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Noritaka ADACHI Daisuke YAMAGUCHI Akiyuki WATANABE Narumi MIURA Seiji SUNAGA Hitoshi OISHI Michiko HASHIMOTO Takatsugu OISHI Masaki IWAMOTO Hirofumi HANADA Masanori KUBO Akira ONISHI 《The Journal of reproduction and development》2014,60(2):100-105
The objective of this study was to examine the health and meat production of cloned sows
and their progenies in order to demonstrate the application of somatic cell cloning to the
pig industry. This study compared the growth, reproductive performance, carcass
characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies.
We measured their body weight, growth rate and feed conversion and performed a
pathological analysis of their anatomy to detect abnormalities. Three of the five cloned
pigs were used for a growth test. Cloned pigs grew normally and had characteristics
similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a
Landrace boar and used for a progeny test. F1 progenies had characteristics similar to
those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White
boar and used for the progeny test. F2 progenies grew normally. There were no biological
differences in growth, carcass characteristics and amino acid composition among cloned
sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies
showed normal reproductive performance. No specific abnormalities were observed by
pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs
had a normal karyotype. These results demonstrate that cloned female pigs and their
progenies have similar growth, reproductive performance and carcass quality
characteristics and that somatic cell cloning could be a useful technique for conserving
superior pig breeds in conventional meat production. 相似文献
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Masato MIYAKE Shinichiro HAYASHI Yoshikazu TAKETA Shunsuke IWASAKI Kouichi WATANABE Shyuich OHWADA Hisashi ASO Takahiro YAMAGUCHI 《Animal Science Journal》2010,81(2):223-229
Myostatin (MSTN) is a negative regulator during muscle differentiation, whereas insulin‐like growth factors (IGFs) are essential for muscle development. MSTN and IGFs act oppositely during myogenesis, but there is little information on the mutual relationship of MSTN and IGFs. The present study was conducted to examine whether MSTN affects IGF expression during early myogenesis in cattle. IGF‐1 mRNA was similarly expressed in M. longissimus thoracis of double‐muscled (DM) and normal (NM) Japanese shorthorn cattle. IGF‐2 mRNA expression was consistently higher in the normal and regenerating muscle of DM cattle than those of NM cattle. When myoblasts were isolated from regenerating M. longissimus thoracis, IGF‐2 mRNA expression showed a significant increase in differentiating DM derived myoblasts (DM‐myoblasts) as compared with differentiating NM derived myoblasts (NM‐myoblasts). An addition of recombinant mouse myostatin (rMSTN) to myoblast cultures attenuated IGF‐2 mRNA expression and decreased myotube formation, but did not effect IGF‐1 mRNA expression. An activin‐like kinase (ALK) inhibitor, SB431542, mediates MSTN action, suppressed the translocation of Smad2/3 into the nucleus in DM‐myoblasts, and restored the attenuated IGF‐2 mRNA expression and the decreased myotube formation induced by rMSTN in myoblast cultures. The findings indicate that MSTN may negatively regulate myoblast differentiation by suppressing IGF‐2 expression via ALK‐Smad signaling. 相似文献
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Tatsuyuki YOSHIDA Makoto SEKI Norio WATANABE Hiroki FURUTA Itaru YOSHIMURA Masahiro OSADA Koji CHIBA Konosuke OKADA Koh KAWASUMI Hitoshi USHIJIMA 《Animal Science Journal》2012,83(3):207-212
Diagnosis of corpus luteum (CL) function by rectal palpation (RP) has been widely used for recipient selection of embryo transfer (ET), a technology essential for genetic improvements in cattle. To examine the accuracy of RP diagnosis method, the relationship between RP‐based CL function and reproductive performance was compared in this study. In Experiment 1, CL of Holstein heifers on day 7 after estrus was classified into functional or hypoplastic by RP, and the results were compared with ultrasonographic (US) images and plasma progesterone (P4) levels. As a result, heifers with functional CL judged by RP had a mean maximum CL diameter of 20.1 ± 3.1 mm on US and a mean P4 concentration of 8.1 ± 2.3 ng/mL. These values were significantly greater than those of heifers with hypoplastic CL (12.4 ± 5.4 mm, 4.0 ± 2.8 ng/mL) (P < 0.001). In Experiment 2, the length of the estrus cycle was examined between functional CL and hypoplastic CL. The rate of heifers with a normal estrus cycle length with 18–25 days was significantly lower with hypoplastic CL than with functional CL (16/24 vs. 43/46, P < 0.01). In Experiment 3, 543 inseminated heifers were similarly classified by CL function by RP 7 days after estrus. The heifers with functional CL showed higher pregnancy rate compared with the heifers with hypoplastic CL (75.2 vs. 47.9%, P < 0.0001). Finally, the CL function of 66 heifers was examined by RP on day 7 post‐estrus, and ET was performed in 49 (74.2%) heifers with functional CL. As a result, 27 (55.1%) of them became pregnant. Taken together, these results reconfirm that RP on day 7 after estrus is useful for selection of heifers with functional CL. 相似文献
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Katsuji WATANABE 《Soil Science and Plant Nutrition》2009,55(1):42-52
The origin of soil protease in field soil was estimated using culture-dependent and independent approaches. Overall soil protease activity was much higher in field soils with an annual application of liquid livestock feces (120 t ha−1 year−1 and 600 t ha−1 year−1 ) compared with the activity recorded in other field soils, and the character of the soil proteases became highly homogeneous (approximately 70% metalloprotease in a 600 t field). Selective incubation studies suggested that bacteria were the most important source of soil protease. There were significantly higher correlations between serratial metalloprotease and the overall soil protease in both feces-applied fields in terms of the effect of inhibitors, and the bacteria, which produced serratial metalloprotease, were suggested to proliferate in both the 120 t and 600 t fields. The gene homologous to serratial metalloprotease gene was amplified in directly extracted DNA from field soils using selective DNA primer and proteolytic Serratia marcescens was certified to be one source of soil protease in these field soils. Proteolytic S. marcescens and its metalloprotease gene have occasionally been isolated and detected in field soils applied with raw feces, and have rarely been isolated or detected from other field soils. Proteolytic S. marcescens is believed to be introduced in the raw feces and subsequently colonizes the field soil and replaces the indigenous bacteria in the soil. 相似文献