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Mitsuro KAMEYA-IWAKI Kimiaki MURAKAMI Shin-ichi ITO Kaoru HANADA Shuhei TANAKA 《Journal of General Plant Pathology》2000,66(1):64-67
Sequential transmission tests of Peanut stunt virus (PSV) and Cucumber mosaic virus (CMV) systemically infecting common bean, Phaseolus vulgaris, were conducted using Myzus persicae allowed to fast for 2 hr and then to acquisition feed on infected common bean plants or purified virus for 10 min. In the
sequential transmission tests using either one or 10 aphids per assay plant, three isolates of PSV (J,S,Y5) and one of CMV
(V) were transmitted from and to common bean up to a third or fourth inoculation access. Many aphids transmitted these viruses
to two or three plants. Purified viruses of PSV-S and CMV-V were also transmitted up to a third or second inoculation access
at low percentage. On tobacco, Nicotiana tabacum, aphids transmitted PSV-S and CMV-V only in the first inoculation access, although PSV-S was transmitted to only one plant
in the fourth and fifth inoculation access. These viruses may be transmitted in two phases by aphids, depending on the plant
species.
Received 16 April 1999/ Accepted in revised form 1 September 1999 相似文献
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The present study was conducted to determine the in vitro progesterone (P4) production by Mongolian gerbil (Meriones unguiculatus) corpus luteum (CL) on days 9, 18 and 22 of pregnancy in comparison with rat CL on days 9 and 16. The culture medium of one CL per well of a 96‐well plate was changed after 4 h incubation and the CL were further cultured for 24 h. The P4 in serum and in the medium were measured by enzyme immunoassay system. Gerbil CL weight measured after 24 h incubation was at the same level for all 3 days of pregnancy and significantly different from that of the rat CL for the 2 days of pregnancy. The serum P4 level in gerbils was high in early pregnancy and decreased in advanced days of pregnancy. In contrast, the P4 in rats was higher in the latter half of pregnancy. In vitro P4 production by a single CL after 4 h incubation decreased significantly during the days of pregnancy in both the gerbil and the rat. P4 values produced by the gerbil CL for 24 h incubation were significantly higher than after 4 h incubation in all 3 days of pregnancy, however, there was no difference between the two incubation time groups in the rat. In vitro P4 production by CL correlated well with the serum P4 level on the 3 days of pregnancy in the gerbil. In the rat, however, the relationship was inversed between the two parameters. The correlation in this experiment suggested that CL is the sole source of P4 for the maintenance of the gerbil pregnancy, with the result of abortion after an ovariectomy on day 20 of pregnancy as described in our previous report. 相似文献
186.
Chihiro TANAKA Kiminori SHIMIZU Abdul GAFUR Mitsuya TSUDA 《Journal of General Plant Pathology》2002,68(2):141-146
Six reddish brown polyxin-resistant mutants of Cochliobolus heterostrophus were isolated after ethyl methanesul-phonate and N-nitroquinoline oxide mutageneses followed by selection on polyoxin. All the mutants were highly resistant to polyoxin (MIC
> 1600 μg/ml). When mutants were crossed with the wild-type strain, all crosses had a 1 : 1 ratio of mutant (reddish brown
pigmentation and polyoxin resistance) : wild type (non-reddish brown pigmentation and polyoxin sensitivity), indicating that
the phenotypes in these strains were due to alteration at a single gene locus in each strain. Allelism tests revealed the
existence of two loci, Pol2 and Pol5. The results of the crossing and mutation-rate studies suggest that the each gene was pleiotropic for the reddish brown color
and polyoxin resistance.
