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41.
Shota NISHIMURA Toshio WATANABE Atsushi OGINO Kazuhiro SHIMIZU Mitsuo MORITA Yoshikazu SUGIMOTO Akiko TAKASUGA 《Animal Science Journal》2013,84(1):1-7
Two taurine breeds, Japanese Black and Holstein, established from geographically distant origins and selected for different uses, beef and dairy, were extensively genotyped using a genome‐wide single nucleotide polymorphism (SNP) chip with more than 1000 animals of each breed. The genetic structure was examined by principal component analysis, in which the first principal component clearly separated the two breeds and explained more than 15% of the variance. Highly differentiated SNPs were detected throughout the genome, some of which were clustered within small regions on BTA4 (79.2–79.7 Mb, Btau4.0) and BTA26 (22.2–23.6 Mb). A breed assignment test was developed using 18 highly differentiated SNPs to distinguish Japanese Black from F1 (Japanese Black × Holstein) and Holstein. The error rate that an F1 or Holstein animal is misjudged as Japanese Black was expected to be < 0.8%, while the error rate that a Japanese Black animal is misjudged as F1 or Holstein was expected to be < 0.001%. This test provides a reliable and powerful method to detect breed label falsification in retail beef. 相似文献
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Acoustic Doppler current profiler (ADCP) data collected during routine monitoring surveys of the distribution and abundance of Japanese sardine larvae ( Sardinops melanostictus ) off the Pacific coast of Japan in February 1993 and 1994 were used to construct stationary average flowfields for three levels in the upper 100 m in each year. No large-scale meanders in the path of the Kuroshio Current were present in either year, but the axis of the current was closer to the coast in 1993 than in 1994. The flowfields were used to drive a particle-tracking model representing the dispersal of sardine eggs and larvae. Particles were released in accordance with the observed distribution of eggs, and their positions tracked for up to 40 days. In 1993, the model indicated that ≈ 50% of the egg production was carried north-eastwards out of the survey area into the area of the NW Pacific referred to as the Kuroshio Extension Zone. In contrast, only 5% of the egg production was exported to the Extension Zone in 1994, the remainder being retained in Japanese coastal waters. The consequences of the different dispersal patterns are discussed in relation to subsequent recruitment to the sardine stock. Based on commercial catch data, survival of the 1993 year class was 15% of that for the 1994 class. Hence, the results indicate that export of larvae to the Kuroshio Extension cannot in itself lead to successful recruitment. 相似文献
44.
Evidence of a local spawning site of marine eels along northeastern Japan, based on the distribution of small leptocephali 总被引:3,自引:0,他引:3
YOBUO KIMURA MICHAEL J. MILLER GEN MINAGAWA SHUN WATANABE AKIRA SHINODA JUN AOYAMA TADASHI INAGAKI KATSUMI TSUKAMOTO 《Fisheries Oceanography》2006,15(2):183-190
Evidence of recent spawning by several species of marine eels of the families Congridae, Ophichthidae, and Nettastomatidae was found over the continental shelf along the coast of northeastern Japan in October 2003. Sixty‐three leptocephali of at least three taxa that ranged from 3.8 to 12.3 mm total length were collected in a relatively small area, just to the south of Cape Shioya, primarily over the 100‐and 200‐m‐depth contours. The leptocephali of Gnathophis nystromi nystromi were the most abundant, followed by those of G. nystromi ginanago. This spawning area is located in an area that appeared to include water originating from the Tsugaru Warm Current and is further south than the typical latitude of the cold water of the Oyashio First Intrusion. These findings suggest that some species of marine eels may not migrate past the slope to spawn, and that the oceanographic features of the region may provide favorable conditions for the growth and retention of leptocephali in the area to the south of Cape Shioya. 相似文献
45.
Taiki TERAJIMA Anzu SHIBAHARA Yoshiaki NAKANO Shohei KOBAYASHI John R GODWIN Kentaro NAGAOKA Gen WATANABE Hideshige TAKADA Kaoruko MIZUKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1551
Persistent organic chemicals are non-biodegradable in nature and have a tendency to bioaccumulate in the top organisms of the food chain. We measured persistent organic chemicals, including polychlorinated biphenyls (PCBs), dichlorodiphenyldichloroethylene (DDE), and benzotriazole-based ultraviolet stabilizers (UV-BTs), in the serum of captive king penguins (Aptenodytes patagonicus) using gas chromatography with an electron capture detector and mass spectrometry to examine their age-related accumulation. PCBs, DDE, UV-PS, and UV-9 were detected in the blood of captive king penguins, and the concentrations of total PCBs, DDE, and UV-9 were positively correlated with age. These results suggest that there is a similar age-related accumulation of persistent organic chemicals in marine birds in the wild, and that older individuals are at a higher risk of contamination. 相似文献
46.
