遗传背景对水稻无融合生殖材料C1001双胚苗频率影响的研究

同核异质雄性不育材料与无融合生殖材料Ｃ１００１Ｂ杂交和回交表明，Ｃ１００１Ｂ的双胚苗，是受核基因控制的性状，双胚苗频率的表达，具明显的胞质效应和胞核效应，在胞质效应中，不育胞质与可质胞质的差异尤其显著，不育胞质对双胚苗的表现有明显的抑制作用，不同世代间胞质效应和胞核效应的表现也不一致。此外，环境条件对Ｃ１００１的双胚苗表现有一定的影响。     

母猪饲料中添加中药“泻康宁”对仔猪黄白痢的治疗试验

由埃希氏大肠杆菌引起的仔猪黄白痢会导致机体水分和电解质大量丧失 ,使机体衰弱甚至死亡 ,从而造成很大的经济损失。目前 ,大肠杆菌引起的仔猪黄白痢已成为影响仔猪发育和育成的重要疾病之一 ,成为养猪业的一大障碍。虽然应用抗生素、磺胺类药物和抗菌增效剂等治疗仔猪黄白痢有一定的疗效 ,但由于大肠杆菌具有很强的变异性 ,易产生抗药性而影响治疗效果 ,而且 ,有的药物还会在体内蓄积 ,给人类的健康带来危害 ,正因为如此 ,人们开始寻找新的无公害的药物。实验研究发现 ,中草药不仅能够促进畜禽生长发育和提高生产性能 ,调节动物机体的新陈…     

重视粪污处理 改善养殖环境

近20多年来，我国养殖业发展迅速，取得了可喜的业绩。我国肉类、蛋类总产量连续多年保持世界第一，养殖业为改善我国人民生活水平，调整人们膳食结构、提高农民收入做出了巨大的贡献。但它的发展不可避免地产生了大量的“畜产公害”，畜禽粪便、养殖污水任意堆弃、排放现象普遍存在。     

供水工程影响下中国北方地区耕地后备资源开发潜力

灌溉水源是北方地区耕地后备资源能否有效开发的关键。当前,中国正在推动国家水网工程规划建设,为北方地区耕地后备资源开发提供了新的机遇。在以往研究基础上,该研究考虑北方地区已建、在建及规划的供水工程,基于自然适宜、利用高效、发展稳定三方面评价准则,结合三维魔方空间分类方法,开展了雨养情景和供水灌溉情景下耕地后备资源开发潜力分析。结果表明：雨养农业情景下,北方地区勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积分别为2.9万、1.0万和0.6万km²。灌溉农业情景下,耕地后备资源面积明显增加,勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积将分别达到5.4万、7.6万和6.5万km²,主要集中于新疆、内蒙古和甘肃3省（自治区）。该研究结果可为中国耕地后备资源开发利用及国家水网工程效益发挥提供参考。     

Biological characteristics of long passaging rat mesenchymal stem cells

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.     

Preparation of gfp-bcl-XL-contained recombinant adenovirus vector by the homologous recombination in bacteria

AIM: To prepare gfp-bcl-X_L-contained recombinant adenovirus(rAd-gfp-bcl-X_L).METHODS: Bcl-X_L gene was amplified from pEGFP-C₃-bcl-X_L, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-X_L. Then pAdTrack-CMV-bcl-X_L was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-X_L and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-X_L into 293 cells. PCR test indicated that the recombinant Ad contained bcl-X_L gene. The titer of the purified rAd-gfp-bcl-X_L was 6.5×10¹² PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-X_L. This affords a good gene transfer vector for the gene therapy in human’s diseases.     

Effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts

AIM: To investigate the effects of PD98059 on the differentiation from mesenchymal stem cells to osteoblasts.METHODS: hMSC were separated from human marrow and expanded in cuture medium. hMSC were induced with dexamethasone, β-glycerophosphate, vitamin C which acted as osteoblast differentiation inducer. PD98059 was added into the osteoblasts induction medium. The cells were assayed with cell morphology, alkaline phosphatase (AP) activity and calcium deposition. RESULTS: The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. After induced with osteoblasts induction medium, the cells showed changes in cell morphology from spindle-shape to cuboidal and polygonal. The AP activity increased gradually and reached the peak in 12 days, then decreased. Many scattered tangerice calcium nodes were observed. PD 98059 significantly inhibited AP activity and calcium deposition in a dose-dependent manner. A striking observation of the present study was that a few adipocytes appeared in cultures that were treated with PD 98059 and osteogenic differentiation medium. CONCLUSION: These results indicated that osteogenic diferentiation from the hMSCs was related to the activation of the ERK.     

喀喇昆仑山盖孜河水文水资源特性分析

喀喇昆仑山河流自有水文资料记载以来 ,近 10年洪灾发生频率明显高于以往 ,人民的生命财产损失程度也大幅度增加。在分析了盖孜河流域的径流及气候多年变化过程后 ,认为洪灾频发的原因是近年来流域内降水及气温呈上升趋势 ,导致高山冰川退缩 ,河流径流增加 ,洪灾增大、频率增高 ;小区域多年气候变化过程从侧面印证了我国西北地区气候由暖干向暖湿变化。     

苹果梨的育种价值

该文指出,苹果梨综合性状优良,是很好的梨育种种质资源;以苹果梨及其后代为亲本已选育出30余个新品种,大大丰富了梨种质资源;苹果梨的大果、耐贮、小果心、抗寒等优良性状在后代品种中有较强的表现;以苹果梨与多倍体品种杂交后代为多倍体的比率较高。并展望了苹果梨在今后育种中的主要研究方向。