基施硒钴配合肥对苜蓿生产性能的影响

经过2006-2008年为期3年在硒钴缺乏的河南省郑州市黄河滩区苜蓿(Medicago sativa L.)草地上基施硒钴配合肥料,研究对其生产性能的影响,以期改善苜蓿生长环境和提高苜蓿产量和品质。结果表明,硒钴配合基施均能够显著提高现蕾到开花期苜蓿叶面积指数和净光合能力(P<0.05),提高苜蓿单位叶面积生产干物质的速率,有利于苜蓿的花前青干草生产;在4种硒钴配施肥中,以硒量570 g/hm²、钴量762 g/hm²配施效果最显著,2年内牧草平均增产8.0%(P<0.05),种子的千粒重提高了12.5%(P<0.01),耕作层地下生物量提高了4.6%。     

不同的干燥工艺对荷花蜂花粉功能成分的影响

探讨了5种干燥工艺对荷花蜂花粉功能成分的影响。微波干燥的干燥效率最高,60%火力处理540s可使水分从31.41%降到6.42%;干燥方式对荷花蜂花粉蛋白质影响较小;微波干燥后总黄酮提取率最高,为13.20mg/g;真空冷冻干燥后维生素C和过氧化氢酶含量最高,分别为48.89mg/100g和29.86mg/g·min。总的来说,真空冷冻干燥对荷花蜂花粉的功能成分影响最小;常压恒温干燥对功能成分影响较小、工艺简单、对设备要求低,宜于大规模应用于实际生产中。     

硒肥对莜麦生产性能的影响

在河南省黄河滩区种植莜麦(Avena nuda)的地段上播前基施硒肥,研究硒肥(Na2SeO3·5H2O)用量在570～1143 g· hm-2时,对莜麦生产特点和生产性能的影响,探索提高莜麦生产水平的新途径,进一步丰富河南优质饲草资源.结果表明:对莜麦生产适宜的硒肥基施量为765～954 g·hm-2,基施硒肥能使莜麦的鲜干比平均降低6.69％ (P＜0.05),茎叶比平均提高10.01％ (P＜0.05),分蘖数平均提高12.50％ (P＜0.05),扬花期干草产量平均提高8.22％ (P＜0.05);另外,硒肥还能显著提高种子的千粒重,但不能提高莜麦果实产量.因此,合理基施硒肥是提高莜麦     

应用致敏SPA菌体提高CAV/CPV PCR检出率的研究

为消除粪便等病料中的PCR干扰物质 ,提高犬腺病毒与细小病毒 (CAV CPV)联合PCR检出率。根据免疫吸附的原理 ,应用CAV CPV高免血清致敏含A蛋白的金黄色葡萄球菌 ,制成致敏SPA菌体免疫吸附剂 ;以此免疫吸附提取粪便等病料中的CAV/CPV ,然后直接或经 3 5mol/LMgCl2 将所吸附病毒洗脱后再做PCR。结果 8份经电镜负染或HA/HI试验验证为阳性的粪便样品 ,如此处理后进行PCR检测 ,结果均为阳性 ;而直接取粪便离心后用上清进行PCR检测 ,结果均为阴性。表明该法可有效去除待检样品中的PCR干扰物质 ,提高PCR的检出率。     

2010年中国小麦市场回顾与2011年行情展望

全球谷物市场在2011年春节期间的整体走势可谓异常火爆．从节后郑州强麦期货市场首日涨停的“飞天舞”．到国际市场上饲用和优质小麦、期货和现货小麦价格的“比翼双飞”组合．使得国内农户更倾向于挺价待涨。其主要利多题材来自于2010年全球范围内多发的灾害性天气．     

水貂阿留申病毒的分子生物学研究进展

从水貂阿留申病毒(ADV)基因组特点出发,就阿留申病毒的分子生物学研究进展作以简单综述。     

混合碱茅在河南省中度盐渍化土壤中改良效果的研究

对混合碱茅在河南省黄淮海平原盐渍化潮土中进行了连续4年的应用性试验和研究.结果表明,混合碱茅显著地改善盐渍化土壤的结构,降低土壤表层中盐份含量,提高土壤的保肥和供肥能力,改善理化性质,促进后茬牧草的高产.     

我国牛轮状病毒的分离鉴定和疫苗研究进展

约50%的犊牛腹泻是由牛轮状病毒(Bovine Rotavirus, BRV)引起的,它制约着我国养牛业的发展.论文综述了牛轮状病毒毒株的分离、鉴定方法及疫苗研制的现状,并对BRV新型疫苗的发展趋势进行了展望.     

一种副猪嗜血杆菌新型分型方法——多基因位点序列分型

副猪嗜血杆菌(Haemophilus parasuis,Hps)为革兰氏阴性菌,属于巴氏杆菌科嗜血杆菌属,是猪副猪嗜血杆菌病的主要致病菌.应用多基因位点序列分型技术对副猪嗜血杆菌进行分型,具有简便快速、重复性强、分辨率较高、所得数据标准等优点.并且还可通过互联网实现实验室间数据共享及比较.通过试验对多基因位点序列分型(MLST)技术的原理、方法及结果进行了阐述.以期为副猪嗜血杆菌的分型提供参考.     

Serum biochemical parameters and amino acids metabolism are altered in piglets by early-weaning and proline and putrescine supplementations

The study was to investigate the effect of early-weaning stress and proline (Pro) and putrescine (Put) supplementations on serum biochemical parameters and amino acids (AA) metabolism in suckling and post-weaning pigs. Blood and small intestinal mucosa were harvested from suckling piglets at 1, 7, 14, and 21 d of age and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age, as well as from piglets received oral administration of Pro and Put from 1 to 14 d old. In suckling piglets, the serum glucose, albumin and total cholesterol levels were increased (P < 0.05) with increasing age, whereas the serum globulin, urea nitrogen (BUN), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) levels were lowered (P < 0.05). The concentrations of most serum AA and the AA transporters related gene expressions were highest in 7-d-old piglets (P < 0.05), whereas the phosphorylation status of the mammalian target of the rapamycin (mTOR) signaling pathway in the small intestine increased in piglets from 1 to 21 d old (P < 0.05). Weaning at 14 d old increased (P < 0.05) the BUN and triglycerides levels in serum, as well as jejunal solute carrier family 7 member 6 (SLC7A6), ileal SLC36A1 and SLC1A1 mRNA abundances at d 1 or 3 post-weaning. Weaning also inhibited (P < 0.05) the phosphorylation levels of mTOR and its downstream ribosomal protein S6 kinase 1 (S6K1) and 4E-binding protein-1 (4EBP1) in the small intestine of weanling pigs. Oral administration of Put and Pro decreased (P < 0.05) serum ALP levels and increased (P < 0.05) intestinal SLC36A1 and SLC1A1 mRNA abundances and mTOR pathway phosphorylation levels in post-weaning pigs. Pro but not Put treatment enhanced (P < 0.05) serum Pro, arginine (Arg) and glutamine (Gln) concentrations of weaning-pigs. These findings indicated that early-weaning dramatically altered the biochemical blood metabolites, AA profile and intestinal mTOR pathway activity, and Pro and Put supplementations improved the AA metabolism and transportation as well as activated the intestinal mTOR pathway in weanling-pigs. Our study has an important implication for the broad application of Pro and Put in the weaning transition of piglets.