室内型胶合板天然耐久性的初步研究

作为一项反映材料优劣的重要的评价指标,天然耐久性直接决定了材料的使用寿命.笔者通过对胶合板胶合强度性能连续14年的跟踪测定,讨论和分析了胶合板的天然耐久性.最后总结出了胶合板在使用过程中随时间迁移其胶合强度性能随外界变化的规律,以期为胶合板的生产制备、生产工艺的改进及其应用领域的拓宽等方面起到一定的指导作用.     

山西特早熟玉米丰产栽培技术

山西省种植特早熟玉米的时间较短，农民缺乏种植经验，现将各地丰产栽培技术总结如下，供大家参考。     

In vitro Plant Regeneration from the Mature Tissue of Navel Orange (Citrus sinensis L. Osbeck) by Direct Organogenesis

An efficient in vitro regeneration system by direct organogenesis from mature nodal and internodal stem segments of Newhall navel orange (Citrus sinensis L. Osbeck) was developed. Illuminating conditions together with plant growth regulators affected the adventitious bud regeneration frequency and efficiency. The initial 15 d darkness inoculation is beneficial for the adventitious bud regeneration. The highest regeneration frequency (85.2%) and bud formation efficiency (3.7 per responsive internodal stem segment) were obtained in the media supplemented with 1.0 mg L-1 BAP and 0.5 mg L-1 NAA. ABA at 0.2 mg L-1 positively affected the bud formation efficiency, which amounted to 8.5 buds per internodal segment in the presence of BAP at 1.0 mg L-1. The adventitious shoots successfully rooted and were transferred to the soil.     

小家鼠胴体重指标划分种群年龄的研究

对2005年1~12月在贵州省息烽县鼠情监测点捕获的258只小家鼠标本,以胴体重为指标,参照繁殖状况,用胴体重频次分配方法将小家鼠划分为4个年龄组,其划分标准为,幼年组(Ⅰ):胴体重≤6.49 g;亚成年组(Ⅱ):6.50~9.99 g;成年组(Ⅲ):10.00~13.49 g;老年组(Ⅳ):≥13.50 g。不同年龄组繁殖力差异显著,胴体重随种群年龄的增长而不断增加,各年龄组胴体重与体重、体长、尾长均呈正相关性,且与体重相关性达极显著。研究认为,采用胴体重划分小家鼠年龄是可行的,方法简便,易于操作。     

胚胎干细胞及种系嵌合体的研究进展

胚胎干细胞是着床前的囊胚内细胞团或早期胎儿的原始生殖细胞经体外分化抑制培养建立的多能性细胞系 ,具有与胚胎细胞相似的形态特征和分化潜能 ,体外培养时保持未分化状态 ,可以传代增殖。改变维持胚胎干细胞不分化的培养条件 ,胚胎干细胞可自发分化成多细胞结构。在一定诱导下 ,胚胎干细胞可向多个方向分化 ,并生成多种功能细胞。胚胎干细胞注入到胚泡期胚胎或与桑椹期胚胎聚合 ,可以参与包括性腺在内的各种组织的嵌合体的形成。胚胎干细胞在细胞分化与调控 ,胚胎发育 ,遗传病 ,肿瘤 ,免疫和组织或器官移植等研究中显示着广泛的应用前景。而种系嵌合体的获得是实现 ES细胞途径的决定步骤 ,低的种系嵌合率则是制约 ES细胞应用的关键。提高供体 PGCs在受体生殖腺中的比例 ,缩短 ES细胞的体外培养时间 ,以及注入早期发育阶段的受体胚胎等都能提高种系嵌合率。文章从多个方面综述了胚胎干细胞的最新研究成果 ,并着重以禽类 ES细胞为例论述了种系嵌合体的检测方法 ,种系嵌合率的影响因素以及提高种系嵌合率的方法     

Foot-and-mouth disease eradication efforts in the Republic of Korea

On March 20, 2000, a suspected vesicular disease in cattle was reported to the National Veterinary Research and Quarantine Service (NVRQS) of the Republic of Korea. This represented the index case of a foot-and-mouth disease (FMD) outbreak, which spread through several provinces. The Republic of Korea had been free of FMD for 66 years prior to the reintroduction of the virus and had recently suspended imports of pork and pork products from neighboring Japan owing to a reported FMD outbreak in that country. The Korean outbreak was ultimately controlled through the combination of preemptive slaughter, animal movement restrictions, and a strategy of ring vaccination. The purpose of this paper is to review the current FMD situation in Korea in the aftermath of its 2000 epizootic and how it may affect future efforts to eradicate or reduce risk of reintroduction of the disease into Korea.     

丝核菌细胞核染色技术的研究

比较了5种不同的染色方法对丝核菌（Rhizoctonia)菌株细胞核的染色效果。结果表明，番红O－KOH的染色效果最好，Giemsa、苯胺蓝－甘油次之，染色效果最差的是苯胺蓝－乳酚油及曲利苯蓝－乳酚油。用番红O－KOH染色方法对分离自13种作物的57个丝核菌菌株进行细胞核染色，其中3个菌株为双核丝核菌，其余54个菌株为多核丝核菌。     

Analysis on Mineral Element Contents in Associated with Varietal Type in Core Collection of Yunnan Rice

Eight-element contents of 653 unpolished rice samples harvested from Xingping experiment farm, Yunnan Province under the same ecological conditions were analyzed by ICP-AES method. The mineral elements content were closely related to low diversity, high-yielding, and multi-resistance breeding; The K, Mg, Ca and Mn content in high-yielding and resistant varieties were high, and other nutrients such as P, Fe, Zn and Cu were low, which was connected with the heredity and physiological mechanism of mineral nutrients. There is zonal distribution of mineral elements content from Yunnan rice, especially for P, Fe, Zn and Cu co-related with the diversity center, paddy versus upland, glutinous and non-glutinous, glume-hair versus nuda, rice color, rice flavor, soft rice versus non-soft rice, but it did not find any association with indica-japonica types. The results supported the ecological variety group view of 5-grade taxonomic system “species-subspecies-ecological groups-ecological variety groups - varietal types“ .     

提高湖南省杂交棉种质量水平的商榷

在市场经济迅速发展、种子生产、流通量不断增加和加入WTO的新形势下，充分提高湖南省棉种的质量水平，推动棉种产业化的健康发展，对农民增收、农村稳定具有重大的现实意义。本文简要介绍了提高湖南棉种质量水平的紧迫性和可行性。在此基础上提出了提高棉种质量水平促进产业化发展的几点建议。     

表达HAV结构基因的重组腺病毒鉴定及细胞病变分析

利用RT-PCR方法,从HAV-L8株的RNA中扩增出结构基因(vp3 vp1),克隆到穿梭质粒pXCX2NotI上。采用磷酸钙-DNA共沉淀技术,将腺病毒载体pJM17与pXCX2-CMV-HAV共转染293细胞。在光学显微镜下观察到明显的细胞病变(CPE);经RT-PCR、免疫荧光染色和蛋白印迹鉴定证明HAV的结构基因已插入到腺病毒基因组中;在电子显微镜下观察发现有二十面体的病毒颗粒。以上结果表明获得了重组腺病毒rAdHAV,纯化后的rAdHAV滴度为1×109.0TCID50/mL。