葛根素对松辽黑猪前体脂肪细胞成脂分化的影响

为探究葛根素对松辽黑猪前体脂肪细胞成脂分化的调控作用,在细胞诱导液中分别添加0、10、20、40、60和80 μmol/L葛根素进行成脂诱导分化,用油红O染色法和甘油三酯酶法检测脂肪细胞分化过程中脂滴聚集情况和甘油三酯含量以考察脂质沉积和分化效果,并确定葛根素的最佳添加浓度;用实时荧光定量PCR检测对照组(0 μmol/L)和最佳葛根素浓度添加组成脂标志基因细胞过氧化物酶体增殖物激活受体γ(PPARγ)、CCAAT-增强子结合蛋白α(C/EBPα)及成脂分化基因乙酰辅酶A羧化酶(ACC)、脂肪酸结合蛋白(FABP4)、应激蛋白(TRIB)和叉头框蛋白O1 (FOXO1)的mRNA的表达水平,用Western blotting检测PPARγ和C/EBPα的蛋白表达水平。结果表明,与对照组相比,20、40和60 μmol/L葛根素均显著增加脂滴和甘油三酯含量(P<0.05),且40 μmol/L葛根素效果最佳;实时荧光定量PCR结果表明,与对照组相比,40 μmol/L葛根素显著上调成脂标志基因PPARγ、C/EBPα及成脂分化基因ACC、FABP4、FOXO1和TRIB的表达(P<0.05);Western blotting结果显示,与对照组相比,40 μmol/L葛根素显著增加PPARγ蛋白表达(P<0.05)。综上所述,40 μmol/L葛根素能够促进松辽黑猪前体脂肪细胞的成脂分化和脂质沉积。     

松辽黑猪METTL23基因多态性及其与繁殖性状的关联分析

为探究松辽黑猪METTL23基因多态性及其与繁殖性状的关联性,试验选取178头松辽黑猪母猪为研究对象,利用Sanger直接测序法查找METTL23基因外显子1~5的单核苷酸多态性(SNP),使用SPSS 19.0软件分析METTL23基因SNP位点与松辽黑猪繁殖性状(总产仔数、产活仔数、断奶仔猪数、初生重、3周龄重、断奶重、乳头数)的关联性。结果显示,仅在松辽黑猪METTL23基因外显子4发现1个SNP位点,命名为A62G,在A62G位点上检测到3种基因型,分别是AA、AG和GG。GG和G分别为优势基因型和优势等位基因,且A62G位点处于Hardy-Weinberg平衡状态(P>0.05)。该位点的遗传纯合度(Ho)为0.6516,高于遗传杂合度(He,0.3484),说明其在松辽黑猪群体中的变异较小;多态信息含量(PIC)为0.2877,属于中度多态位点(0.25<PIC<0.5),说明该遗传标记能够提供一定量的遗传信息。关联分析结果表明,GG基因型个体总产仔数、产活仔数和断奶仔猪数均显著或极显著高于AA基因型个体(P<0.05;P<0.01),总产仔数还显著高于AG基因型个体(P<0.05);AG基因型个体断奶仔猪数显著高于AA基因型个体(P<0.05);初生重、3周龄重、断奶重、乳头数各基因型间差异均不显著(P>0.05)。综上所述,METTL23基因外显子4存在多态性位点A62G,该位点与松辽黑猪产仔数存在显著关联,可作为松辽黑猪产仔数的遗传标记,用于分子育种。     

