银杏北方栽培技术初探

探索高纬度地区栽培银杏树技术,考察其成活率,及生长条件。采用2年龄苗木移栽。当年秋季带土装盆放入室内,存放温度5-10℃,湿度50％-75％。次年春,移回大地。以后冬季不用移回室内,成活率94％以上。根据银杏树的生长规律,采取科学的管理方法,在北纬43°51’,东经125°18’的北温带地区银杏树生长良好,对高纬度地区栽培银杏树具有一定指导作用。     

开食料补饲日龄对羔羊瘤胃和小肠组织形态的影响

为研究开食料补饲日龄对羔羊瘤胃和小肠组织形态的影响,选用72只体重接近的湖羊初生公羔(双羔,初生重3.51±0.52 kg),随机分为2组,7或42 d补饲开食料,每组36只羔羊。分别在14,28,42,56,70和84 d每组各屠宰6只羔羊,采集瘤胃和小肠组织样品。结果表明:42 d时7 d补饲组瘤胃背囊和腹囊乳头高显著高于42 d补饲组(P<0.05),42 d时7 d补饲组瘤胃腹囊乳头宽有大于42 d补饲组的趋势(P=0.053),42 d时7 d补饲组背囊肌层厚显著高于于42 d补饲组(P<0.05);28和42 d时7 d补饲组十二指肠和回肠绒毛长均显著高于42 d补饲组(P<0.05),42 d时7 d补饲组回肠隐窝深显著低于42 d补饲组(P<0.05),42 d时7 d补饲组十二指肠和回肠绒毛长和隐窝深比显著高于42 d补饲组(P<0.05)。结论 7 d补饲有利于瘤胃组织形态发育和42 d前小肠粘膜发育。     

缓控释肥不同施用量和施用方式对旱作区全膜马铃薯生长及产量的影响

为掌握缓控释肥的施用对全膜马铃薯关键生长指标及产量的影响,分别在高(900 kg·hm-2)、中(750 kg·hm-2)、低(600 kg·hm-2)3种施肥条件下进行了2种不同施用方式即集中沟施(F)与表层撒施(B)的田间试验,并测定了马铃薯主要生长指标和产量。结果表明:缓控释肥施用量对旱作全膜马铃薯主要生长指标、产量及商品率的影响均达显著性差异水平,在施用900 kg·hm-2的高肥量时,其沟施处理HF的马铃薯产量、大薯率和商品率均达到最大,分别达43 608.85 kg·hm-2、72.1%和92.7%。施用方式对马铃薯地下主要生长指标、产量和大薯率均表现出显著性差异,且在3种施肥量下,F处理的马铃薯产量、大薯率和商品率均高于B处理,其中在高肥水平下F处理较B处理平均增产达2 834.54 kg·hm-2,增产率为6.4%。施用量×施用方式对马铃薯各生长指标和产量影响均不显著。综合来看,缓控释肥在较高水平的施肥(900 kg·hm-2)条件下,集中沟施处理有利于旱作区全膜马铃薯产量的提高。     

栖架舍饲散养模式对蛋鸡生产性能、蛋品质及免疫机能的影响

本研究根据蛋鸡的生物学特点和行为学习性,在舍内配置栖木、采食底网、料线、水线及产蛋箱等规模饲养设施,构建了栖架舍饲散养模式,围绕其对蛋鸡生产性能、蛋品质与免疫机能的影响,进行了初步的应用研究。结果表明,栖架散养组开产时间推迟3周,但其平均料蛋比下降了9.05%,平均蛋重、平均产蛋率较对照组分别提高了2.62%、3.45%;栖架舍饲散养组的蛋白高度、哈氏单位、蛋形指数均显著高于对照组(P<0.05),分别提高了24.13%、8.03%、6.35%;栖架舍饲散养组的胸腺指数、脾脏指数、法氏囊指数、鸡新城疫抗体效价较对照组分别提高了5.44%、3.48%、6.57%、16.74%。综上所述,栖架舍饲散养模式有助于降低蛋鸡料蛋比,改善蛋品质,促进免疫机能的提高,体现了福利化健康养殖的需要。     

影响草坪生长的因子分析

针对杨凌地区3个草坪品种的8项指标进行了调查研究,对得出的27组数据进行了主成分分析,得出影响草坪生长的前3个主成分分别为人为管理因子、光照因子、水分因子.这3个因子对草坪生长的累计贡献率达87.449%.其中第一主成份(人为管理因子)中.后期管理、品种、密度等3个因子对其影响最大;第二主成份(光照因子)中,颜色、盖度、品种等3个因子对其影响最大;第三主成份(水分因子)中,土壤含水量、品种、盖度等3个因子对其影响最大.     

