新城疫病毒F48E9感染CEF细胞特异性表达基因的筛选鉴定

分别以NDV F48E9和La Sota株感染鸡胚成纤维细胞,于感染后8 h提取NDV感染CEF细胞总RNA,通过mRNA差异显示技术筛选病毒感染诱导表达的特异性基因.主要方法是先以锚定引物经反转录后,然后以9～10 bp随机引物为上游引物,以锚定引物Oligod(T)18为下游引物,进行PCR扩增,PCR产物采用8%的尿素变性PAGE胶电泳进行分离鉴定.对于特征性差异带进行第二次PCR扩增,克隆后测定其核苷酸序列.结果我们发现数个差异条带,选取差异带在500 bp以内的条带,经测序后显示经NDV F48E9感染后,有一条差异条带特异性表达的基因所编码的蛋白与Receptor(TNFRSF)-interacting serine-threonine kinase 2有47 %同源,是一个新基因,其蛋白功能不明确,有可能和细胞的凋亡有关.     

Overexpression of neuronal ARNT2 is involved in neuronal apoptosis evoked by low K+

AIM: To observe the expression of neuronal Aryl hydrocarbon receptor nuclear translocator 2 (ARNT2) involved in neuronal apoptosis evoked by low K+ and to investigate the relationship between ARNT2 and neuronal apoptosis. METHODS: After neuron apoptosis model was established, the changes of mRNA and protein of ARNT2 during apoptosis were investigated by RT-PCR and Western blotting, respectively. Immunofluorescence was analyzed by confocal microscopy to probe the subcellular localization of ARNT2. RESULTS: Induced by low K+, the expression of ARNT2 mRNA was up-regulated obviously at the point of 30 min, and peaks at the point of 1 h. This up-regulated expression lasted for 12 h, and the variation of ARNT2 protein was similar to that of mRNA. The results of immunofluorescence analyzed by confocal microscopy showed that the localization of ARNT2 protein was in the nucleus. CONCLUSION: ARNT2 locate in nuclei of normal cerebellar granule neurons of rat. During the process of apoptosis evoked by low K+, both mRNA and protein of ARNT2 are overexpressed.     

秦岭南北地区人口增长对水资源影响的比较研究

采用近30年来(1970-2000)关中地区33个县市、陕南地区26个县市气象观测数据及研究区内各主要县市的近50年来(1949-2000)人口数据,对比分析了人口增长对秦岭南北地区水资源的影响。分析表明近50年来,关中地区每年平均增长人口27.1万人,陕南地区每年增长人口8.9万人,关中地区人口总数和增长速率分别是陕南地区的2倍和3.5倍;关中地区和陕南地区的人均水资源量变化都呈现出下降的趋势,关中地区的下降速率为每年2.1%,陕南地区下降的速率为1.4%;从时间上看,关中地区90年代与60年代相比,人均水资源量减少了46.5%,陕南地区90年代与60年代相比,人均水资源量减少了16.5%;从空间上看,关中地区主要缺水区域位于渭河的中下游,大部分地区人均水资源量在1,400m3以下,而陕南地区只有汉中水资源受人类影响较大人均水资源量在1,400m3以下,其他县市水资源仍然很丰富,人均水资源量在2,800m3以上。由于人口增长迅速,关中地区的水资源较陕南地区在时间和空间上都存在严重的缺乏,前景不容乐观。因此,从人口和水资源两方面考虑陕南地区是支持关中经济发展的重要后方。     

Inhibitory and promotive allelopathy in rice (Oryza sativa L.)

Studies on allelopathic rice, which inhibits the germination and development of weeds such as barnyardgrass ( Echinochloa spp.) and ducksalad [ Heteranthera limosa (Sw.) Wild.], have been strongly conducted in the USA, the Philippines, Japan, Korea, and other countries since the 1980s. Weed-suppressing rice plants can compete with weeds for light and mineral nutrients by their rapid and thick growth characteristics, and probably also by their allelopathic effects. It was suggested that allelopathic properties were inherited from parents. Different screening methods for finding allelopathic rice simply and rapidly have been proposed. In contrast, stimulative allelopathy in rice, which promotes germination of the dormant and dormancy-awakened seeds of Monochoria vaginalis (Burm. f.) Kunth var. vaginalis , has been investigated by the authors since the 1990s. Rice seeds promoted the germination in the dark and in light under submerged conditions. Our experiments suggested that rice seeds, hulls, plants and straw contain stimulative substance(s) that promote seed germination of M. vaginalis .     