Ryo SATO Kanami WATANABE Ryo KAMATA Kazuki TAKEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(6):804
Anticoagulant chemicals (ACCs) such as warfarin are widely used in medical applications as well as for their rodenticide properties. Their efficacy is greatly influenced by polymorphisms in the gene encoding vitamin K epoxide reductase (VKOR). Evaluation of the activity of ACCs toward VKOR variants is essential to determine their proper use. Presently, this is achieved by co-expressing VKOR of Rattus Norvegicus and human clotting factor IX in cultured cells and measuring inhibition of vitamin K-dependent gamma-glutamyl carboxylation of factor IX (glaFIX) activity. However, glaFIX has only been quantified using indirect methods like blood coagulation assays. We have developed a sandwich enzyme-linked immunosorbent assay using a glaFIX-specific antibody to quantify glaFIX and used this to analyze inhibition of VKOR activity by warfarin. 相似文献
47.
Takeshi WATANABE Vita Ratri CAHYANI Jun MURASE Eiji ISHIBASHI Makoto KIMURA Susumu ASAKAWA 《Soil Science and Plant Nutrition》2009,55(1):73-79
Methanogenic archaeal communities inhabiting the paddy field soils in the Kojima Bay polder were investigated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), real-time PCR and sequencing analyses. Soil samples of the plow and subsoil layers were collected in 2006 from four paddy fields that were reclaimed between 1692 and 1954. The DGGE band patterns of the targeted 16S rRNA genes amplified from the extracted DNA from the samples were different from the patterns from the paddy field soils in diluvial and alluvial areas. The numbers of targeted 16S rRNA genes, which were involved with methanogenic archaeal and other archaeal sequences, were approximately 107 –108 and 106 g−1 dry soil in the plow and subsoil layers, respectively. Sequences of methanogenic archaeal 16S rRNA genes belonging to Methanocellales (Rice cluster I), Methanosarcinales and Methanobacteriales were obtained from the major DGGE bands. Whereas sequences in Methanomicrobiales, which were predominant methanogens in the diluvial and alluvial paddy fields, were not recovered. Known halophilic and methylotrophic methanogens, which are characteristic of saline and marine environments, were not detected. These results indicate that distinctive methanogenic archaeal communities have developed in the paddy field soils in the Kojima Bay polder. 相似文献
48.
稻田环境植物多样性研究 总被引:9,自引:1,他引:9
选择稻田灌溉水无工业废水污染的禹山坞和受工业废水污染的城北村两地进行稻田环境植物多样性调查。禹山坞稻田普遍使用化学除草剂,杂草种数明显少于城北村,水竹叶成为禹山坞稻田的优势种;城北村稻田未用除草剂及低洼积水环境保护了槐叶萍、满江红、轮藻和尖头丽藻等。禹山坞的畦畔植物种数显著高于城北村。城北村灌溉水质差有利于部分耐性植物蔓延形成优势种,如空心莲子草的强烈竞争,使部分竞争力弱的植物逐步消失,植物种数减少。两村的沟渠植物种类差异明显。 相似文献
49.
Yusuke TANAKA Yuka TAGAINO Shotaro NAKAGUN Takayuki MINESHIGE Kenichi WATANABE Hisashi INOKUMA Yoshiyasu KOBAYASHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(12):1852
A male Holstein-Friesian calf was born with multiple, cauliflower-like, pale pink cutaneous masses on the head and limbs. On histopathological examination, the cutaneous masses were diagnosed as congenital cutaneous fibropapillomatosis. Those lesions involved focal proliferation of sebaceous gland in the dermis. There were no histological findings to suggest bovine papillomavirus infection, such as the presence of intranuclear inclusion bodies, large keratohyalin granules, and koilocytosis. Furthermore, papillomaviral antigens and DNA were not detected by immunohistochemistry and polymerase chain reaction, respectively. These results suggested that there was no association between these cutaneous lesions and bovine papillomavirus infection, and the lesions were considered as harmartomatous changes. 相似文献
50.