乳酸菌噬菌体的分离及生物学特性鉴定

为了解乳酸菌噬菌体的生物学特性,并为制定乳酸菌发酵生产过程中噬菌体污染的防控措施提供试验数据和参考,本研究通过斑点试验和双层琼脂平板法,以干酪乳杆菌（L. casei） ATCC 393、戊糖乳杆菌（L. pentosus） KLDS 1.0413、短小乳杆菌（L. brevis） ATCC 367为指示菌从乳制品、泡菜样品中分离乳酸菌噬菌体,通过透射电镜观察噬菌体形态,噬菌体基因组限制性内切酶作用分析其包装机制,并进行宿主范围和一步生长曲线的测定,SDS-PAGE分析噬菌体结构蛋白,对获得的噬菌体进行生物学特性鉴定。结果显示,分离获得3株烈性乳酸菌噬菌体,依次命名为Lc、Lpen和Lbre。电镜结果显示,噬菌体Lc、Lpen均由多面体头部和非收缩性尾部组成,而噬菌体Lbre由多面体头部和收缩性尾部组成。根据形态学分析,Lc和Lpen属于长尾噬菌体科（Siphoviridae）,B1类,Lbre属于肌尾噬菌体科（Myoviridae）,A1类。噬菌体基因组经限制性内切酶作用后,核酸电泳结果显示,噬菌体Lc、Lpen基因组均为异质平末端,其包装机制属满头包装,即pac-型,而Lbre含有黏性末端,其包装机制属于cos-型。宿主范围测定结果显示,噬菌体Lc、Lbre对宿主专一,而Lpen宿主谱较广。一步生长曲线显示,噬菌体Lc、Lpen和Lbre潜伏期分别为60、45和150 min,裂解期分别为45、90和105 min,裂解量分别为47、24和30 PFU/cell。SDS-PAGE分析显示,噬菌体Lc结构蛋白有7个,主要结构蛋白分子质量约为50 ku;噬菌体Lpen和Lbre结构蛋白均有5个,主要结构蛋白分子质量分别约为55和50 ku。综上,本研究分离获得3株乳酸菌烈性噬菌体,并对其主要生物学特性进行鉴定,对了解乳酸菌噬菌体及后续乳酸菌噬菌体污染的防治提供了试验数据和理论依据。     

瘦肉型猪组合配套杂交效果研究

为探索瘦肉型猪组合配套杂交方法和效果而进行试验。试验采用HN121（温氏杜洛克猪）、HN212（法系皮特兰♂×温氏杜洛克♀）为父本,以HN204（温氏大白♂×美系长白♀）为母本,对HN323（HN121×HN204）和HN424（HN212×HN204）两个组合配套进行研究。结果表明,HN424组合与HN323组合相比母猪的窝产仔数、窝产活仔数、仔猪初生窝重、21日龄仔猪成活率、21日龄窝重等繁殖性状差异不显著（P>0.05）;HN424组合仔猪的体重、背膘厚降低5.63%（P<0.05）,瘦肉率提高4.95%（P<0.05）,眼肌面积增大33.08%（P<0.01）,肉色评分降低7.19%（P<0.05）,大理石纹评分降低11.30%（P<0.01）,滴水损失率提高10.63%（P<0.01）,其他遗传性状差异不显著;HN424组合与HN204二元母系相比,日增重提高9.26%（P<0.05）,背膘厚减少16.41%（P<0.01）,料肉比减少8.02%（P<0.05）;HN323组合与HN204二元杂交母系相比,日增重提高7.20%（P<0.05）,背膘厚减少11.43%（P<0.01）,料肉比降低5.35%（P<0.05）。说明采取四元杂组合和三元杂交组合均对“大×长”（HN204）二元杂交有显著的遗传改良作用,“皮×杜×长×大”四元杂组合可显著提高猪的瘦肉率、眼肌面积,但肉色、大理石纹、滴水损失率等肉质指标变差,建议在“皮×杜×长×大”四系组合配套杂交中加入能改良肉质基因的猪种。     

保温型刚性护面渠道设计

在刚性面层下设置一道性能良好的保温层来防止基土冻结,可以从根本上解决冻胀破坏的问题。这种带保温层的刚性护面渠道称为保温型刚性护面渠道,根据热工原理,提出了保温型刚性护面渠道横断面设计在构造方面的要求,进一步推导出计算保温层厚度的有关公式,并给出算例,还指出了需要进一步研究和探讨的问题。     

覆膜防沙治沙方法

沙漠、沙尘暴和沙化问题已经成为威胁人类生存的头号敌人，如何根治沙漠、沙尘暴和沙化是摆在人类面前的十分紧迫的任务。覆膜防沙治沙方法的研究和提出，成功地解决了人类面临的重大防沙治沙难题。为人类征服自然，征服沙漠树立了信心。介绍了覆膜防沙治沙的技术方式、方法、原理和覆膜栽培技术，供防沙治沙工程参考和推广。     

水资源系统与社会经济生态系统协调性评价

水资源系统与社会经济生态系统的协调性已越来越引起关注。首先利用主成分分析法,分别求出水资源系统与社会经济生态系统的综合评价指数,然后采用距离协调度模型得到两个系统间的协调度值,对两个系统的协调性进行了定量分析。以山东省为例,计算了2001-2012年两系统的协调度值。结果表明:两系统综合评价指数总体趋势上升;两系统发展虽然不同步,但协调性良好。     

