论社会实践在艺术类大学生培养中的德育功效

在分析社会实践在艺术类大学生培养中的德育功效的基础上,重点对构建长效机制、探索社会实践和艺术实践的有机结合、完善激励评价和培训机制、充分利用校内外资源等方面提出了艺术类大学生社会实践活动工作的创新思路。     

桐庐县2005年中秋蚕减产原因分析与思考

蚕桑生产是桐庐县农村一项历史悠久的传统产业,尤其在分水地区,已成为农民经济收入的主要来源。全县现有桑园面积1730hm2,年饲养量5万余张,蚕桑产值4千余万元,蚕茧平均张产在40kg以上,随着茧丝行情上扬和省“蚕桑西进工程”在我县的实施,桑园面积增加400hm2,良种覆盖率得到提高     

The consensus oligonucleotide competitive binding with NF-κB inhibits TNF production

AIM:To investigate whether a consensus oligonucleotide, which contains nuclear factor-kappa B(NF-κB) binding site, decreases TNF production stimulated by lipopolysaccharide(LPS).METHODS:The thiophosphoric acid modified oligonucleotides were transferred into rat peritoneal macrophages directly, TNF content in LPS-stimulated cell culture supernatant was determined by ELISA.RESULTS:Adding the consensus oligonucleotides with NF-κB binding site into macrophages markedly decreased TNF production following LPS stimulation.CONCLUSION:These results show that use of the consensus oligonucleotides with NF-κB binding site, which can combine with NF-κB, can block or decrease TNF production induced by LPS in macrophages.     

苏云金芽孢杆菌YJ-2000菌株对家蚕安全性和杀虫活性的评价

应用时间-剂量-死亡率模型就苏云金芽孢杆菌(Bacillus thuringiensis)YJ-2000菌株对6种鳞翅目昆虫的杀虫活性作了初步评价。该菌株对家蚕基本无毒性,而对蔬菜害虫小菜蛾、菜粉蝶,水稻害虫三化螟、二化螟,桑树害虫桑螟幼虫均有不同程度的毒性,其中对小菜蛾和菜粉蝶的毒性最强;幼虫死亡率可高达100％。该菌株是对家蚕安全而对其它鳞翅目害虫具杀虫活性的特异性菌株,有望用于养蚕地区桑园及其邻近作物的鳞翅目害虫防治。     

回归最优设计进行无花果施肥的研究

应用回归正交设计中的二次饱和D-设计对无花果的合理施肥进行试验，寻求产量最大的施肥组合以及最佳经济效益的施肥肥料配比。     

扬子鳄大肠杆菌病的发生与防治

对人工养殖的 2 0 0 0余条扬子鳄所发生的大肠杆菌病进行治疗。结果表明 ,该病传播方式以内源性传播为主 ,在鳄体质不佳时即可爆发此病。如措施不当 ,最高发病率可达 90 %以上 ,死亡率达 30 %以上。     

MB43活疫苗免疫效果及对鸡法氏囊组织操作的研究

本试验对鸡传染性法氏囊病（IBD）MB43活疫苗免疫后鸡的囊体比、新城疫血凝抑制（ND-HI）抗体效价及IBD AGP效价进行检测。结果表明，含有IBD高母抗试验鸡在17日龄免疫MB43活疫苗后，囊组织有不同程度的损伤；对正常免疫的ND-HI抗体效价无影响；试验鸡16天后IBD AGP阳性率为100%；21天攻毒保护率100%。     

福建山地杉木大径材定向培育技术

在闽北、闽中山地，通过对杉木大径材培育过程中的遗传控制、密度控制、立地控制与林分施肥等主要措施的试验研究．确定了杉木大径材定向培育的优化方案，即选用杉木优良家系，选择18指数以上的立地，初植密度1800～2505株／hm^2，间伐后保留密度1100～1200株／hm^2，可不施肥。为杉木大径材的目标经营提供科学依据。     

Effect of 15(S)-hydroxyeicosate traenoic acid (15-HETE) on eNOS Thr495 phosphorylation in pulmonary artery endothelial cells

AIM: To examine the effect of 15(S)-hydroxyeicosate traenoic acid (15-HETE) on eNOS activity in pulmonary artery endothelial cells (PAECs).METHODS: 1.Rat pulmonary artery (PA) rings of intrapulmonary arteries were suspended under isometric tension in oxygenated Krebs buffer in order to observe the differences on constriction induced by 15-HETE using nitro-L-arginine methyl ester (L-NAME, 10-4 mol/L, an inhibitor of eNOS) and removal of endothelium.2.The bovine PAECs was cultured for NO assay by Greiss reaction.3.Immunoprecipitation (IP) and Western blotting (WB) were used to analyze the cell extracts from bovine PAECs treated with 2×10-6 mol/L 15-HETE for 30 and 60 min, using phosphatase-eNOS (Thr495) antibody for IP and eNOS antibody for WB.RESULTS: 1.Inhibition of eNOS with L-NAME and removal of endothelium significantly enhanced 15-HETE induced contractions in rat PA rings in a concentration-dependent manner (P＜0.05, P＜0.01).2.15-HETE increased phospho-eNOS (Thr495) levels (P＜0.01) in cultured bovine PAECs.3.10-6 mol/L 15-HETE (P＜0.05) did significantly reduce nitrite production, whereas 10-5 mol/L cinnamy l 3, 4-dihydroxy-［alpha］-cyanocinnamate (CDC, P＜0.05), 10-4 mol/L nordihydroguiairetic acid (NDGA, P＜0.01) significantly increased nitrite production, as measured by a spectrophotometric assay.CONCLUSION: 15-HETE inhibits eNOS activity and decreases production of NO (NO-2/NO-3).The pathway of eNOS/NO is involved in 15-HETE-induced contraction in the rat PA.     

胶体染料试纸条法快速诊断家畜血吸虫病的研究

为了适应现场家畜血吸虫病的筛查需要,设计了简便快速的胶体染料试纸条诊断技术用于检测家畜血清中的抗血吸虫抗体,以该方法检测血吸虫病牛、猪血清83份,其结果为阳性检出率100%,健康牛、猪血清73份的检测结果为阴性符合率分别为95.3%和94.0%,与感染肝片形吸虫的牛、羊血清16份的交叉反应率为18.8%.表明本方法敏感性高,特异性较强.