塔里木河干流上游土地利用动态变化研究

在干旱区 ,水资源十分匮乏。若能有效的控制塔里木河上游耕地面积的扩张 ,同时提高水资源利用率 ,则能解决塔里木河流域长期以来农业用水和生态用水之间的矛盾 ,这对塔里木河流域持续发展有重要意义。应用遥感技术进行上游土地利用动态变化则为上游耕地现状和趋势发展提供一种快速和准确的方法。本文利用 1 95 9年、1 983年、1 992年和 1 996年四个时期的航片及卫片资料 ,研究了塔里木河干流上游典型图幅土地利用的动态变化。结果表明 ,研究区域人工绿洲和居民点面积逐年扩大 ,湖泊面积明显缩小 ,而天然植被面积趋于缩小 ,难利用面积变化不大。为此 ,有关部门应严格控制目前这种耕地继续扩张趋势 ,同时加强植被生态环境建设     

稻田蜘蛛混合种群空间分布的动态研究

稻田蜘蛛混合种群的空间分布在水稻的不同发育期存在差异。其空间分布主要由稻田蜘蛛本身的扩散程度及生殖 ,游猎型和定居型数量的多寡 ,主要目标害虫的数量及其分布 ,栖息环境的变化及其耕作方式和稻田蜘蛛的种群密度及其种间竞争程度决定。     

樟子松松针锈病病原菌——春孢子形态及酯酶同工酶变异

樟子松松针锈病病原菌——鞘锈菌（Ｃｏｌｅｏｓｐｏｒｉｕｍ）的分类历来很乱。传统的分类方法都是依冬孢子形态和冬孢子寄主来进行的。但近年的研究表明１种鞘锈菌的春孢子可以侵染２种或２种以上的冬孢子寄主，所以按冬孢子寄主来划分鞘锈菌的种是不合理的。文中在病害症状观察和春孢子表面形态观察的基础上，首次对樟子松３种不同形态的春孢子的酯酶同工酶进行了分析，从而为鞘锈菌的分子分类学研究奠定了基础。     

磷肥用量对甜玉米磷素吸收利用的影响

Understanding the phosphorus (P) absorption mechanism is the premise for high P utilization and high crop yield. In this study, the effects of different P rates (0, 37.5, 75, 150, and 300 kg hm^-2, expressed as P0, P1, P2, P3, and P4, respectively) on sweet corn yield, biomass and P accumulation and tissue distribution, P transportation and P utilization were studied using a two-year P gradient positioning field experiment. Our results showed that P application significantly increased the fresh ear yield of sweet corn, but the yield difference among different P application rates (P1-P4) was not significant from 2018 to 2019 compared with P0 treatment. P application significantly increased the biomass and P accumulation of sweet corn plants at jointing, silking and fresh eating stages, respectively. In addition, P accumulation in grain was not significant different, which accounting for 42% of plant P accumulation. With P application, the contribution rate of P assimilation to the ear P accumulation was 57.3%-93.0% after anthesis, while the fresh ear yield per P accumulation, P physiological efficiency, agronomic efficiency, recovery efficiency and partial productivity decreased with P rates. Considering the yield and utilization efficiency of phosphate fertilizer, the supply of 37.5 kg hm^-2 of phosphate fertilizer can meet the needs of high yield and efficient utilization of phosphate fertilizer in sweet corn in this experiment.     

电力企业改革与政府管制

从电力企业自然垄断的客观规律性及所产生的问题分析入手,对我国电力企业的产业化分割和政府管制进行研究.研究认为 ,建立有序的市场和充满活力的经济,就必须对"电力生产企业"实施电价规制,处理好电力企业改革与政府管制的关系问题.     

MC4R基因主效位点在中外猪种中的遗传多样性及其与生长肥育性状的关联性分析

采用PCR-TaqI-RFLP技术,检测了9个中国地方猪种、3个外来猪种和1个培育猪种的612个个体及白色杜洛克×二花脸资源家系418头F2个体在MC4R基因D298N主效位点的遗传变异,统计分析了MC4R基因型与日增重、腹脂率及肩、胸、腰、臀部膘厚等生长肥育性状的关联性。结果表明:(1)G等位基因在中国地方猪种、长白和杜洛克中呈高频分布,而大白和南昌白猪具有高频率的A等位基因;(2)GG基因型个体的胸、腰、臀部膘厚较AA基因型和AG基因型个体厚,GG基因型个体的240日龄重、至240日龄平均日增重大于AA基因型个体,GG基因型个体的腹脂率高于AA基因型个体,差异显著。试验结果进一步验证了MC4R基因对猪生长肥育性状的影响效应。