基于模拟退火算法优化给水管线造价公式研究

给水管线造价公式的精确性对工程经济分析与给水管网优化的科学性和经济性有重大影响。通过五个地区的铸铁管综合单价数据,采用最小二乘法对各组数据进行多项式一至十次的拟合,确定了给水管线造价公式的最佳拟合次数为三次;进一步采用模拟退火算法对拟合的多项式参数进行优化计算,求解给水管线造价公式。结果表明:凭借模拟退火算法随机全局搜索模式以及不受函数性质影响的优势,能够克服传统算法难以求解多阶导数的困难,提高公式拟合精度。     

Research Lactobacillus rhamnosusFermented Herbal and Bacillus subtilis Complex on Immune Performance,E.coli Infection of Broiler

This experiment was designed to study the complex of Lactobacillus rhamnosus fermented herbal and Bacillus subtilis on White Feather broiler immunity performance and impact of Escherichia coli infection.360 one-day-old broiler chickens were randomly divided into 3 groups with 4 replicates in each group and 30 chickens per replicate.The pretrial period lasted for 7 d,and the experiment lasted for 35 d.The chickens in the group Ⅰ(positive control group) and group Ⅱ(negative control group)were all only fed a basal diet,group Ⅲ was test group,by additive 1% fermented herbal preparations,groups Ⅱ and Ⅲ were intraperitoneal injection of 1 mL E.coli at 35 d,broiler mortality,immune organ index,cecalmicroflora content,immunoglobulin levels,IL-2 and IL-6 content were tested.The results showed that injection of E.coli caused massive death of chickens,group Ⅱtook up to 75.00%,it was significantly higher than groups Ⅰ and Ⅲ (P < 0.05),the mortality in group Ⅲ was significantly lower than group Ⅱ(P < 0.05),was only 23.33%.Injection of E.coli maked spleen index and thymus index of group Ⅱincreased significantly (P < 0.05),the spleen index and thymus index of groups Ⅰ and Ⅲ were no significant difference (P > 0.05).E.coli counts was significantly decreased after injectionin group Ⅲ (P < 0.05),but the number of intestinal Lactobacilli of group Ⅲ was significantly increased (P < 0.05),and inhibited the propagation of E.coli,the counts of E.coli in groups Ⅰ and Ⅲ were no significant difference (P > 0.05).At 42 d,the sIgA of the intestinal fluid in group Ⅲ were higher than that of groups Ⅰ and Ⅱ with 11.99% and 36.56%,respectively(P < 0.05).The serum IgG concentrations of group Ⅲ was higher than that of groupsⅠand Ⅱwith 14.68% and 28.15%,respectively(P < 0.05).At 42 d,the IL-2 content of group Ⅱ was the lowest,it was significantly lower than group Ⅲ(P < 0.05),the IL-6 of group Ⅲ was significantly lower than group Ⅱ(P < 0.05).     

Acute and Sub-chronic Toxicity Test on Rats of a New Kind of Antidiarrheal Chinese Herbal Medicine Compound Preparation for Livestock

In order to understand the security of a new kind of antidiarrheal Chinese herbal medicine compound preparation for livestock,acute and sub-chronic toxicity test were conducted.Acute toxicity test used the largest drug dose method,20 Wistar rats were orally treated with the Chinese medicine compound preparation.In the sub-chronic toxicity test,80 rats were randomly divided into 4 groups with 20 rats in each group and orally given a dose of 3 000,1 500,750 and 0 mg/(kg·BW）of Chinese medicine compound preparation once a day for 30 days.The general clinical status was observed,rats weight were measured and the dose was adjusted every week during the test,after the test measured blood routine index,biochemistry index,and preceded the gross anatomy observation,weighing each major organs and calculated the viscera coefficient,and proceded main viscera histopathological observation between the high dose group and the control group.The acute toxicity results showed that every rat would be alive gavaged with the lethal dose（LD₅₀）of compound preparation larger 5 g/(kg·BW).The sub-chronic toxicity autopsy showed that except heart,lung,and testicles in individual rats appeared mild bleeding in the high dose group,the other dose group organs found no abnormal change.The haematological index showed except mononuclear cell rate（P<0.05),and hematocrit declined significantly（P<0.05）in the high dose group,all the indexes of the other groups were in the normal range,there was no significant difference from the control group.The test suggested the Chinese medicine compound preparation was no toxicity under the condition of this test according to acute toxicity classification standard of exogenous chemicals by WTO,there was no effect on the growth and development of rats in the sub-chronic toxicity test,and there was no chronic toxicity at least 1 500 mg/kg feeding conditions in short-term repeated application.