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Mitsuhiro IRIE Yoshinori TAKEUCHI Yuzo OHTAKE Hitomi SUZUKI Nao NAGATA Takuma MIYOSHI Yumiko KAGAWA Tetsushi YAMAGAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1535-1539
A 13-year-old spayed mixed-breed dog was diagnosed with a gastrointestinal
stromal tumor (GIST) after histopathological examination of an abdominal mass. Five months
after surgical resection of the tumor, we detected the recurrence of GIST with multiple
disseminated abdominal lesions. A sequence analysis of cDNA obtained from a biopsy of the
recurrent tumors revealed a mutation within exon 9 of the c-kit gene
(1523A>T, Asn508Ile), which has been shown to cause ligand-independent
phosphorylation of the KIT protein in GISTs and canine mast cell tumors (MCTs). Upon
detection of the recurrent tumors, we initiated treatment with imatinib mesylate (10
mg/kg, q 24 hr). After 2 months, the dog achieved complete remission. Our findings
indicate that canine GIST, and possibly MCT, may be responsive to molecular-targeted
therapy. 相似文献
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SHINSUKE TOMITA EIJI NAWATA YASUYUKI KONO YOSHIKATSU NAGATA CHAIRAT NOICHANA AKKADET SRIBUTTA TATSUYA INAMURA 《Weed Biology and Management》2003,3(2):117-127
Rainfed paddy cultivation predominates in north-east Thailand. Direct dry seeding is replacing transplanting rice cultivation to increase the frequency of successful plantings and to save labor. The present study clarified differences in weed vegetation of direct dry-seeded and transplanted paddy fields under rich, medium and poor water conditions from agricultural and ecological viewpoints. A large number of paddy fields from a wide range of climatic, topographic, soil and hydrological conditions were used. A survey was conducted every three weeks, and data regarding paddy cultivation, weeds and water conditions were collected. Expansion of direct dry seeding may increase species with in-between water adaptability to hygrophytes and mesophytes, and decrease hydrophytes and mesophytes. This characteristic was remarkable under poor water conditions. Rice cover was similar in either planting method under rich water conditions. However, cover of direct-seeded rice was significantly smaller than that of transplanted rice under medium and poor water conditions. Species diversity was higher in direct dry-seeded paddy fields than in transplanted paddy fields under medium and poor water conditions. Impact of direct dry seeding in the fields with rich water conditions on the vegetation is small in the context of maintaining rice production by transplanting and species diversity. However, direct dry seeding is mainly adopted in fields with poor water conditions. Impact of the changes in cultivating methods is large on rice productivity, species composition and species diversity under such conditions. Therefore, how to best use resource-poor fields may be the key to maintaining rice production and regional-level species diversity. 相似文献
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Abstract. Growth rates and fecundity of Brachionus plicatilis fed baker's yeast, a diatom, and three different flagellates, were monitored during a 15-day feeding trial. Identical growing conditions and equivalent food levels for the different diets were maintained during rotifer culture. The highest growth rate and mean fecundity were exhibited by rotifers fed Chlorella saccharophila , followed in decreasing order by those fed Isochrysis galbana (T-iso), Tetraselmis suecica, Saccharomyces cerevisiae and Thalassiosira pseudonana . Significant differences in contents of neutral lipids, polar lipids, sugars, glycogen, protein, ash and corresponding energy reserves in the resultant five groups of rotifers were diet dependent. Total available energy, about 15 kJ/g in rotifers fed S. cerevisiae and I. galbana (T-iso) was significantly different from the 14kJ/g in rotifers fed T. suecica and C saccharophila, and in turn from the 13kJ/g in rotifers fed T. pseudonana . 相似文献
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Y. MIYAKOSHI H. HAYANO H. OMORI & M. NAGATA J. R. IRVINE 《Fisheries Management and Ecology》2002,9(4):217-223
Densities of wild masu salmon, Oncorhynchus masou Brevoort, were investigated before (late-September) and after (mid-November) autumnal habitat shifts in a small river in Hokkaido, northern Japan. Abundance of instream cover habitat formed by coarse woody debris, submerged vegetation and undercut banks was quantified. Density of 1+ and older masu salmon was significantly correlated with cover abundance in autumn, although cover was not significant for density of 0+ fish, indicating that clear-cut reaches were utilized by 0+ masu salmon in autumn. In early winter, densities of 0+ and 1+ and older masu salmon were both correlated with cover availability. These results suggest that the amount of cover habitat is an important regulator for densities of juvenile masu salmon during winter. 相似文献
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Teruaki TOZAKI Aoi OHNUMA Mio KIKUCHI Taichiro ISHIGE Hironaga KAKOI Kei-ichi HIROTA Kanichi KUSANO Shun-ichi NAGATA 《Journal of Equine Science》2021,32(4):125
One method of gene doping in horseracing is administering of exogenous genetic materials, known as transgenes. Several polymerase chain reaction (PCR)-based methods have been developed for detecting transgenes with high sensitivity and specificity. However, novel designs for reference materials (RMs) and/or positive template controls (PTCs) are necessary for simultaneous analysis of multiple transgene targets. In this study, we designed and developed a novel RM for simultaneously detecting multiple targets via microfluidic quantitative PCR (MFQPCR). Twelve equine genes were selected as targets in this study. A sequence region including primers and probes for quantitative PCR was designed, and a 10 bp sequence was inserted to allow the RM to be distinguished from the original transgene sequences. The sequences of individual detection sites were then connected for 12 genes and cloned into a single plasmid vector. We performed fragment size analysis to distinguish between the PCR products of the original transgene sequence and those of the RM, enabling identification of RM contamination. PTCs diluted to 10,000, 1,000, 100, and 10 copies/µl with horse genomic DNA from RM were stably stored at 4°C for 1 year. As digital PCR enabled absolute quantification, the designed substances can serve as an RM. These findings indicate that the RM design and storage conditions were suitable for gene doping tests using MFQPCR. 相似文献
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Noriyuki NAGATA Hiroshi OHTA Nozomu YOKOYAMA Yong Bin TEOH Noboru SASAKI Kensuke NAKAMURA Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):872
Hyperplastic goblet cells and abundant mucus are significant characteristics of inflammatory colorectal polyps (ICRPs) in miniature dachshunds. In this study, selected mucin gene expressions and goblet cell proportions were evaluated in miniature dachshunds with ICRPs and in healthy dogs. Mucin 2 (MUC2) gene expression was not significantly different among the groups, whereas mucin 5AC (MUC5AC) gene expression was significantly higher in the polypoid lesions than in healthy colonic mucosa. Although the percentage of goblet cells in the upper crypt regions did not significantly differ between the groups, that in the lower crypt regions was significantly decreased in polypoid lesions. In conclusion, increased MUC5AC gene expression and goblet cell proportion changes may be associated with the pathogenesis of ICRPs. 相似文献
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Yamato SAJIKI Satoru KONNAI Reiko NAGATA Satoko KAWAJI Hayato NAKAMURA Sotaro FUJISAWA Tomohiro OKAGAWA Naoya MAEKAWA Yukinari KATO Yasuhiko SUZUKI Shiro MURATA Yasuyuki MORI Kazuhiko OHASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):162
Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease. 相似文献