柳枝稷TIFY基因家族的鉴定与分析

为探究TIFY基因家族在柳枝稷（Panicum virgatum）中的生物学功能,本研究运用生物信息学手段对柳枝稷基因组中的TIFY家族成员进行了鉴定,并对其染色体定位、进化与结构特点、启动子区顺式作用元件、时空表达模式等进行了分析,且通过定量实验验证了JAZ亚家族部分成员的激素应答与胁迫响应特性。结果表明：48个柳枝稷TIFY基因分属于ZML（7个）、TIFY（1个）与JAZ（40个）3个亚家族,分布在除染色体3以外的染色体上,且在染色体9成簇排列;柳枝稷TIFY基因按照进化关系及结构特点可分为5组,每组内的成员具有相似的基因结构与保守基序组成;柳枝稷TIFY基因启动子区存在多种胁迫响应元件与激素信号调控元件,经定量实验证实PviTIFY10各成员在茉莉酸（jasmonic acid,JA）处理及盐与干旱胁迫下反应强烈;JAZ亚家族部分成员尤其是PviTIFY10b在花序与根的发育过程中有较高表达。根据以上结果推测TIFY基因家族可能在柳枝稷激素信号调控与胁迫防御反应及生长发育等多方面发挥功能。     

一种新的豇豆根腐病病原菌鉴定及室内药剂筛选

本文对四川成都发生的豇豆根腐病病原菌进行鉴定,为豇豆根腐病的药剂防治提供依据。采集了成都市豇豆种植区豇豆根腐病病株,经常规组织分离并纯化获得116株分离菌株,对分离菌株进行致病性测定、形态学鉴定及rDNA-ITS序列分析。结果表明,分离到的镰孢菌中有10株为Fusarium commune,分离频率为8.62%。对该10株菌进行致病性测定,均为致病菌株;其中MW4-11菌株致病性最强。该致病菌株的最适生长温度为25~30℃,菌丝致死温度为70℃。选用7种杀菌剂通过平皿培养法对MW4-11菌株进行室内毒力测定。结果表明,75%肟菌·戊唑醇水分散粒剂对该致病菌株的毒性最强,EC50为0.030μg/mL。     

MADS-box转录因子在果实成熟及品质形成中的调控作用研究进展

近年来关于MADS-box转录因子的研究已陆续从花器官的形成扩展至植物生长发育的全过程,其中参与果实成熟与品质调控是研究的热点之一。综述了MADS-box转录因子参与调控果实品质形成的研究进展,讨论了该基因家族在果实品质调控研究方面存在的主要问题,明确了其未来研究的主要方向。     

Computational methods in super-resolution microscopy

The broad applicability of super-resolution microscopy has been widely demonstrated in various areas and disciplines. The optimization and improvement of algorithms used in super-resolution microscopy are of great importance for achieving optimal quality of super-resolution imaging. In this review, we comprehensively discuss the computational methods in different types of super-resolution microscopy, including deconvolution microscopy, polarization-based super-resolution microscopy, structured illumination microscopy, image scanning microscopy, super-resolution optical fluctuation imaging microscopy, single-molecule localization microscopy, Bayesian super-resolution microscopy, stimulated emission depletion microscopy, and translation microscopy. The development of novel computational methods would greatly benefit super-resolution microscopy and lead to better resolution, improved accuracy, and faster image processing.     

Effects of Concentrate Protein Levels on Growth and Development of Xinong Saanen Dairy Goat Lambs

The objective of this study was to investigate the effects of different crude protein (CP) levels of concentrate on the growth and development of Xinong Sannen dairy goat lambs.60 lambs(40 male and 20 female) with good similarity in age (5 days) and birth weight were selected and randomly allocated to 4 groups. The groups M16 and M20 were males with 20 lambs per group,groups F16 and F20 were females with 10 lambs per group. The groups M16 and F16 were fed supplementary concentrate with 16% CP,the others were fed supplementary concentrate with 20% CP. The test lasted for 18 weeks,and the weight and body size indexes of the lambs were measured every 2 weeks,and the blood samples were collected and the biochemical indexes were analyzed. The results showed that the weight of lambs in 20% CP group had an increaseing trend than that in 16% CP group,and that was more apparent in male lamb. The body height,rump height,rump length,body length,chest depth and chest circumference of lambs in 20% CP group were greater than that of the 16% CP group.Male lambs seemed to have a faster tendency than female lambs in growth and development during the trial period.There was no significant effect on most of blood biochemical indexes among different CP levels groups (P >0.05).High CP level diet could easily trigger diarrhea.So,the feeding amount with high CP level concentrate should be controlled during lamb cultivation.Compared with 16% CP in concentrate,the 20% CP was more beneficial to grow and develop to the lambs.