Received 19 September 2001/ Accepted in revised form 25 December 2001 相似文献
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ABSTRACT: The present study estimated the age and growth of the silky shark Carcharhinus falciformis in the Pacific Ocean. Samples and biological data were collected from Japanese tuna longline and purse seine fisheries from 1992 to 1999. Vertebra centra were picked from 145 males and 153 females for age determination. The number of annual rings observed for males and females was 0–8 and 0–13, respectively. Combined sex von Bertalanffy growth equations were obtained as follows: Lt = 216.4(1 − e−0.148( t +1.76) ) where Lt is precaudal length in cm at age t . A mature size for males was considered to be approximately 135–140 cm (precaudal length), with an estimated age of 5–6 years, whereas corresponding values for females were 145–150 cm and 6–7 years, respectively. Birth size ranged from 48 to 60 cm. There was no remarkable difference in growth, birth size and age at maturity between the Pacific and Atlantic Oceans. The life history parameters of the silky shark are approximately the same in both oceans. 相似文献
189.
Ayat Omer ELKHALIL Yuko MAEJIMA Masato AOYAMA Hideyuki TANAKA Shoei SUGITA 《Animal Science Journal》2005,76(4):313-323
Morphological changes to and collagen loss from the rat uterus during postpartum involution were investigated. The expression patterns of collagen type III and myeloperoxidase (MPO) were also determined. Morphological changes were studied on days 1, 3, 5, 10, 15, 20, 22 and 25 postpartum. As a control, diestrus rats’ uterine were used. Specimens from the uterine horn were embedded in paraffin, cut into 8 µm coronal sections, and stained with hematoxylin‐eosin. The thickness of the longitudinal and circular smooth muscle layers and of the endometrium were measured. The collagen content was determined using hydroxyproline analysis. Immunostaining was used to examine the expression of collagen type III on days 1, 3, 5, 10, 15 and 20 postpartum; and MPO on days 1, 3, 5, 10 and 22 postpartum. The thickness of the smooth muscle layers was found to decrease rapidly postpartum: the circular smooth muscle layer returned to that of a non‐pregnant, control uterus by day 5 postpartum and the longitudinal smooth muscle layer by day 15 postpartum. Eosinophilic cells were observed in the endometrial stroma adjacent to the myometrium on days 10, 15 and 20 postpartum, and were confirmed as collagenous cells. Immunostaining identified collagen type III positive cells in the vessel‐rich layer adjacent to the placental site on days 1, 3, 5 and 10 postpartum, and these cells were confirmed to be phagocytic. Postpartum reduction in the weight of the uterus was accompanied by decreases in both the collagen content and the thickness of the longitudinal and circular smooth muscle layers. Furthermore, the phagocytic cells were shown to express MPO during postpartum involution of the uterus. 相似文献
190.
Yuko KAMIYA Mitsuru KAMIYA Masahito TANAKA Shigeru SHIOYA 《Animal Science Journal》2005,76(4):325-330
Eight pregnant heifers (primiparous cows) and seven pregnant cows in their second, third and forth pregnancies (multiparous cows) were assigned to two groups and fed either a low calcium (Ca) diet (Ca, 0.46%) or a high Ca diet (Ca, 0.86%) ad libitum from 3 weeks before the expected calving date to 3 days after parturition. All cows were examined for a change in dry matter intake (DMI), plasma minerals and bone turnover around parturition. The dietary Ca level did not affect the DMI in both primiparous and multiparous cows. The DMI of primiparous cows was significantly lower than that of multiparous cows (P < 0.05) in both the low and high dietary Ca groups. The dietary Ca level did not affect the concentrations of plasma Ca, phosphorus, magnesium and parathyroid hormone throughout the experimental period. Plasma phosphorus in primiparous cows was significantly higher (P < 0.05) than that of multiparous cows around parturition. Plasma Ca and magnesium tended to be higher (P < 0.10) in primiparous cows. The dietary Ca level did not affect the plasma osteocalcin (OC) level measured as bone formation or the urinary deoxypyridinoline (DPD) excretion measured as bone resorption before parturition in both primiparous and multiparous cows. After parturition, the plasma OC level was lower than it was before parturition in multiparous cows fed a low Ca diet, and in primiparous cows. There were no significant differences in urinary DPD excretion between each group before and after parturition. Both the plasma OC level and urinary DPD excretion of primiparous cows were significantly higher than those of multiparous cows in both the low and high dietary Ca groups. 相似文献