成分、微量元素、维生素、氨基酸、单宁等的含量,并与苜蓿、羊草等作了对比.四倍体刺槐叶粉干物质中的粗蛋白含量达到24.6%,粗纤维含量为13.6%,可作为蛋白质饲料使用.综合各项指标,认为四倍体刺槐是一种较好的粗饲料,可在反刍动物饲料中替代苜蓿.     

板栗良种基地营建技术研究

在江苏省溧阳市龙潭林场，进行了板优良品种的收集，良种优良，良种繁育，板栗嫩枝扦插等一系列营建技术研究，取得了明显的经济，社会效益。     

Study on Proliferation and Apoptosis of EBL Cells Induced by P48 Protein of Mycoplasma bovis

The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis.     

基于元共祖的基因组联合育种模拟研究

旨在将整合元共祖的一步法(single-step genomic best linear unbiased prediction with metafounders,MF-SSGBLUP)应用到基因组联合育种中,并与其他经典基因组选择方法进行比较分析。本研究使用QMSim软件模拟3个系谱相互独立的奶牛群体;分别使用广义最小二乘法(generalized least squares,GLS)和原始方法(naïve,NAI)估计不同群体间的祖先关系矩阵Γ;将MF-SSGBLUP、SSGBLUP和BLUP用于3个模拟群体的联合育种,评估各方法在遗传参数和育种值估计方面的差异。在不同遗传力下,GLS所得的Γ矩阵在对角线元素上略低于NAI法,在非对角线元素上没有明显差异,且基因组关系矩阵与基于元共祖构建的亲缘关系矩阵对角线元素相关系数(0.750~0.775)高于基因组关系矩阵与传统的亲缘关系矩阵相关系数(0.508~0.572)。MF-SSGBLUP遗传力估计值(0.138、0.140、0.297和0.298)与当代群体遗传力(0.107和0.296)的偏差小于其余两种方法(0.145、0.173、0.273和0.340),且MF-SSGBLUP估计育种值准确性(0.888~0.908)高于SSGBLUP法(0.863~0.876)和BLUP法(0.854~0.871)。表明,MF-SSGBLUP的遗传参数估计值无偏性更好,估计育种值准确性更高。根据上述模拟数据结果表明,在联合育种中,整合元共祖的基因组选择方法优于其他经典基因组选择方法。     

秦岭松栎林带生物量及其营养元素分布特征

秦岭脱齿栎林、油松林和华山林（包括０－６０cm土层）营养元素总贮量达２９．２５００－３９０．１７３９t.hm^-2，其大小为锐齿栎林＞华山松＞油松林，土壤中营养现贮量占系统总量的９３．１０％－９９．３９％，植被层和凋落物层仅占０．５７％－３．００％和０．１０％－１．９９％；３林分乔木层生物量、木材蓄积量和营养元素积累量平均值分别为１２０．４８３t.hm^-2,129.384m^3.hm^-2和１２２６．９kg.hm^-2，锐齿栎林＞油松林＞华山松林。３林分林下植被层生物量现存量和营养元素积累量平均值分别为１．５５３t.hm^-2和５３．１kg.hm^-2，锐齿栎林最大，华山松林最小。林下凋落物现存量和营养元素积量平均值分别为１７．４７５t.hm^-2和５０２．５kg.hm^-2，３林分凋落物现存量、营养元素含量及其积累量都存在明显的差异。锐齿栎林、油松林和华山松林营养元素年吸收量、归还量、存留量和平均归还率分别为３３．４、１４７．２和２６４．３，１９５．６、６６．９和８４．１，１３８．８、８０．４和１８０．２kg.hm^-2和０．５８５、０４５４和０．３１８，３林分各营养元素的年吸收量，归还量和存留量也存在一定的差异，锐齿栎林干和皮中Ｃa和Ｍg含量远远高于油松林和华山松林的，锐齿栎林多代连作势必导致营养元素的大量流失，特别是Ｃa和Ｍg，会破坏土壤原有离子动态平衡，引起林地土壤理化性质改变，林地生产潜力逐渐下降。     

简述钢管混凝土

介绍了钢管混凝土结构的特点、研究现状及其工程应用。     

Analysis of the forest fires in the Antalya region of Turkey using the Keetch–Byram drought index

We investigated the correlation of large fires([300 ha) from 1992 to 2013 within the borders of the Antalya Regional Directorate of Forestry using the Keetch–Byram drought index(KBDI). Daily KBDI values were calculated for each year, and values for the period before the year 2000 differed significantly from those after2000. After 2000(large fires occurred in 2004, 2006, 2007,2008, 2010, and 2013), when KBDI values increased, the KBDI, but not the number of fires, was inversely correlated with the natural log of the burned area(NLBA). While there were both high and low KBDI values when the NLBA was small, only high KBDI values were associated with high NLBA values. Particularly for logarithmic values of 4 and higher, KBDI values increased in parallel with increases in NLBA values. On the basis of a Mann–Whitney U test done in addition to a Pearson correlation test, we found that when the burned areas were grouped according to small and large areas, the KBDI could be used to distinguish the two groups. Using a conditional probability analysis, we found that 4th, 5th and 6th class KBDI values may lead to large fires at the 60 % possibility.Similarly, the possibility of large fires greater than the median burned area in any given 6 years was found to be48 %. In addition, while the mean value of KBDI is 390.51 for the period from May to September for these 6 years, it is 359.93 for the other years. Consequently, the area burned also increased as the KBDI classes(Class 0: 0–99, Class 1:100–199, Class 2: 200–299, Class 3: 300–399, Class 4:400–499, Class 5: 500–599, Class 6: 600–699, and Class 7:700–800) increase.     

板栗扦插繁殖试验

从１９９２－－１９９５年扦插１６０００个插条，成活率由１９９２年的２６％提高到１９９５年的９６％。共繁殖共根苗２２３９株，移栽１０００余株，长势良好。试验认为（１）嫩枝扦插生根率高于硬枝，从实生苗母树采集的插穗经生根剂处理中生根。（２）扦插时间以５月下旬至７月中旬较适宜，６月中，一旬为最佳时间。（３）扦插基质以珍珠岩＋石砂最合适，（４）生根剂以ＰＧＣ，ＰＲＡ液态高深度速蘸法为最佳。