芦荟饮料褐变的控制研究

研究了芦荟凝胶在制汁过程及芦荟饮料在加工过程中温度和时间等因数对芦荟饮料色泽的影响。结果表明,搅汁时间10min,杀菌温度85℃,杀菌时间25min是有效控制芦荟饮料褐变的最佳工艺参数。     

猪结合珠蛋白基因的克隆、原核表达及单克隆抗体制备

本研究的目的在于利用重组猪结合珠蛋白(Haptoglobin,Hp)制备特异性单克隆抗体.以梅山猪肝脏总RNA为模板,利用RT-PCR方法扩增猪hp基因,将其克隆到原核表达栽体pGEX-KG和pET-32a上,转化至E.coli BL21中,IPTG诱导下高效表达重组GST-Hp和HIS-Hp蛋白.用纯化的重组GST-Hp免疫BALB/c小鼠,用纯化的HIS-Hp作为包被抗原进行间接ELISA检测,通过淋巴细胞杂交瘤技术筛选阳性细胞株,制备单克隆抗体.结果表明筛选到2株高效分泌抗猪Hp单克隆抗体的杂交瘤细胞株,命名为3B4和3C8.Western-blot和间接ELISA检测均表明制备的单克隆抗体具有良好的特异性.     

Effect of Steeping Conditions on Wet-Milling Characteristics of Hard Red Winter Wheat

A batch-wise small-scale wet-processing laboratory for whole wheat kernel has been designed and constructed to produce wheat starch and gluten from wheat grains. Hard red winter wheat kernels were steeped in three steeping media: SO₂ solution, lactic acid, and hydrochloric acid. Acid concentrations of 0.1, 0.3, and 0.5%, were used for SO₂ solutions and hydrochloric acid, and 0.1, 0.6, and 3.0% for lactic acid. After 16, 20, and 24 hr of steeping, the wheat was wet-milled. Yields and protein contents of wet-milling fractions were compared. Both high concentration of steeping media and long steeping time increased the starch yield and decreased the protein contents of the starch. However, the steeping time and acid concentration could be reduced from 24 to 20 hr and from 0.5 to 0.3%, respectively, without any statistically significant difference in starch yields or protein contents of the starch. Consistency and color of the starch were affected by both steeping time and acid concentrations of steeping media.     

田面微地形对滴灌灌水均匀性的影响

首先分析了田面微地形对滴头实际工作压力的影响,田面越不平整,滴头实际工作压力与设计工作压力的差值越大,当田面局部高差大于滴头设计工作压力时,滴头流量等于零;然后提出了田面微地形产生的流量偏差率公式,并用泰勒级数展开;最后利用描述统计方法和独立样本T-检验的方法对余项进行分析,置信度为99%的余项取值区间为0.001￣0.002,说明余项能够被忽略,田面微地形产生的流量偏差率公式可以用简单的形式来表示。     

Factors affecting the adoption of agroforestry practices by farmers in Cameroon

This paper presents empirical evidence on the impact of socio-economic factors on the adoption of agroforestry practices in Cameroon. The analysis uses primary farm-level data collected from June to December 1996. Three major provinces of the country were covered, namely Centre, Southwest and Northwest Several agroforestry technologies have been promoted among farmers in the zone, including alley farming, improved fallow, live fencing, cut-and-carry fodder and apiculture. The status of adoption of each agroforestry practice is described and factors that affect adoption identified. These are gender of farmer, household family size, level of education, farmer’s experience, membership within farmers’ associations, contact with research and extension, security of land tenure, agroecological zone, distance of the village from nearest town, village accessibility and income from livestock. Research findings indicate that since factors affecting farmers’ adoption of agroforestry practices differ across techniques, generalisation is to be avoided.     

综合加固法处理沿海滩涂软基施工

采用水泥粉喷柱、砂砾垫层与覆膜土工布三者结合的综合加固法处理滨海相淤泥沉积,水泥粉喷桩的施工及成桩检测;土工布的施工;软基处理应注意的问题。     

大花蕙兰幼苗叶片诱导类原球茎

以大花蕙兰无菌幼苗叶片为外植体,成功诱导类原球茎(PLB).叶片部位是影响诱导的关键,只有叶片基部片段能成功诱导出PLB.不同激素配比以及外植体来源对PLB诱导有重要的影响.低浓度的2,4-D和较高浓度的6-BA对于拟球茎诱导PLB具有显著的促进作用.当2,4-D浓度高于0.6 mg/L时,PLB诱导受到显著抑制.较高的2,4-D水平可能有利于PLB诱导初始阶段,但不利于PLB诱导中后期的进一步发育.不同基因型有不同的最佳激素组合.添加0.2 mg/L的NAA对大花蕙兰PLB诱导没有显著影响,但在有的基因型中对PLB诱导有促进作用.以叶片基部片段诱导大花蕙兰PLB是可行的,为转基因研究提供了再生技术基础.